B-Raf inhibitors induce epithelial differentiation in BRAF mutant colorectal cancer cells

ABSTRACT: Mutations in the BRAF proto-oncogene, which encodes the B-Raf kinase, are associated with more aggressive, less-differentiated and therapy-resistant colorectal cancers (CRC). However, the molecular mechanisms responsible for these correlations remain unknown. Here, we report the characterization of human isogenic CRC cell line models (Caco-2, HT29, Colo-205) in which we modulate the expression of the B-RafV600E oncoprotein either by conditional cDNA or shRNA expression. Using these models in conventional and three dimensional tissue culture systems, we demonstrate that genetic depletion of endogenous B-RafV600E decreases cellular motility and invasion, while it induces hallmarks of differentiated epithelia such as the formation of functional adherens and tight junctions. Importantly, these

ORGANISM(S): Homo sapiens

SUBMITTER: Hauke Busch 

PROVIDER: E-GEOD-50791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress