Sort   by:  
 Page size 
TranscriptomicsscRNA-seq of human fetal duodenal enteroids grown in EGF and/or NRG1
The purpose of this study was to interrogate the role of EGF-family ligands, in particular Epidermal Growth Factor (EGF) and Neuregulin 1 (NRG1), as human intestinal stem cell niche factors. An established duodenal enteroid line derived from a 20.2 week post conception biological specimen was expose...
ORGANISM(S): Homo sapiens 
2020-12-10 | E-MTAB-9720 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of intestinal epithelial organoids derived from human fetal duodenum
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of EGF or 1 ng/ml of EREG added.
ORGANISM(S): Homo sapiens 
2022-07-17 | E-MTAB-11912 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestine tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal duodenal tissue samples from 8 individual biological specimens across 7-21 weeks of gestation. The data set is composed of over 37,000 cells (between 3,000-9,000 cells per time point) from diverse intestinal lineages. Lineages ...
ORGANISM(S): Homo sapiens 
2020-12-12 | E-MTAB-9489 | biostudies-arrayexpress
TranscriptomicsDISC-seq: deciphering cell stress heterogeneity through joint mapping of cellular damage and transcriptomic landscapes in scRNA-seq
Cell heterogeneity is a fundamental feature of biological systems, driving diverse responses to stimuli and stressors, including developmental cues, diseases, and drug treatments. While single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to characterize this diversity by profiling ...
ORGANISM(S): Homo sapiens 
2026-04-19 | E-MTAB-16932 | biostudies-arrayexpress
TranscriptomicsMLL2 facilitates long-range gene regulation through LINE1 elements [scRNA-seq]
Transcriptional regulation is tightly linked to chromatin organization, with H3K4me3 commonly marking both active and bivalent promoters. In embryonic stem cells (ESC), MLL2 is essential for H3K4me3 deposition at bivalent promoters, which has been proposed to facilitate the induction of major develo...
ORGANISM(S): Mus musculus 
2026-07-12 | E-MTAB-17228 | biostudies-arrayexpress
TranscriptomicsAnalysis of intestinal crypt, villus, and polyp cells from WT and Vil1-Grem1 with or without anti-Grem1 antibody treatment by scRNA-seq
We aimed to examine changes in cellular composition of intestinal crypts, villi, and polyps from wild-type, Grem1-overexpressing (Vil1-Grem1) and anti-Grem1 antibody-treated (30 mg/kg subcutaneously weekly from the age of 6-weeks old for 10 weeks) Vil1-Grem1 mice by scRNA-seq. The proximal small bow...
ORGANISM(S): Mus musculus 
2025-03-24 | E-MTAB-14360 | biostudies-arrayexpress
TranscriptomicsScRNA-seq of healthy human PBMC samples treated with recombinant AAV8 or controls, assessed at multiple time-points
The study investigates the interaction between rAAV and the innate immune system. We conducted single cell RNA-sequencing (scRNA-seq) of PBMCs from six healthy human donors, comparing samples taken either before or after 1, 4 or 24 hrs of incubation with rAAV8. As a control, scRNA-seq was performed ...
ORGANISM(S): Homo sapiens 
2025-07-05 | E-MTAB-14760 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal kidney tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal kidney tissue samples from 2 individual biological specimens (13.7 and 15.4 weeks gestation). The data set is composed of approximately 10,000 cells from diverse renal lineages. Lineages captured include nephron progenitors, ep...
ORGANISM(S): Homo sapiens 
2020-12-20 | E-MTAB-9083 | biostudies-arrayexpress
TranscriptomicsMassively parallel single-cell RNA-sequencing of K14E7 transgenic and wildtype C57BL/6 mice
We report scRNA-seq data captured from 9,410 cells obtained from the skin of K14E7 transgenic and wildtype C57/BL6 mice. The K14E7 mouse model harbors the HPV16 E7 oncogene driven from a Keratin 14 promoter for keratinocyte-specific expression. We used scRNA-seq to detect and measure E7 transcriptio...
ORGANISM(S): Mus musculus 
2018-07-09 | E-MTAB-6429 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in matrigel or a non-adhesive alginate hydrogel after 28 days of in vitro growth. Additionally, we used scRNA-seq to profile HIOs derived in the presence of Neureguli...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10268 | biostudies-arrayexpress
Sort   by:  
 Page size