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MetabolomicsWhole genome CRISPRi screening identifies druggable vulnerabilities in drug-resistant Mycobacterium tuberculosis

Tuberculosis (TB) remains a global health threat, aggravated by the emergence of drug-resistant strains. This study aims to identify genes and metabolic pathways with increased vulnerability in drug-resistant Mycobacterium tuberculosis strains compared to their drug-susceptible counterparts. Usin...

2024-10-15 | MTBLS11277 | MetaboLights
MetabolomicsIn Vivo CRISPR Screens Identify SLC1A5 as a Metabolic Barrier for Cancer Immunotherapy
Tumor metabolic reprogramming profoundly shapes antitumor immunity, but the mechanisms linking glutamine metabolism to immune evasion in hepatocellular carcinoma (HCC) remain incompletely understood. Here, we identify the glutamine transporter SLC1A5 as a tumor-intrinsic regulator of HCC progression...
2026-08-20 | MTBLS15401 | MetaboLights
TranscriptomicsCRISPR screen exploring resistance to AKTi (Capivasertib) in ER+ Breast Cancer in MCF7, T47D and CAMA-1 cell lines
A genome-wide CRISPR-Cas9 knockout screen was performed in the breast cancer cell lines T47D, MCF7, and CAMA-1 to identify genes modulating sensitivity and resistance to capivasertib, a selective AKT inhibitor. Cells were transduced with a CRISPR library, followed by treatment with capivasertib or v...
ORGANISM(S): Homo sapiens 
2026-05-25 | E-MTAB-16438 | biostudies-arrayexpress
GenomicsAmplicon sequencing data of sgRNA cassettes from pig CRISPR screening time course to identify essential genes in PK15 and IPEC-J2 cell lines
This study describes a genome-wide CRISPR-Cas9 knockout (GeCKO) screen performed in two porcine cell lines, PK15 and IPEC-J2. A custom-designed porcine GeCKO (pGeCKO) library targeting protein-coding genes, lncRNAs, and miRNAs was used. Genomic DNA was harvested from GeCKO cell populations at four t...
ORGANISM(S): Salmo salar 
2025-08-30 | E-MTAB-15487 | biostudies-arrayexpress
TranscriptomicsscRNA-seq CRISPR screening of human posterior brain organoids
We used a pooled, multiplexed CRISPR/Cas9 gene knock-out (KO) experiment with single-cell transcriptomic readout to perturb and validate selected TF regulomes . We designed gRNAs and generated a pooled lentiviral library targeting 12 TFs with specific midbrain or hindbrain expression or high regulat...
ORGANISM(S): Homo sapiens 
2026-03-17 | E-MTAB-15826 | biostudies-arrayexpress
GenomicsThe ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation
CAMPARI2 CRISPR screening for SOCE modulators of ER stress. PC cells were sorted and sequenced for CRISPR whole KO library (De brie). Unsorted, SOrte din LOW PC and LOW PC Tunica treated fro 4hours were analysed.
ORGANISM(S): Mus musculus 
2023-12-09 | E-MTAB-13548 | biostudies-arrayexpress
GenomicsCRISPR-Cas9 screening of lncRNAs and protein coding genes involved in the transdifferentiation from human pre-B cells into macrophages
BLaER1 cells are human leukemia pre-B cells able to transdifferentiate into functional and non-tumorigenic macrophages. With the goal of uncovering the genes involved in the transdifferentiation process, we have designed a DECKO (Double Excision CRISPR Knockout) library to knockout lncRNAs and prote...
ORGANISM(S): Homo sapiens 
2022-04-25 | E-MTAB-10445 | biostudies-arrayexpress
Genomicswhole genome CRISPR Screening (Brunello library) on AMO-1 cell line treated with Ceritnib
AMO-1 cells were transduced with Brunello CRISPR screening library at MOI 0.3, and culture in the presnece of ceritinib. DNA was isolated and one step PCR was perfomed and gRNAs conferring to resistance or sensitivity was analysed using MAGECK-VISPR.
ORGANISM(S): Homo sapiens 
2025-12-15 | E-MTAB-15465 | biostudies-arrayexpress
TranscriptomicsCRISPR Screening in Glioblastoma
This is an in vitro genome-wide CRISPR/cas9 screen in human glioblastoma stem cells, screening for genes essential for survival of these cells. These cells express cas9 and have been transfected with a guide RNA library causing gene knockouts. We will analyse the sequencing data for depletion of gui...
ORGANISM(S): Homo sapiens 
2016-03-22 | E-ERAD-448 | biostudies-arrayexpress
GenomicsGlioblastoma CRISPR Screen
This is an in vitro genome-wide CRISPR/cas9 screen in human glioblastoma stem cells, screening for genes essential for survival of these cells. These cells express cas9 and have been transfected with a guide RNA library causing gene knockouts. We will analyse the sequencing data for depletion of gui...
EGAS00001001519 | EGA
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