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TranscriptomicsEffects of SPOP on gene expression in prostate cancer
SPOP is a ubiquitin ligase adaptor frequently mutated in prostate cancer. It is involved in ubiquitination and degradation of substrate proteins. We examined the impact of wild-type and mutant SPOP on the transcriptional profile of prostate cancer cells. We cloned several naturally occurring (in hum...
ORGANISM(S): Homo sapiens 
2014-10-10 | E-GEOD-59432 | biostudies-arrayexpress
GenomicsIdentification of ZMYND11 binding sites in VCaP cells with stable overexpression of either mutant (Y87C) or wild-type SPOP.
SPOP is known to bind to serine/theronine-rich degrons on substrate proteins. We identified two degron sequences within the PWWP and MYND domain of ZMYND11, a novel SPOP substrate, and investigated whether ZMYND11 may contribute to the transcriptional output of mutant SPOP in VCaP cancer cells. We m...
ORGANISM(S): Homo sapiens 
2020-12-05 | E-MTAB-7174 | biostudies-arrayexpress
ProteomicsSPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability.
Mutations in SPOP, the gene most frequently point-mutated in primary prostate cancer, are associated with a high degree of genomic instability and deficiency in homologous recombination repair of DNA but the underlying mechanisms. SPOP knockdown leads to spontaneous replication stress and impaired r...
ORGANISM(S): Homo sapiens (Human) 
2018-08-21 | PXD010527 | Pride
Prostate cancer  spop  lc-ms/ms  interactome 
TranscriptomicsStable overexpression of either mutant- or wild type SPOP in presence/absence of ΔERG in LNCaP prostate cancer cells
Purpose: Little is known about the interplay of driver mutations that never co-occur within the same cancer cells. The latter scenario has been identified in prostate cancer where recurrent gene fusions involving the oncogenic ERG transcription factor and point mutations in the ubiquitin ligase adap...
ORGANISM(S): Homo sapiens 
2020-12-10 | E-MTAB-7170 | biostudies-arrayexpress
ProteomicsSPOP mutation - SPOP Mutation Drives Prostate Tumorigenesis In Vivo through Coordinate Regulation of PI3K/mTOR and AR Signaling
Recurrent point mutations in SPOP define a distinct molecular subclass of prostate cancer. Here, we describe the first mouse model showing that mutant SPOP drives prostate tumorigenesis in vivo. Conditional expression of mutant SPOP in the prostate dramatically altered phenotypes in the setting of P...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-20 | PXD005309 | Pride
Cancer Spop Prostate Mutation 
TranscriptomicsStable over expression of the SPOP-substrate ZMYND11 in VCaP cells
SPOP is known to bind to serine/theronine-rich degrons on substrate proteins. We identified two degron sequences within the PWWP and MYND domain of ZMYND11, a novel SPOP substrate, and investigated whether ZMYND11 may contribute to the transcriptional output of mutant SPOP in VCaP cancer cells. Henc...
ORGANISM(S): Homo sapiens 
2020-12-16 | E-MTAB-7173 | biostudies-arrayexpress
ProteomicsE3 ligase substrate adaptor SPOP fine tunes the UPR of pancreatic beta cells
The Cullin 3 E3 ligase adaptor protein, SPOP, targets proteins for ubiquitination and proteasomal degradation. We previously established the β cell transcription factor (TF) and human diabetes gene PDX1 as an SPOP substrate, suggesting a functional role for SPOP in the β cell. Here, we generated a β...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-12 | PXD052527 | Pride
E3 ligase Pdx1 Spop Proteasomal degradation Diabetes 
TranscriptomicsStable overexpression and knockdown of mutant- or wild type SPOP in VCaP prostate cancer cells
Purpose: Little is known about the interplay of driver mutations that never co-occur within the same cancer cells. The latter scenario has been identified in prostate cancer where recurrent gene fusions involving the oncogenic ERG transcription factor and point mutations in the ubiquitin ligase adap...
ORGANISM(S): Homo sapiens 
2020-12-05 | E-MTAB-7165 | biostudies-arrayexpress
ProteomicsFunctional dissection of SPOP in the amino acid level reveals comprehensive functional landscape of variants on tumorigenesis
Numerous proteins display pleiotropic functions in different clinical contexts. However, molecular mechanism underlying such effects is rarely understood. The Speckle-type POZ protein (SPOP) is a typical example, showing tumor-suppressing or promoting effects due to different amino acid changes in d...
ORGANISM(S): Staphylococcus aureus 
2026-04-20 | PXD067557 | Pride
Yeast Tmt Spop 
GenomicsEffects of SPOP mutation in DU145 cells
Effects of SPOP mutation in DU145 cells
PRJNA613022 | ENA
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