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TranscriptomicsComparison of small RNA levels in Dicer helicase mutants
Years after the discovery that Dicer is a key enzyme in gene-silencing, the role of its helicase domain remains enigmatic. Here we show that this domain is critical for accumulation of certain endogenous small interfering RNAs (endo-siRNAs) in C. elegans. The domain is required for the production of...
ORGANISM(S): Caenorhabditis elegans 
2010-04-01 | E-GEOD-20336 | biostudies-arrayexpress
TranscriptomicsTumor Cell Survival Dependence on the DExH-Box Helicase DHX9
The ATP-dependent DExH/D-box helicase DHX9 is a key participant in a number of gene regulatory steps, including transcriptional, translational, microRNA-mediated control, DNA replication, and maintenance of genomic stability. DHX9 has also been implicated in maintenance of the tumorigenic process an...
ORGANISM(S): Mus musculus 
2016-05-19 | E-GEOD-76765 | biostudies-arrayexpress
TranscriptomicsThe ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications.
To characterize the role of the ERI-6/7 helicase in endogenous small RNA pathways in C. elegans, small RNA populations from null alleles of eri-6 and eri-7, and from mutants of known endogenous RNAi pathway factors, eri-1 and ergo-1, were determined by deep sequencing, and compared to wild type. Sma...
ORGANISM(S): Caenorhabditis elegans 
2011-09-25 | E-GEOD-32366 | biostudies-arrayexpress
ProteomicsStructure of the helicase core of Werner Helicase, a key target in microsatellite instability cancers
Loss of WRN, a DNA repair helicase, was identified as a strong vulnerability of microsatellite instable(MSI) cancers, making WRN a promising drug target. We show that ATP binding and hydrolysis are required for genome integrity and viability of MSI cancer cells. We report a 2.2 Å crystal structure o...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD018910 | Pride
Helicase Hdx Deuterium exchange Dna binding 
TranscriptomicsLack of the pif1 helicase enhances zinc tolerance in S. cerevisiae
The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1D strain is tolerant to zinc overload. We demonstrate here that this effect is independent of the Pif1 helicase activity...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-31 | E-GEOD-18411 | biostudies-arrayexpress
TranscriptomicsIdentification of transcripts regulated by RNA helicase A (RHA)
This SuperSeries is composed of the following subset Series: GSE14055: Changes in polysome loading as a consequence of RHA downregulation GSE14056: Co-immunoprecipitation of RNAs with RNA helicase A (RHA) Refer to individual Series
ORGANISM(S): Chlorocebus aethiops 
2008-12-19 | E-GEOD-14057 | biostudies-arrayexpress
GenomicsMap of DnaC localization in the presence or absence of PcrA (PcrA depletion)
The replicative helicase, DnaC, was immunoprecipitated in cell extract prior to, or following conditional depletion of PcrA The differential DnaC association at each genomic location was determined before/after PcrA depletion
ORGANISM(S): Bacillus subtilis subsp. subtilis str. JH642 
2015-12-13 | E-GEOD-75953 | biostudies-arrayexpress
GenomicsGenomic Helicase-SELEX
Genome-wide discovery of natural substrates for the Rho helicase of Escherichia coli
PRJEB50170 | ENA
TranscriptomicsRNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in Helicobacter pylori
Ribonucleases (RNases) are central actors in post-transcriptional regulation, a major level of regulation of gene expression in all cells. This control plays an important role in the bacterial pathogen Helicobacter pylori, although only the function of RNase J was characterized so far. Here, we stud...
ORGANISM(S): Helicobacter pylori 
2021-06-19 | E-MTAB-10045 | biostudies-arrayexpress
GenomicsInducible Helicase-SELEX
Discovery of serotonin-inducible regulatory switches governing the activity of the Rho helicase
PRJEB50172 | ENA
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