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ProteomicsPhenotypic Proteomic Profiling Reveals Convergent Kinase Targets for Circadian Clock Modulators
Determining the mechanisms of action of drug molecules that modulate circadian rhythms is critical to develop novel compounds to treat clock disorders. Here we employed Phenotypic Proteomic Profiling (PPP) integrating multipronged proteomics approaches including global proteome, phosphoproteome, kin...
ORGANISM(S): Homo sapiens (Human) 
2019-11-25 | PXD014186 | Pride
Circadian rhythms  circadian period altering compounds  kinase  phenotypic proteome profiling  phosphoproteome  proteomics  thermal proteome profiling 
ProteomicsIntegrated Phenotypic and Activity-Based Profiling of Electrophilic Fragments Reveals New Targets for Antibiotics Development
The emergence of antibiotic resistance necessitates the discovery of novel bacterial targets and antimicrobial agents. Here, we present a bacterial target discovery framework that integrates phenotypic screening of cysteine-reactive fragments with competitive activity-based protein profiling to map ...
ORGANISM(S): Shigella flexneri S5644 Bacteria 
2025-05-07 | PXD058002 | Pride
Phenotypic screening  covalent inhibitor  antibiotics  activity-based protein profiling  fabh  miaa  pdxy 
UnknownComparison of Quantitative Mass Spectrometric Methods for Drug Target Identification by Thermal Proteome Profiling.
Not available
S-EPMC10407934 | biostudies-literature
ProteomicsProteomic workflows for deep phenotypic profiling of 3D organotypic liver models
Organotypic human tissue models constitute promising systems to facilitate drug discovery and development. They allow to maintain native cellular phenotypes and functions, which enables long-term pharmacokinetic and toxicity studies, as well as phenotypic screening. To trace relevant phenotypic chan...
ORGANISM(S): Homo sapiens (Human) 
2024-04-29 | PXD047462 | Pride
Proteomics Hepatocyte Mass spectrometry Organoid 
GenomicsTemperature sensitive Mutant Proteome Profiling: a novel tool for the characterization of the global impacts of missense mutants on the proteome
Temperature sensitive Mutant Proteome Profiling: a novel tool for the characterization of the global impacts of missense mutants on the proteome
PRJNA599371 | ENA
UnknownAneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast.
Not available
S-EPMC2978756 | biostudies-literature
TranscriptomicsTemperature sensitive Mutant Proteome Profiling: a novel tool for the characterization of the global impacts of missense mutants on the proteome
RNA-seq experiments measuring global RNA abundance Temperature sensitive (TS) mutants are a tool that have been foundational for the study of many essential life processes. Despite the long-term use of TS mutants, the mechanisms that lead to temperature sensitivity are not fully understood. Furtherm...
ORGANISM(S): Saccharomyces cerevisiae 
2020-01-08 | GSE143236 | GEO
UnknownThermal Proteome Profiling to Identify Protein-ligand Interactions in the Apicomplexan Parasite Toxoplasma gondii.
Not available
S-EPMC8595413 | biostudies-literature
ProteomicsAssessment of the nematocidal potency of ABX464 analogues and target deconvolution in Caenorhabditis elegans by thermal proteome profiling
Global challenges with anthelmintic failure and resistance development lends impetus to the development of new nematocides (anthelmintics) with novel mechanism(s) of action. The free-living nematode Caenorhabditis elegans is as an important model organism used for drug discovery and a powerful tool...
ORGANISM(S): Caenorhabditis elegans 
2024-01-29 | PXD042930 | Pride
Thermal proteome profiling  lc-ms/ms  caenorhabditis elegans 
ProteomicsExploring the mode of action of a novel antileishmanial compound using thermal proteome profiling and other OMICs tools
Compound 4, an antileishmanial compound identified in a GSK phenotypic-screen against Leishmania donovani (Leish-box)1 and not currently being actively researched for drug discovery, was investigated with the aim of elucidated its mode-of-action (MoA) in L. major and L. mexicana (two causative agent...
ORGANISM(S): Leishmania mexicana Leishmania major 
2025-05-10 | PXD063779 | Pride
Omics Thermal proteome profiling Leishmania Mode of action 
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