M6A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency

ABSTRACT: N6-methyladenosine (m6A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m6A modification profiles in the mRNA transcriptomes of four cell types with different degrees of pluripotency. Comparative analysis reveals several features of m6A, especially gene- and cell-type-specific m6A mRNA modifications. We also show that microRNAs (miRNAs) regulate m6A modification via a sequence pairing mechanism. Manipulation of miRNA expression or sequences alters m6A modification levels through modulating the binding of METTL3 methyltransferase to mRNAs containing miRNA targeting sites. Increased m6A abundance promotes the reprogramming of mouse

ORGANISM(S): Mus musculus

SUBMITTER: Xiu-Jie Wang 

PROVIDER: E-GEOD-52125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress