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Metabolomicsliquid chromatography-mass spectrometry
Bacterial cells harvested at peak enzyme activity were subjected to untargeted metabolomic profiling to evaluate metabolic changes during straw degradation compared to control (CK) groups. Samples were extracted using pre-chilled methanol–water (4:1, v/v) and analyzed on a UHPLC-Q Exactive HF-X syst...
2026-08-24 | MTBLS15412 | MetaboLights
MetabolomicsMetabolomic Profiling of Mice Serum during Toxoplasmosis Progression Using Liquid Chromatography-Mass Spectrometry
Better understanding of the molecular changes associated with disease is essential for identifying new routes to improved therapeutics and diagnostic tests. The aim of this study was to investigate the dynamic changes in the metabolic profile of mouse sera during T. gondii infection. We carried out ...
2017-01-25 | MTBLS216 | MetaboLights
MetabolomicsHigh-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry (Mixed-mode column chromatography)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. RNA modifications play a key role in RNA folding and function and their ...

2023-10-26 | MTBLS2497 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one mL of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS844 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS849 | MetaboLights
MetabolomicsRapid chromatography combined with trapped ion mobility mass spectrometry for analysis of derivatized small molecules
Liquid chromatography (LC) separates biomolecules based on their physicochemical properties. Reproducible retention times support compound identification, while efficient separation of structurally similar compounds is essential for accurate quantification. However, conventional LC methods are often...
2026-06-30 | MTBLS14813 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method quantification)
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS848 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method development)
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS826 | MetaboLights
MetabolomicsCombining peak- and chromatogram-based retention time alignment algorithms for multiple chromatography-mass spectrometry datasets
Spring wheat (Triticum aestivum L.) was grown in a free-air carbon dioxide (CO2) enrichment (FACE) field experiment. Grain and biomass yield and its components were determined at maturity and the grain metabolome was analysed by gas chromatography-mass spectrometry (GC-MS) for elevated and ambient C...
2012-09-21 | MTBLS21 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Cell culture sampling)

One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using ...

2019-11-15 | MTBLS843 | MetaboLights
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