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ProteomicsUncovering Global SUMOylation Signaling Networks in a Site-Specific Manner
SUMOylation is a reversible post-translational modification essential for genome stability. Using high-resolution mass spectrometry, we have studied global SUMOylation in mammalian cells and in a site-specific manner, identifying a total of over 4,300 SUMOylation sites in over 1,600 proteins. Moreov...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080793 | MassIVE
ProteomicsSite-Selective Protein Conjugation by a Multicomponent Ugi Reaction
The chemical bioconjugation of proteins has seen tremendous applications in the past decades, with the booming of antibody-drug conjugates and their use in oncology. While genetic engineering has permitted to produce bespoke proteins featuring key (un-)natural amino acid residues poised for site-sel...
ORGANISM(S): Homo sapiens (Human) 
2024-09-09 | PXD045387 | Pride
Biomolecule  site conjugation  chemical biology  peptide mapping 
ProteomicsConjugation site characterization of antibody–drug conjugates using electron-transfer/higher-energy collision dissociation (EThcD)
Antibody–drug conjugates (ADCs) are formed by binding of cytotoxic drugs to monoclonal antibodies (mAbs) through chemical linkers. A comprehensive evaluation of the critical quality attributes (CQAs) of ADCs is vital for drug development but remains challenging owing to ADC structural heterogeneity ...
ORGANISM(S): Homo Sapiens 
2023-02-14 | PXD040111 |
ProteomicsIdentification of conjugation site of T-DM1
Ado-trastuzumab emtansine(T-DM1) is manufactured by conjugating DM1 to lysine of Trastuzumab via a non-cleavable linker in a non-specific mode.conjugated site of T-DM1 were identified using a bottom-up strategy through LC-HR-MS/MS.
ORGANISM(S): Homo sapiens environmental sample 
2025-03-05 | PXD054136 | Pride
Conjugation site Lc-ms/ms 
ProteomicsSystem-Wide Identification of Wild-Type SUMO-2 Conjugation Sites
SUMOylation is a reversible post-translational modification regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here, we present a dataset generated through a SUM...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080714 | MassIVE
ProteomicsPATCH labeling in the BSA through red light
For the liquid chromatography-mass spectrometry (LC-MS) analysis, the 500 μM AP was used to label BSA in the reaction, followed by clicking chemical conjugation with 0.1 mM azide-PEG3-biotin under 0.5 mM CuCl(2), 2 Mm Tris (3-hydroxypropyltriazolylmethyl) amine (THPTA, Sigma-Aldrich) and 5 mM ascorb...
ORGANISM(S): Bos taurus (Bovine) 
2025-07-30 | PXD057016 | Pride
Pcn bsa 
UnknownSite-selective protein conjugation at histidine.
Not available
S-EPMC6354831 | biostudies-literature
ProteomicsOrthogonal Conjugation via Endoglycosidase and Microbial Transglutaminase Enables Efficient Synthesis of Dual-Payload Antibody-Drug Conjugates
Antibody drug conjugates (ADCs) are a rapidly growing field of therapeutics which combines the highly specific targeting ability of an antibody with a cytotoxic drug to deliver the payload in a targeted manner to cancer cells. Traditional ADC construction used stochastic conjugation methods resultin...
ORGANISM(S): Homo sapiens (Human) 
2026-05-29 | PXD051902 | Pride
UnknownSite-Selective Cysteine-Cyclooctyne Conjugation.
Not available
S-EPMC6150453 | biostudies-literature
UnknownSite-Specific Antibody Conjugation to Engineered Double Cysteine Residues.
Not available
S-EPMC8308878 | biostudies-literature
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