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MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
TranscriptomicsB-Raf inhibitors induce epithelial differentiation in BRAF mutant colorectal cancer cells
Mutations in the BRAF proto-oncogene, which encodes the B-Raf kinase, are associated with more aggressive, less-differentiated and therapy-resistant colorectal cancers (CRC). However, the molecular mechanisms responsible for these correlations remain unknown. Here, we report the characterization of ...
ORGANISM(S): Homo sapiens 
2014-12-21 | E-GEOD-50791 | biostudies-arrayexpress
TranscriptomicsExpression profiling of BRAFV600E melanoma cell lines upon RAF inhibition
Microarray expression analysis to identify global changes in transcription in response to RAF inhibition. Genes under RAF control were identified in a panel of BRAFV600E tumor cells, following the short-term inhibition of RAF using a pan-RAF kinase inhibitor, PLX4032 (Plexxikon). For comparison with...
ORGANISM(S): Homo sapiens 
2010-07-18 | E-GEOD-20051 | biostudies-arrayexpress
TranscriptomicsRelief of feedback inhibition of HER3 transcription by RAF and MEK inhibitors attenuates their antitumor effects in BRAF mutant thyroid carcinomas.
Analysis of transcriptome kinetics of SW1736 thyroid cancer cell line vs SK-MEL-28 melanoma cell line at various times after addition of 2 µM vemurafenib. The hypothesis tested was that SW1736 cells (vemurafenib-refractory) differentially express genes compared to SK-MEL-28 cells (vemurafenib sensit...
ORGANISM(S): Homo sapiens 
2013-02-01 | E-GEOD-37441 | biostudies-arrayexpress
ProteomicsBRAF inhibitors enhance erythropoiesis and treat anemia through paradoxical activation of MAPK
Erythropoiesis is a crucial part in hematopoietic system, while facing disruptions from various diseases conditions. Anemia and blood shortages has become global challenges, with current finite pharmacological options like EPO or glucocorticoids having limitations, especially in genetic anemias like...
ORGANISM(S): Homo Sapiens 
2024-09-09 | PXD055730 |
TranscriptomicsshRNA profiling of melanoma A375 cells stably expressing a shRNA construct for doxycycline-inducible knockdown of B-Raf (2 mg/ml Dox, antitumor efficacy of RAF inhibitor GDC-0879 involving BRAFV600E mutational status and ERK/MAPK pathway suppression
Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF ablation mediated by RNA interference relative to DMSO-treated control samples . Experiment Overall Design: We have generated melanoma A375 cells stably expressing a ...
ORGANISM(S): Homo sapiens 
2009-10-10 | E-GEOD-13487 | biostudies-arrayexpress
TranscriptomicsRNA-seq of pre-treatment and post-relapse matched samples from melanoma patients
RNA-seq of pre-treatment and post-relapse matched samples from melanoma patients RNA-seq was performed for matched samples before treatment and after relapse from six patients that have been treated with RAF or RAF+MEK inhibitors. Submitter declares that raw sequence data will be submitted to dbGaP...
ORGANISM(S): Homo sapiens 
2016-02-25 | E-GEOD-77940 | biostudies-arrayexpress
GenomicsIn vitro drug screening of RAF/MEK/ERK Inhibitors in two PDA Cell Lines
In vitro drug screening of RAF/MEK/ERK Inhibitors in two PDA Cell Lines
PRJNA1057073 | ENA
TranscriptomicsSynthetic lethal screening with small molecule inhibitors provides a pathway to rational combination therapies for melanoma
Recent data demonstrate that extracellular signals are transmitted through a network of proteins rather than hierarchical signaling pathways. This network model suggests why inhibition of a single component of a canonical pathway, even when targeting a mutationally activated driver of cancer, has in...
ORGANISM(S): Homo sapiens 
2012-12-06 | E-GEOD-39192 | biostudies-arrayexpress
UnknownRAF inhibitors promote RAS-RAF interaction by allosterically disrupting RAF autoinhibition.
Not available
S-EPMC5662619 | biostudies-literature
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