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MetabolomicsGlyceraldehyde-3-phosphate dehydrogenase homologs as bifunctional gatekeepers of metabolic segregation in Pseudomonas putida

Metabolically versatile Pseudomonas species can assimilate various glycolytic and gluconeogenic substrates. Simultaneous assimilation is known to involve segregating carbons from each substrate type into different metabolic pathways, but the mechanisms that govern this segregation remain unresolv...

2025-11-03 | MTBLS12468 | MetaboLights
MetabolomicsMetabolomics analysis reveals the participation of efflux pumps and ornithine in the response of Pseudomonas putida DOT-T1E cells to challenge with propranolol
Efflux pumps are critically important membrane components that play a crucial role in strain tolerance in Pseudomonas putida to antibiotics and aromatic hydrocarbons that result in these toxicants being expelled from the bacteria. Here, the effect of propranolol on P. putida was examined by sudden a...
2016-08-23 | MTBLS320 | MetaboLights
MetabolomicsMetabolic analysis of the response of Pseudomonas putida DOT-T1E strains to toluene using Fourier transform infrared spectroscopy and gas chromatography mass spectrometry
To elucidate any observable metabolic alterations during interactions of several strains of Pseudomonas putida (DOT-T1E, and its mutants DOT-T1E-PS28 and DOT-T1E-18) with the aromatic hydrocarbon toluene, metabolomic approaches were employed. Initially, Fourier-transform infrared (FT-IR) spectroscop...
2016-08-23 | MTBLS319 | MetaboLights
Transcriptomics3CB-induced gene expression of Pseudomonas putida KT2440/pSL1 in soil (with RNA amplification)
Genome-wide scanning of gene expression by microarray techniques was successfully performed on RNA extracted from a sterilized soil inoculated with Pseudomonas putida KT2440/pSL1, which contains a chloroaromatic degrading plasmid, in the presence or absence of 3-chlorobenzoic acid (3CB). The genes s...
ORGANISM(S): Pseudomonas putida KT2440 
2011-03-29 | E-GEOD-28215 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Pseudomonas putida PP3546 mutant
Transcription profiling of Pseudomonas putida PP3546 mutant
ORGANISM(S): Pseudomonas putida 
2013-04-02 | E-MEXP-3655 | biostudies-arrayexpress
TranscriptomicsExpression data from Pseudomonas putida KT2440 (RNA-Seq data)
We used cDNA generated from total mRNA for RNA-Seq analysis (Genome Analyzer II at GATC Biotech AG) to monitore the gene expression of P. putida after a cold shock from 30M-BM-0C to 10M-BM-0C Culture was grown in minimal medium supplemented with succinate at 30M-BM-0C. In mid-exp. phase cells were ...
ORGANISM(S): Pseudomonas putida KT2440 
2011-02-01 | E-GEOD-24175 | biostudies-arrayexpress
TranscriptomicsThe IHF regulon of exponentially growing Pseudomonas putida cells
Integration host factor (IHF) sites are largely absent from intergenic regions of ORFs encoding central metabolic functions in Pseudomonas putida mt-2. To gain an insight into this unequal distribution of otherwise abundant IHF-binding sequences, the transcriptome of IHF-plus and IHF-minus cells gro...
ORGANISM(S): Pseudomonas putida 
2012-07-24 | E-GEOD-23499 | biostudies-arrayexpress
TranscriptomicsKT2440 vs KT2440_NAH7
Plasmid-free Pseudomonas putida KT2440 compared with the same strain harbouring NAH7 plasmid; all the cells were grown in minimal medium M9 with glucose
ORGANISM(S): Pseudomonas putida 
2012-07-27 | E-MEXP-3412 | biostudies-arrayexpress
TranscriptomicsPseudomonas putida KT2440 cells: control vs. 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)
Transcriptome profiling of Pseudomonas putida KT2440 comparing cells exposed for 1 hour to DIMBOA from maize (Zea mays) to unexposed cells Two-condition experiment, unexposed vs. exposed, three biological replicates independantly grown and harvested. A dye swap was performed on each biological repli...
ORGANISM(S): Pseudomonas putida KT2440 
2012-07-08 | E-GEOD-36489 | biostudies-arrayexpress
ProteomicsOuter membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440
Lignin modifications and the exoproteome of three aromatic catabolic bacteria: Pseudomonas putida KT2440, Rhodoccocus jostii RHA1, and Amycolatopsis sp. ATCC 39116. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MSGF+ and abundances inferred using PNNL's DMS an...
ORGANISM(S): Pseudomonas Putida Kt2440 (ncbitaxon:160488) Amycolatopsis Sp. Atcc 39116 (ncbitaxon:385957) Rhodococcus Jostii Rha1 (ncbitaxon:101510) 
2019-11-01 | MSV000084524 | MassIVE
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