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TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
GenomicsTranslational profiling of zebrafish retina cells using translating ribosome affinity purification methods
Translational profiling of zebrafish retina cells using translating ribosome affinity purification methods
PRJNA791684 | ENA
GenomicsVirus purification methods for metagenomes
Evaluation of methods to purify virus-like particles for the sequencing of intestinal viromes
PRJEB6941 | ENA
TranscriptomicsEvaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling
RNA-Seq technique was applied to investigate the effects of two semen collection methods (Pelleted vs Liquefied) and two sperm purification methods (SCLB vs PS) to the integrity of isolated RNAs at different perspectives. The same set of semen samples were applied to investigate the qualitative and ...
ORGANISM(S): Homo sapiens 
2013-12-09 | E-GEOD-43586 | biostudies-arrayexpress
TranscriptomicsThe Extracellular RNA Quality Control (exRNAQC) study: Small RNA sequencing of RNA purification kit study (exRNAQC011)
To evaluate the impact of the RNA purification method on extracellular RNA (exRNA) sequencing, 8 different RNA purification kits were compared by applying Small RNA sequencing (Illumina) to exRNA from human healthy donor plasma. Minimum and maximum plasma input volumes recommended by the manufacture...
ORGANISM(S): Homo sapiens 
2021-06-10 | E-MTAB-10505 | biostudies-arrayexpress
GenomicsBenchmark study of different chromatin isolation methods using the E2Fa transcription factor
Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation (ChIP), this method has some inhere...
ORGANISM(S): Arabidopsis thaliana 
2014-02-04 | E-GEOD-53422 | biostudies-arrayexpress
TranscriptomicsEvaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling
RNA-Seq technique was applied to investigate the effects of two semen collection methods (Pelleted vs Liquefied) and two sperm purification methods (SCLB vs PS) to the integrity of isolated RNAs at different perspectives.
ORGANISM(S): Homo sapiens 
2013-12-09 | GSE43586 | GEO
TranscriptomicsThe Extracellular RNA Quality Control (exRNAQC) study: Small RNA sequencing of interaction study (exRNAQC017)
Study to detect possible interactions between blood collection tubes and RNA purification methods on extracellular RNA (exRNA) sequencing. Different combinations of blood collection tubes (3 in total), time intervals between blood draw and downstream processing (immediate (T0), after 4 hours (T4), a...
ORGANISM(S): Homo sapiens 
2022-12-25 | E-MTAB-12542 | biostudies-arrayexpress
TranscriptomicsThe Extracellular RNA Quality Control (exRNAQC) study: RNA Exome sequencing of interaction study (exRNAQC017)
Study to detect possible interactions between blood collection tubes and RNA purification methods on extracellular RNA (exRNA) sequencing. Different combinations of blood collection tubes (3 in total), time intervals between blood draw and downstream processing (immediate (T0), after 4 hours (T4), a...
ORGANISM(S): Homo sapiens 
2022-12-25 | E-MTAB-12541 | biostudies-arrayexpress
TranscriptomicsTranslational profiling of zebrafish retina cells using translating ribosome affinity purification methods
Translating ribosome affinity purification (TRAP) methods allow cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. In this study, we purified and analysed the polyribosome-associated RNA fraction of zebrafish embryo´s retinas at 22 ho...
ORGANISM(S): Danio rerio 
2021-12-29 | GSE192511 | GEO
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