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ProteomicsMultiplexed complexome profiling using tandem mass tags
Complexome profiling is a rapidly spreading, powerful approach to gain insight into the nature of protein complexes. It identifies and quantifies protein complexes separated into multiple fractions of increasing molecular mass using mass spectrometry-based, label-free bottom-up proteomics. Complexom...
ORGANISM(S): Homo sapiens (Human) 
2021-08-20 | PXD023211 | Pride
Complexome profiling  multiplexed complexomics  tandem mass tags  protein complexes 
ProteomicsAutomated sample preparation workflow for tandem mass tag-based proteomics
Although tandem mass tag (TMT)-based isobaric labeling has become a powerful technique for multiplexed protein quantitation, it has not been easy to automate the workflow for widespread adoption. This is because preparation of TMT labeled peptide samples involves multiple steps ranging from protein ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040788 | Pride
Automation Tandem mass tags Proteomics 
UnknownResin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation.
Not available
S-EPMC8828046 | biostudies-literature
UnknownAutomated Sample Preparation Workflow for Tandem Mass Tag-Based Proteomics.
Not available
S-EPMC10557128 | biostudies-literature
ProteomicsReproducible Workflow for Multiplexed Deep-Scale Proteome and Phosphoproteome Analysis of Tumor Tissues by Liquid Chromatography-Mass Spectrometry

Mertins P, Tang LC, Krug K, Clark DJ, Gritsenko MA, Chen L, Clauser KR, Clauss TR, Shah P, Gillette MA, et al., Nat Protoc. 2018 Jul 9. doi: 10.1038/s41596-018-0006-9

Here we present an optimized workflow for gl...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-08-30 | MSV000084265 | MassIVE
UnknownAssessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling.
Not available
S-EPMC7357674 | biostudies-literature
ProteomicsLimits for resolving isobaric tandem mass tag reporter ions using phase constrained spectrum deconvolution
A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT) which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-05 | PXD011009 | Pride
Q exactive hf-x Phase constrained spectrum deconvolution Tmt Resolution Quantitative proteomics 
ProteomicsTMT-based Multiplexed (Chemo)proteomics on the Orbitrap Astral Mass Spectrometer
This dataset contains an evaluation of TMT-based multiplexed proteomics on the Orbitrap Astral mass spectrometer.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-17 | MSV000096674 | MassIVE
UnknownIn-depth proteome analysis of brain tissue from Ewsr1 knockout mouse by multiplexed isobaric tandem mass tag labeling.
Not available
S-EPMC10502055 | biostudies-literature
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
PXD025536 | JPOST Repository
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