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MetabolomicsTargeting SLC1A5-mediated glutamine metabolism overcomes PARP inhibitor resistance in ovarian cancer

Poly(ADP-ribose) polymerase inhibitors (PARPi), such as olaparib, have significantly improved outcomes in ovarian cancer (OC); however, therapy resistance remains a major clinical challenge. Here, we showed that glutamine metabolism was upregulated in PARPi-resistant OC cells and identified the g...

2026-04-08 | MTBLS14072 | MetaboLights
TranscriptomicsPARP-1, H3 and H3K4me3 ChIP-chip from MCF7 cells
We are examining the mechanisms by which the nuclear enzyme PARP-1 regulates gene expression. We performed ChIP-chip for PARP-1, histone H3 and H3K4me3 (lysine 4 trimethylation on histone H3) in MCF7 breast carcinoma cells, and looked at 23550 RefSeq genes to determine binding patterns of these fac...
ORGANISM(S): Homo sapiens 
2010-09-20 | E-GEOD-19619 | biostudies-arrayexpress
TranscriptomicsRNAi of human MCF7 cells knocked down for PARP-1 or PARGs
Poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG) are enzymes that modify target proteins in the nucleus by the addition and removal, respectively, of ADP-ribose polymers. Although a role for PARP-1 in gene regulation has been well established, the role of PARG is le...
ORGANISM(S): Homo sapiens 
2009-10-30 | E-GEOD-12952 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Parp-1 deficient and wild type T cells following CD3 and CD3/CD28 activation
Primary T cells were isolated from spleen of Parp-1-/- and wild-type mice by magnetic depletion of non-T cells using a MACS Pan-T Cell isolation kit, according to the manufacturer´s instruction (Mintenyi Biotec, Bergisch Gladbach, Germany). Purity was assessed by flow cytometry analysis using antibo...
ORGANISM(S): Mus musculus 
2008-08-04 | E-MEXP-1237 | biostudies-arrayexpress
MetabolomicsMetabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts (Part 2)
Not available
ORGANISM(S): Mus musculus 
ST000275 | MetabolomicsWorkbench
MetabolomicsMetabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts (Part 3)
Not available
ORGANISM(S): Mus musculus 
ST000183 | MetabolomicsWorkbench
TranscriptomicsExamination of functional interplay between NMNAT-1 (a nuclear NAD+ synthase) and PARP-1 (a nuclear poly(ADP-ribose) polymerase)
NMNAT-1 and PARP-1, two key enzymes in the NAD+ metabolic pathway, localize to the nucleus where integration of their enzymatic activities has the potential to control a variety of nuclear processes. Using a variety of biochemical, molecular, cell-based, and genomic assays, we show that NMNAT-1 and...
ORGANISM(S): Homo sapiens 
2012-02-15 | E-GEOD-35358 | biostudies-arrayexpress
GenomicsEssential role of poly(ADP-ribosyl)ation in cocaine action
Many of the long-term effects of cocaine on the brain's reward circuitry have been shown to be mediated by alterations in gene expression. Several chromatin modifications, including histone acetylation and methylation, have been implicated in this regulation, but the effect of other histone modifica...
ORGANISM(S): Mus musculus 
2014-02-20 | E-GEOD-55136 | biostudies-arrayexpress
TranscriptomicsRNA-seq from control and macroH2A1-depleted IMR90 primary human lung fibroblasts
The histone variant macroH2A1 and the poly(ADP-ribose) polymerase PARP-1 both regulate gene transcription by modulating chromatin structure and function. Of the two macroH2A1 splice variants, macroH2A1.1 and macroH2A1.2, the former is often suppressed in cancer and has the unique ability to interact...
ORGANISM(S): Homo sapiens 
2014-09-15 | E-GEOD-54846 | biostudies-arrayexpress
ProteomicsOlaparib based photo-affinity probes for PARP-1 detection in living cells
The poly-ADP-ribose polymerase (PARP) is a protein from the family of ADP-ribosyltransferases that catalyzes poly adenosine diphosphate ribose (ADPR) formation in order to attract the DNA repair machinery to DNA damage sites. Inhibition of PARP activity by olaparib can cause cell death which is of c...
ORGANISM(S): Homo sapiens (Human) 
2020-04-29 | PXD018661 | Pride
Olaparib  parp-1  bioorthogonal chemistry  chemical proteomics  photoaffinity probes 
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