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ProteomicsPhosphoproteomics of Matrigel-embedded samples
Phosphoproteomics data of Matrigel-embedded HCT116 spheroid, and patient-derived colorecral cancer organoid
ORGANISM(S): Homo Sapiens (human) 
2019-02-24 | PXD009032 | JPOST Repository
ProteomicsTissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids
Matrigel, a mouse tumor extracellular matrix (ECM) protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to batch variation, high cost, and...
ORGANISM(S): Sus scrofa (Pig) Mus musculus (Mouse) 
2022-02-10 | PXD023694 | Pride
Organoid Matrigel Lc-ms/ms 
ProteomicsThe influence of different Matrigel dissolving methods on proteome research of human derived organoids
Here we investigated the influence of different Matrigel dissolving methods on organoid proteomics.
ORGANISM(S): Homo Sapiens Mus Musculus 
PXD029173 |
TranscriptomicsEpithelium-only cultured stem cells isolated from human pluripotent stem cell derived intestinal organoids grown in matrigel and alginate
Human intestinal organoids were grown in a typical 3D matrigel culture environment, or in an alginate gel, then a subset from each condition were xenotransplanted to vascularized and mature in vivo. Epithelium was isolated and epithelial only organoids (enteroids) were then grown from each condition...
ORGANISM(S): Homo sapiens 
2019-06-27 | E-MTAB-7000 | biostudies-arrayexpress
TranscriptomicsExpression analysis of HepG2, HepG2-slug and HepG2-slug on Matrigel
Investigation of whole genome gene expression level changes in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Whole genome gene expression level changes have been compared in hepatocellular carcinoma cell line hepG2 in regular c...
ORGANISM(S): Homo sapiens 
2012-09-21 | E-GEOD-41026 | biostudies-arrayexpress
ProteomicsProteomics of Matrigel, LC-MS/MS
ECM is the physicochemical support for the cells living in the tissue microenvironment. it is important to know the protein contents within the ECM that are critical for extra- and intracellular signaling, cell growth, migration, cell-cell/ECM interactions. The ECM gel derived from the Engelbreth-H...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-26 | PXD005696 | Pride
Ecm Tms Matrigel Tissue matrix scaffold Lc-ms/ms 
TranscriptomicsTranscription profiling of human liver LI90 cells cultured on Matrigel
Transcriptional analysis of human hepatic stellate cells cultured on Matrigel.
ORGANISM(S): Homo sapiens 
2010-03-01 | E-MEXP-2582 | biostudies-arrayexpress
TranscriptomicsHUVEC with/without VEGF-A treatment in 3D matrigel or 2D monoculture
To profile changes in gene expression in human endothelial cells in response to VEGF-A165 and phenotypic changes during vascular network formation in vitro 16 samples of human umbilical vein endothelial cells were analysized, four distinct biological samples were used in each condition. The four con...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-53550 | biostudies-arrayexpress
TranscriptomicsRNA-seq of wildtype mouse small intestinal organoids grown in Matrigel or in/on collagen.
We aimed to analyse the effect of different extra-cellular matrices on the growth of small intestinal organoids. Small intestinal crypts of wildtype mice were harvested and grown under standard Matrigel organoid conditions. After establishment of organoids (passaged 1-2), organoids were grown in Ma...
ORGANISM(S): Mus musculus 
2021-04-01 | E-MTAB-10082 | biostudies-arrayexpress
TranscriptomicsGene expression profile of human embryonic stem cell-derived hepatocyte-like cells in matrigel and recombinant laminins
Stem cell derived somatic cells represent an unlimited resource for basic and translational science. While promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte. Current protocols produce variable pop...
ORGANISM(S): Homo sapiens 
2016-01-18 | E-MTAB-3994 | biostudies-arrayexpress
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