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MetabolomicsStress-related mitogen-activated protein kinases stimulate the accumulation of small molecules and proteins in Arabidopsis thaliana root exudates
A delicate balance in cellular signaling is required for plants to distinguish mutualist from parasitic organisms in its environment. Mitogen-activated protein kinase (MAPK) cascades are one of the signaling modules that mediate transduction of extracellular microbial signals into appropriate cellul...
2017-08-25 | MTBLS441 | MetaboLights
MetabolomicsPb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity
The commonly observed increased heavy metal tolerance of ectomycorrhized plants is usually linked with the protective role of the fungal hyphae covering colonized plant root tips. However, the molecular tolerance mechanisms in heavy metal stressed low-colonized ectormyocrrhizal plants characterized ...
2022-01-11 | MTBLS1847 | MetaboLights
MetabolomicsExometabolomic profiling of bacterial strains cultivated using Arabidopsis root extract as the sole carbon source
The ability of microorganisms to use root-derived metabolites as growth substrates is a key trait for success in the rhizospheric niche. However, few studies describe which specific metabolites are consumed, or to what degree microbial strains differ in their substrate consumption patterns. Here we ...
2018-10-16 | MTBLS555 | MetaboLights
MetabolomicsCell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells

Multicellular organisms such as plants contain various cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances our understanding of organization and function at the organismal level. Guard cells (GCs) are specialized epid...

2024-09-03 | MTBLS3997 | MetaboLights
ProteomicsSingle-cell proteomics differentiates Arabidopsis root cell types
Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-04-17 | MSV000094558 | MassIVE
ProteomicsShotgun proteomics of soybean root nodules infected by Bradyrhizobium japonicum
Global bottom-up proteomics analysis of proteins purified from soybean root nodules infected with either WT or nifH- mutant Bradyrhizobium japonicum. Nine glycoproteins containing Lewis-a N-glycans, with 3 distinct Lewis-a epitopes (Hex:5 HexNAc:4 dHex:3 Pent:1, Hex:4 HexNAc:4 dHex:2 Pent:1, and Hex...
ORGANISM(S): Glycine Max (ncbitaxon:3847) 
2022-01-31 | MSV000088754 | MassIVE
ProteomicsLCM-quantitative proteomics analysis of Al-sensitive tomato root cells
Aluminum toxicity in acid soil is a major stress to plant growth. The primary target is in root-tips where the epidermal and outer cortex layers of cells in the root transition zone area are more sensitive to the toxic ions. This project used laser capture microdissection (LCM) technology to isolat...
ORGANISM(S): Bemisia sp. Unknown_Th_13_12_S.lycopersicum 
2019-11-07 | PXD010459 | Pride
Tmt proteomics Laser capture microdissection Root al-sensitive cell proteomics 
ProteomicsLarge-scale proteome characterization of the cassava root
Cassava is the most important root crop in the tropics but rapid post-harvest physiological root deterioration (PPD) is a major constraint to commercial cassava production. We used label-free quantitative proteomics to generate an extensive cassava root and PPD proteome. Over 2400 unique proteins we...
ORGANISM(S): Manihot esculenta (Cassava) (Jatropha manihot) 
2014-06-03 | PXD000587 | Pride
Cassava Proteomics Root Post-harvest deterioration 
ProteomicsAl-induced root proteomics changes in stress-acclimated tomato plants
Excess levels of Al3+ become highly harmful to plant roots as the toxic ions bind to the surface of root-tips. Plants can acquire tolerance to stress after multiple generations of exposure to stress conditions. After four consecutive generations of Al treatments (G4Al+ seeds), tomato plants appeare...
ORGANISM(S): Solanum lycopersicum 
2022-02-14 | PXD018234 | Pride
Stress-acclimated tomato plants Tmt proteomics Laser capture microdissection Aluminum toxicity Outer cellular layers of root-tips 
ProteomicsProteomics studies of outer and inner cellular layers of tomato root-tips for Al stress
Excess levels of Al3+ become highly harmful to plant roots. The epidermal and outer cortical layers of root-tips experience more severe damages than the inner tissues from Al toxicity. This project used laser capture microdissection (LCM) technology to isolate the outer and inner cell layers of cel...
ORGANISM(S): Solanum lycopersicum 
2022-02-14 | PXD021994 | Pride
Tmt proteomics Laser capture microdissection Aluminum toxicity Outer and inner cellular layers of root-tips 
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