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ProteomicsCharacterization of human sperm protamine proteoforms through a combination of top-down and bottom-up mass spectrometry approaches
Protamine 1 (P1) and protamine 2 family (P2) are the most abundant nuclear basic spermspecific proteins, packing 85-95% of the paternal DNA. P1 is synthesized as a mature form, whereas P2 components (HP2, HP3 and HP4) arise from the proteolysis of the precursor (pre-P2). Due to the particular protam...
ORGANISM(S): Homo sapiens (Human) 
2019-11-11 | PXD014618 | Pride
Protamines  sperm  post-translational modifications  phosphorylation  nonannotated proteins  male infertility. Top-down proteomics  bottom-up proteomics  proteoforms  electron transfer dissociation 
ProteomicsProtamine characterization by top-down proteomics: Boosting proteoform identification with DBSCAN
Protamines replace histones as the main nuclear protein in sperm cells having a crucial role in compacting the paternal genome. Human spermatozoa contain the protamine 1 (P1) and the family of protamine 2 (P2) proteins. Alterations in protamine PTMs or on the P1/P2 ratio could be associated with mal...
ORGANISM(S): Homo sapiens (Human) 
2021-05-13 | PXD024405 | Pride
Male infertility  sperm  protamine  lc-ms/ms  top down proteomics  proteoform  post-translational modifications  bioinformatics  dbscan. 
ProteomicsMouse sperm protamine mass spectrometry
Conventional dogma presumes that protamine-mediated DNA compaction in sperm is achieved by passive electrostatics between DNA and the arginine-rich core of protamines. However, phylogenetic analysis reveals several non-arginine residues that are conserved within, but not across, species. The functio...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-17 | PXD028917 | Pride
Sperm Protamine Chromatin 
ProteomicsAlterations in the abundance of protamine proteoforms related to sperm chromatin packaging, obesity, and age in men with unexplained infertility
Protamine1 (P1) and Protamine 2 family (P2) are the most relevant nuclear proteins in the male gamete since are necessary to compact and protect the paternal genome. Human and mouse sperm contain a complex population of protamine proteoforms, which raises the interest to decipher whether it responds...
ORGANISM(S): Homo sapiens (Human) 
2025-04-25 | PXD049367 | Pride
Proteoforms Top-down proteomics Protamines Male infertility Sperm Chromatin 
ProteomicsIn-depth investigation of low-abundant proteins in matured and filling stages seeds of Glycine max by a combination of protamine sulfate precipitation and TMT-based quantitative proteomic analysis
The presence of seed storage proteins is a major bottleneck in soybean seed proteome analysis despite the significant technical advancements in mass spectrometry and data analysis software. Our previous studies have shown that effective ways to improve the detection of low-abundance proteins in soyb...
ORGANISM(S): Glycine max 
2020-06-28 | PXD019155 | Pride
Low-abundant proteins Perseus Tandem mass tags Basic ph reversed phase Maxquant Filter-aided sample preparation Protamine sulfate precipitation Glycine max 
GenomicsDistinctive Chromatin in Human Sperm Packages Genes that Guide Embryo Development: ChIP-seq
As nucleosomes are widely replaced by protamine in mature human sperm, epigenetic contributions to embryo development appear limited. However, our genome-wide approaches find nucleosomes at low levels genome-wide, but also significantly enriched at imprinted gene clusters, miRNA clusters, HOX gene...
ORGANISM(S): Homo sapiens 
2009-06-15 | E-GEOD-15690 | biostudies-arrayexpress
GenomicsPHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
PRJNA563578 | ENA
ProteomicsASB9 promotes ubiquitin-mediated degradation of TNP2 to facilitate histone-to-protamine transition in humans and mice
During spermiogenesis, nuclear remodeling occurs where histones are initially replaced by transition proteins (TNPs) and subsequently by protamines, a process essential for sperm maturation. Although the degradation of histones and TNPs is thought to be essential for sperm nuclear remodeling, the un...
ORGANISM(S): Mus Musculus 
2025-11-19 | PXD070974 |
ProteomicsFam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis
Families with sequence similarity 170 members A (Fam170a) deficiency caused abnormal sperm nuclear morphology and male infertility in mice, mirroring the observation of very low Fam170a transcription in sperm of infertile men with teratozoospermia.Fam170a directly interacted with the deubiquitinatin...
ORGANISM(S): Mus Musculus 
2024-11-10 | PXD057721 |
GenomicsPhase-separated CCER1 coordinates histone-to-protamine transition and fertility
Phase-separated CCER1 coordinates histone-to-protamine transition and fertility
PRJNA877103 | ENA
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