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ProteomicsmCherry contains a fluorescent protein isoform that interferes with its reporter function
Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that bi...
ORGANISM(S): Escherichia coli 
2022-04-19 | PXD032954 | Pride
Lc-msms Mcherry 
ProteomicsCryptococccus Rav1-mCherry LC-MS/MS
we performed in vivo pull-down experiment using the rav1Δ::RAV1-mCherry strain and RFP-trap agarose beads to precipitate Rav1-mCherry protein and its interacting partners.
ORGANISM(S): Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) 
2023-10-02 | PXD042074 | Pride
Rfp trap agarose beads Cryptococcus Rav1-mcherry Lc-ms/ms 
TranscriptomicsDynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line
Hearing loss is most commonly caused by the destruction of mechanosensory hair cells in the ear. This condition is usually permanent: Despite the presence of putative hair-cell progenitors in the cochlea, hair cells are not naturally replenished in adult mammals. Unlike those of the mammalian ear, t...
ORGANISM(S): Danio rerio 
2014-04-01 | E-GEOD-55853 | biostudies-arrayexpress
GenomicsMycoplasma GM12 mCherry expressing mutant strain
Mycoplasma GM12 mCherry expressing mutant strain
PRJEB99006 | ENA
TranscriptomicsThe organoid generated from murine small intestine: mCherry-positive vs. mCherry-negative
The organoids heterogenously expressed mCherry by the lentivirus transduction were dissociated into single cells. A single mCherry- positive and -negative cell were collected and grown up to the organoids, respectivery. The difference of the gene expression profile between mCherry- positive and -neg...
ORGANISM(S): Mus musculus 
2014-07-16 | GSE59425 | GEO
ProteomicsCryptococcus Wcp1-mCherry Wcp1-FLAG AP–MS
This study aims to identify Wcp1-interacting proteins in Cryptococcus neoformans under different pH conditions. Wcp1 is a WD40–cyclophilin fusion protein implicated in adaptation to host-associated stresses, including CO2, temperature, and acidic pH. Affinity purification coupled with liquid chrom...
ORGANISM(S): Fungi Cryptococcus neoformans H99-YPD_1 
2026-05-16 | PXD077146 | Pride
Wcp1 Cryptococcus Ph 
ProteomicsDifferential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants....
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-18 | PXD031787 | Pride
Arabidopsis Lc-msms Autophagosomes Affinity purification 
TranscriptomicsGene expression profilling of NEUROG2-mCherry lineage in cerebral organoids
To identify and purify NEUROG2-expressing cells and trace their short-term lineage, we engineered two NEUROG2-mCherry knock-in human embryonic stem cell (hESC) lines. Transcriptomic profiling of NEUROG2:mCherry knock-in hESC-derived cerebral organoids revealed an enrichment of neurogenic, oligodendr...
ORGANISM(S): Homo sapiens 
2024-12-19 | GSE277092 | GEO
GenomicsSindbis virus strain:TE12 | isolate:SINV-mCherry FMV2A
Sindbis virus TE12 mCherry FMV2A genome sequencing and assembly
PRJNA1266274 | ENA
GenomicsGene expression profilling of NEUROG2-mCherry lineage in cerebral organoids
Gene expression profilling of NEUROG2-mCherry lineage in cerebral organoids
PRJNA1160714 | ENA
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