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Transcriptomics3' mRNA-seq of protrusions and cell bodies of BJ, PC-3M, RPE-1, U-87 and WM-266.4 cells
3' mRNA-seq of protrusions and cell bodies of a panel of normal and malignant cell lines. These data show that Ribosomal protein (RP) -mRNAs localization to cell protrusions is a conserved phenomenon.
ORGANISM(S): Homo sapiens 
2020-10-30 | E-MTAB-8470 | biostudies-arrayexpress
TranscriptomicsQUANTSEQ 3' sequencing of protrusions and cell bodies of control (NT) or LARP6 siRNA (LARP6 KD) transfected MDA-MB231 breast cancer cells
To assess how LARP6 affects mRNA localisation to cell-protrusions, we transfected MDA-MB231 cells with either non-targeting control siRNA or LARP6 siRNA for 72 hrs, before fractionating the cells into protrusion and cell-bodies, and analysed total RNA from each fraction via Lexogen QUANTSEQ FWD 3' m...
ORGANISM(S): Homo sapiens 
2020-10-30 | E-MTAB-9520 | biostudies-arrayexpress
Transcriptomics3' mRNA-seq of protrusions and cell bodies of non-transfected (nt) or LARP6 siRNA transfected MDA-MB231 cells
3' mRNA-seq of protrusions and cell bodies of nt or LARP6 siRNA transfected MDA-MB231 cells. These data show that Ribosomal protein (RP) -mRNAs localization to cell protrusions is mediated by LARP6.
ORGANISM(S): Homo sapiens 
2022-10-03 | E-MTAB-8472 | biostudies-arrayexpress
GenomicsIdentification of mRNAS enriched in endothelial cell protrusions
Identification of mRNAS enriched in endothelial cell protrusions
PRJNA549849 | ENA
GenomicsRNA localization to protrusions of mouse melanoma YUMM1.7 cells.
RNA localization to protrusions of mouse melanoma YUMM1.7 cells.
PRJNA907013 | ENA
ProteomicsProtrusion vs Cell-body SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo sapiens (Human) 
2015-10-09 | PXD000914 | Pride
Human silac proteomics protrusion lc-ms/ms orbitrap-velos maxquant 
TranscriptomicsLocalized Rho GTPase Activation Regulates RNA Dynamics and Compartmentalization in Tumor Cell Protrusions
mRNA trafficking and local protein translation are associated with protrusive cellular domains, such as neuronal growth cones, and deregulated control of protein translation is associated with tumor malignancy. We show here that activated RhoA, but not Rac1, is enriched in pseudopodia of MSV-MDCK-IN...
ORGANISM(S): Canis lupus familiaris 
2010-05-18 | E-GEOD-13253 | biostudies-arrayexpress
TranscriptomicsRNA-seq of coding RNAs from protrusions of MDA-MB231 cells treated with control or exosome core complex siRNA
MDA-MB-231 breast carcinoma cells were treated by non-targetting siRNA or siRNAs against EXOSC8. Subsequently, mRNAs from protrusions of the cells were quantified by RNA-seq in presence of Rho-kinase inhibitor H1152, using a filter based fractionation method.
ORGANISM(S): Homo sapiens 
2016-07-03 | E-MTAB-2548 | biostudies-arrayexpress
ProteomicsFam49/CYRI interacts with Rac1 and locally suppresses protrusions
Actin-based protrusions driving cell migration are reinforced through positive feedback, but it is unclear how the cell restricts the eventual size of protrusions, or limits positive signals to allow them to split or retract. We have identified an evolutionarily conserved regulator of the protrusio...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2018-10-08 | PXD010460 | Pride
Lamellipodia Cyfip-related rac interactor Dictiostelium discoideus Gfp-trap with formaldehyde crosslinking Scar/wave complex Cell migration 
ProteomicsTMT quantitative proteomics of a) protrusions and cells bodies of a panel of cell lines, b) protrusions and cells bodies of MDA-MB231 cells collected after 1 h, 2 h, 4 h, 8 h post- induction of protrusions, c) non-transfected (nt) or LARP6 siRNAs MDA-MB231 cells grown on either close or open collagen coated transwells and d) protrusions and cells bodies of a panel of breast cell lines.
Translation of Ribosomal Protein coding mRNAs (RP-mRNAs) constitutes a key step in regulation of ribosome biogenesis in human cells, but the exact mechanisms which modulate RP-mRNAs translation under various cellular and environmental conditions remain poorly understood. Here we show that the subcel...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD015808 | Pride
Human Malignant and non-malignant cell lines Lc-ms/ms 
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