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GenomicsGenome-wide localization of the RNA polymerase III transcription machinery in human cells
To characterize the pol III transcriptome in actively growing human cells, we used MR90hTeIrt lung fibroblasts, which are immortal but not transformed, with an intact Rb pathway. After formaldehyde cross-linking, chromatin was extracted and submitted to immunoprecipitation. To identify actively tr...
ORGANISM(S): Homo sapiens 
2010-02-11 | E-GEOD-18184 | biostudies-arrayexpress
TranscriptomicsThreonine-4 of the budding yeast RNAP II CTD couples transcription with Htz1-mediated chromatin remodeling
The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAP II) consists of repeated YSPTSPS heptapeptides and connects transcription with cotranscriptional events. Threonine-4 (Thr4) of the CTD repeats has been shown to function in histone mRNA 3'-end processing in chicken cells a...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-08 | E-GEOD-60181 | biostudies-arrayexpress
GenomicsGenome-wide mapping of RNA Pol III binding site (GM12878)
We report the results of chromatin immunoprecipitation following by high-thoughput sequencing (ChIP-Seq) using the GA II platform from Illumina for the human RNA Polymerase III in GM12878 cells. We have also generated a sequenced input DNA datasets for GM12878 cells. For data usage terms and condi...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-19549 | biostudies-arrayexpress
GenomicsGenome-wide mapping of RNA Pol III binding site (K562)
We report the results of chromatin immunoprecipitation following by high-thoughput tag sequencing (ChIP-Seq) using the GA II platform from Illumina for the human RNA Polymerase III in K562 cells. We have also generated a sequenced input DNA datasets for K562 cells. For data usage terms and conditi...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-19548 | biostudies-arrayexpress
GenomicsGenomic binding of Pol III transcription machinery and relationship with TFIIS distribution in mouse embryonic stem cells
RNA polymerase (RNA Pol) III synthesizes the tRNAs, the 5S ribosomal RNA and a small number of untranslated RNAs. In vitro, it also transcribes short interspersed nuclear elements (SINEs). We investigated the distribution of RNA Pol III and its associated transcription factors on the genome of mouse...
ORGANISM(S): Mus musculus 
2011-09-16 | E-MTAB-767 | biostudies-arrayexpress
GenomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20040 | biostudies-arrayexpress
GenomicsGenomic Study of RNA Polymerase II and III SNAPc-Bound Promoters Reveals a Gene Transcribed by both Enzymes and a Broad Use of Common Activators
SNAPc-dependent promoters are unique among cellular promoters in being very similar to each other, even though some of them recruit RNA polymerase II and other RNA polymerase III. We have examined all SNAPc-bound promoters present in the human genome. We find that there is a surprisingly small numbe...
ORGANISM(S): Homo sapiens 
2012-11-22 | E-GEOD-38303 | biostudies-arrayexpress
ProteomicsAnalyses of the essential C82 subunit uncover some differences in RNA polymerase III transcription between Trypanosoma brucei and Leishmania major
The 17-subunit RNA polymerase III (RNAP III) synthesizes essential untranslated RNAs such as tRNAs and 5S rRNA. In yeast and vertebrates, subunit C82 forms a stable subcomplex with C34 and C31 that is necessary for promoter-specific transcription initiation. Little is known about RNAP III transcript...
ORGANISM(S): Trypanosoma brucei Leishmania major strain Friedlin 
2024-11-13 | PXD051017 | Pride
C82  leishmania major  trypanosoma brucei  rnap iii transcription  trna  orbitrap lumos 
GenomicsNELF-dependent transcription activation
Jurkat T-cells were latently infected with a defective HIV provirus carrying a GFP reporer gene. The cells were superinfected with a lentiviral vector expressing shRNA to NELF-E. The impact of NELF-depletion on RNAP II distribution on cellular genes and the HIV provirus was measured by ChIP-Seq. D...
ORGANISM(S): Homo sapiens 
2014-03-26 | E-GEOD-47481 | biostudies-arrayexpress
TranscriptomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (II)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20071 | biostudies-arrayexpress
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