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Seawater dissolved organic matter (DOM) is a large reservoir of carbon composed of a complex and poorly characterized mixture of molecules. Sponges have long been known to consume dissolved organic carbon (DOC) from this mixture, but the role of microbial sponge symbionts in this process is compl...

2022-03-11 | MTBLS2200 | MetaboLights

Seawater dissolved organic matter (DOM) is a large reservoir of carbon composed of a complex and poorly characterized mixture of molecules. Sponges have long been known to consume dissolved organic carbon (DOC) from this mixture, but the role of microbial sponge symbionts in this process is compl...

2022-03-11 | MTBLS2199 | MetaboLights
We used the resolving power of single-cell transcriptional profiling to molecularly characterize the mouse adipose stem and progenitor cell-enriched, subcutaneous adipose stromal vascular fraction. We molecularly assessed CD45- CD31- SVF cells using the 10x Genomics Chromium (10x) platform.
ORGANISM(S): Mus musculus 
In this work we describe the construction and application of a repurposed 3D-printer as a fraction collector. We utilise a nanoLC to ensure minimal volumes and surfaces although any LC can be coupled. The setup operates as a high-pH fractionation system capable of effectively working with nanogram s...
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD051148 | Pride
We used single-cell RNA-seq to characterize the heterogeneity of the human Lin- stromal vascular fraction across five adipose depots. We mollecularly assessed Lin- SVF cells from subcutaneous, omentum, mesocolic, and perirenal fat depots, as well as gallbladder-associated fat using 10x Genomics.
ORGANISM(S): Homo sapiens 
This study investigates how parental exposure to the water-soluble fraction (WSF) of crude oil influences transcriptomic profiles in Atlantic cod (Gadus morhua) offspring. Fish were exposed to WSF for 20 days prior to spawning, and four offspring groups were generated by fertilization: control (unex...
ORGANISM(S): Gadus morhua 
This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of...
ORGANISM(S): Homo sapiens (Human) 
2018-08-31 | PXD010315 | Pride
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