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MetabolomicsAspSnFR: A Genetically-Encoded Biosensor for Real-Time Monitoring of Aspartate in Live Cells

Aspartate is crucial for nucleotide synthesis, ammonia detoxification and maintaining redox balance via the malate-aspartate-shuttle (MAS). To disentangle these multiple roles of aspartate metabolism, tools are required that measure aspartate concentrations in real time and in live cells. We intr...

2024-08-27 | MTBLS10035 | MetaboLights
TranscriptomicsReal-time Monitoring of Cisplatin Toxicity on Cancer Cells
Treatment of MCF7 breast cancer cells by cisplatin leads to a very specific metabolic response and an onset of cell death about 10-11 h after beginning of treatment. For more detailed understanding of the molecular processes underlying the specific metabolic response, mRNA was isolated from MCF7 cel...
ORGANISM(S): Homo sapiens 
2011-04-04 | E-GEOD-28274 | biostudies-arrayexpress
ProteomicsHypocrates, a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. ...
ORGANISM(S): Escherichia coli 
2021-12-15 | PXD029624 | Pride
Fluorescence Biosensor Visualization Hypohalous acids Ratiometric 
GenomicsBacterial biosensor screening
Bacterial biosensor screening
PRJEB66358 | ENA
ProteomicsMeasuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor
Metabolic heterogeneity modulates productivity, antibiotic resistance and cancer aggressiveness. Since metabolic fluxes represent the functional output of metabolism, with glycolytic flux correlating with highly-productive phenotypes and cancer, such flux map will be indicative of the cellular metab...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-12-17 | PXD012964 | Pride
Yeast Mcherry Cggr 
ProteomicsTracking the dynamics of the glycolytic metabolite fructose 1,6-bisphosphate in liver cancer cells with a fluorescent biosensor
HYlight is a genetically encoded fluorescent biosensor that ratiometrically monitors fructose 1,6-bisphosphate (FBP), a key glycolytic metabolite. Given the role of glucose in liver cancer metabolism, we expressed HYlight in human liver cancer cells and primary mouse hepatocytes. Through in vitro, i...
ORGANISM(S): Homo sapiens (Human) 
2024-11-14 | PXD051879 | Pride
6-bisphosphate  hepatocellular carcinoma  metabolomics  biosensor Glycolysis  fructose 1 
UnknownDisposable Polyaniline/m-Phenylenediamine-Based Electrochemical Lactate Biosensor for Early Sepsis Diagnosis.
Not available
S-EPMC10892424 | biostudies-literature
TranscriptomicsPS19 brain lysate transfection changing ER stress gene expression in Biosensor cell
To investigate whether PS19 brain lysate transfection changes ER stress gene expression in Biosensor cell
ORGANISM(S): Homo sapiens 
2022-12-22 | GSE217525 | GEO
ProteomicsA BRET Biosensor for Measuring Uncompetitive Engagement of PRMT5 Complexes in Cells
Protein arginine methyl transferase 5 (PRMT5) plays a global role in cell physiology and is an established therapeutic target in cancer. In approximately 10-15% of human cancers, deletion of the methylthioadenosine phosphorylase (MTAP) gene results in accumulation of methylthioadenosine (MTA), expos...
ORGANISM(S): Homo sapiens (Human) 
2025-12-08 | PXD028138 | Pride
Nanobret - chemical proteomics – cell-based assay – sam – target engagement – thermal profiling 
GenomicsSaccharomyces cerevisiae strain:OrthoRep GPCR biosensor
Heterologous engineering of receptors using OrthoRep (HERO) for directed evolution of GPCRs in yeast
PRJNA1533496 | ENA
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