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MetabolomicsGut Commensal Bacteria-Derived Methionine is Required for Host Reproduction by Modulating RNA m6A Methylation of the Insulin Receptor_targeted metabolites

Gut commensal bacteria promote host reproduction by modulating metabolism and nutrition, but the molecular mechanisms by which microbes regulate reproduction remain unclear. Here, we show that gut commensal bacteria promote host reproduction by providing amino-acid methionine, which controls the ...

2025-06-22 | MTBLS12470 | MetaboLights
MetabolomicsSystemic Inflammation Induces Cholestasis via Upregulating a Short Variant of FTO to Remove the m6A Methylation in the 3’-UTR of Cyp7b1 mRNAs in liver
The cholestasis frequently occurs in patients with inflammatory processes. However, the mechanisms of inflammation in inducing cholestasis are still unclear. Here, by using lipopolysaccharide (LPS)-induced cholestasis mouse models, we found that most genes encoding enzymes responsible for bile acid ...
2025-11-07 | MTBLS10713 | MetaboLights
Transcriptomicsm6a peak analysis in normal, FTO deficient and five stages of adipogenesis (D-2/0/2/5/10) in Mouse embryo fibroblast 3T3-L1 pre-adipocytes
Total RNA was extracted using TRI® Reagent (Sigma). cDNA was synthesized by RevertAid™ First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturer’s recommendations. PCR reactions were carried out on a DNAEngine® Thermal Cycler (PTC-0200G, Bio-Rad) in 25 μl reacti...
ORGANISM(S): Mus musculus 
2015-03-09 | E-GEOD-53244 | biostudies-arrayexpress
GenomicsProfiling of mRNA N6-Adenosine Methylation (m6A) in Arabidopsis
m6A profiling in two accessions of Arabidopsis thaliana (Can-0 and Hen-16) using the m6A-targeted antibody coupled with high-throughput sequencing m6A-seq in two accessions of Arabidopsis, two replicates for each sample
ORGANISM(S): Arabidopsis thaliana 
2014-11-06 | E-GEOD-59154 | biostudies-arrayexpress
Transcriptomicsm6A mapping in human RNA (untreated)
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Identification of m6A modified sequences in HepG2 cells.
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-37003 | biostudies-arrayexpress
Transcriptomicsm6A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency
N6-methyladenosine (m6A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m6A modification profiles in the mRNA transcriptomes of four cell...
ORGANISM(S): Mus musculus 
2015-03-10 | E-GEOD-52125 | biostudies-arrayexpress
TranscriptomicsYeast m6A methylated mRNAs are enriched on translating ribosomes during meiosis, and under Rapamycin treatment
Interest in mRNA methylation has exploded in recent years. The sudden interest in a 40 year old discovery was due in part to the recent finding of FTO?s (Fat Mass Obesity) N6-methyl-adenosine (m6A) deaminase activity, thus suggested a link between obesity-associated diseases and the presence of m6A...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2015-08-12 | E-GEOD-68435 | biostudies-arrayexpress
TranscriptomicsPlacental epitranscriptome-wide analysis of revealed the m6A modification level at 5’ UTR related fetal growth
To further understand the relationship between the level of gene expression and protein translation in human placenta, we focused on N6-methyladenosine: m6A, which is a methylation modification of mRNA acting as a post-transcriptional regulation which has drawn attention in recent years. It is said ...
ORGANISM(S): Homo sapiens 
2019-10-10 | E-MTAB-6507 | biostudies-arrayexpress
Transcriptomicsm6A mapping in mouse RNA (mouse liver and human brain)
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing Identification of m6A modified sequences in mouse liver and human brain
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-37004 | biostudies-arrayexpress
TranscriptomicsTopology of the human and mouse m6A RNA methylomes revealed by m6A-seq
This SuperSeries is composed of the following subset Series: GSE36958: Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions GSE37001: METTL3 KD in HepG2 cells GSE37002: m6A mapping in human RNA (with treatments) GSE37003: m6A mapping in huma...
ORGANISM(S): Saccharomyces cerevisiae 
2012-04-24 | E-GEOD-37005 | biostudies-arrayexpress
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