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UnknownImprovements in proteomic metrics of low abundance proteins through proteome equalization using ProteoMiner prior to MudPIT.
Not available
S-EPMC3161494 | biostudies-literature
ProteomicsEvaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Neurological Disorders
This study evaluated and compared three sample preparation strategies for proteomic analysis of equine cerebrospinal fluid (CSF): native in-solution digestion, the ProteoMiner™ Small-Capacity Enrichment Kit (Bio-Rad®), and the PreOmics Enrich-iST™ Kit (PreOmics®). CSF is in direct contact with the c...
ORGANISM(S): Equus caballus (Horse) 
2026-04-13 | PXD063239 | Pride
Cerebrospinal fluid (csf)  proteomics  native digestion  proteominer™ small-capacity enrichment kit  preomics enrich-ist™  liquid chromatography with tandem mass spectrometry (lc-ms/ms) 
ProteomicsThe exploration of Missing Proteins by a combination approach to enrich the low abundance membrane proteins from several cancer cell lines
Our goal is the identification of more and more the missing proteins from cancer cell lines. This project focuses on the membrance proteins separated by the ultracentrifuge and the low abundant peptides enriched by the Proteominer Kit and the hydrophobic peptides extracted by the organic solvent Ace...
ORGANISM(S): Homo sapiens (Human) 
2019-12-06 | PXD014058 | Pride
Proteomics Cancer cell lines Missing protein Lc-ms/ms 
ProteomicsAffinity capture enrichment versus Affinity depletion: A comparison of strategies for increasing coverage of low abundant human plasma proteins
present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from plasma samples thereb...
ORGANISM(S): Homo sapiens (Human) 
2020-08-27 | PXD020727 | Pride
Affinity enrichment Depletion Human plasma 
ProteomicsDDA-MS serum proteomics in dogs with leishmaniosis
Serum proteomes of healthy and Leishmania-infected dogs were analyzed by DDA-MS approach to generate sample-specific spectral library for subsequent SWATH-MS analysis, namely pooled, mixed control, mixed case (Leishmania-infected), ProteoMiner enriched, and 3 SCX fractions; all containing iRT pepti...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2023-05-10 | PXD039763 | Pride
Canine Proteomics Serum Leishmania infantum Dda 
ProteomicsEquine synovial fluid
The aim of the study was to characterise the protein complement of synovial fluid (SF) in health and osteoarthritis (OA) using liquid chromatography mass spectrometry (LC-MS/MS) following peptide-based depletion of high abundance proteins. SF was used from nine normal and nine OA Thoroughbred hors...
ORGANISM(S): Equus caballus (Horse) 
2022-03-02 | PXD001509 | Pride
Osteoarthritis Synovial fluid Equine 
ProteomicsThe exploration of Missing Proteins by a combination approach to enrich the low abundance membrane proteins from several cancer cell lines
Our goal is the identification of more and more the missing proteins from cancer cell lines. This project focuses on the membrance proteins separated by the ultracentrifuge and the low abundant peptides enriched by the Proteominer Kit and the hydrophobic peptides extracted by the organic solvent Ace...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-26 | MSV000086369 | MassIVE
ProteomicsDistinct Serum Proteome Profiles Associated with Collagen-induced Arthritis, and Complete Freund’s Adjuvant-induced Inflammation in CD38-/- mice: the Discriminative Power of Protein Species or Proteoforms. Spot 269_Spot 500
Collagen-type-II-induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA in C57BL/6 mice resembles human rheumatoid arthritis (RA) in terms of its disease course, histological findings, and in its response...
ORGANISM(S): Mus musculus (Mouse) 
2015-07-17 | PXD002071 | Pride
Proteoforms Inflammation Cd38 Arthritis Low-abundance proteins Protein species 
ProteomicsDistinct Serum Proteome Profiles Associated with Collageninduced Arthritis, and Complete Freund’s Adjuvant-induced Inflammation in CD38-/- mice: the Discriminative Power of Protein Species or Proteoforms
Collagen-type-II-induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA in C57BL/6 mice resembles human rheumatoid arthritis (RA) in terms of its disease course, histological findings, and in its respons...
ORGANISM(S): Mus musculus (Mouse) 
2015-07-17 | PXD001788 | Pride
Proteoforms Cd38 Inflammation Arthritis Low-abundance proteins Protein species 2d-dige 
UnknownAffinity depletion versus relative protein enrichment: a side-by-side comparison of two major strategies for increasing human cerebrospinal fluid proteome coverage.
Not available
S-EPMC6390343 | biostudies-literature
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