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MetabolomicsStable isotope labelling of Toxoplasma gondii under iron and copper restriction

Toxoplasma uses metal nutrients like iron and copper for vital functions in their energy metabolism. Here, we have used stable isotope labelling to track the incorporation of glucose and glutamine into metabolic pathways, primarily focusing on central carbon metabolism, when these metals are disr...

2026-03-18 | MTBLS13492 | MetaboLights
MetabolomicsgeoRge: A Computational Tool To Detect the Presence of Stable Isotope Labeling in LC/MS-Based Untargeted Metabolomics
Studying the flow of chemical moieties through the complex set of metabolic reactions that happen in the cell is essential to understanding the alterations in homeostasis that occur in disease. Recently, LC/MS-based untargeted metabolomics and isotopically labeled metabolites have been used to facil...
2015-09-15 | MTBLS213 | MetaboLights
MetabolomicsGlobal Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila

System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Yet, de...

2022-06-16 | MTBLS3322 | MetaboLights
ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-08-15 | MSV000080043 | MassIVE
MetabolomicsStable Isotope–Assisted Plant Metabolomics: Combination of Global and Tracer-Based Labeling for Enhanced Untargeted Profiling and Compound Annotation
Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-b...
2020-02-09 | MTBLS1217 | MetaboLights
Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
ProteomicsStable isotope dynamic labelling of secretomes C4PR_LIV
We improve on currently-available resources by describing a mass spectrometry (MS)-based strategy using stable isotope dynamic labelling of secretomes (SIDLS) that discriminates between authentic secretory proteins and intracellular proteins within the secretome of cultured cells. By monitoring the ...
ORGANISM(S): Homo sapiens (Human) 
2019-10-08 | PXD007231 | Pride
Human cancer cells secretome dynamic labelling 
ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-01-30 | PXD000713 | Pride
Arabidopsis Silac 
MetabolomicsPhotorespiration is linked to DNA methylation by formate as one-carbon source.
DNA methylation is a key epigenetic modification regulating genome organization, stability, and gene expression. Stable DNA methylation critically relies on methyl groups provided through folate-mediated one-carbon (C1) metabolism, yet the origin and regulation of C1 supply remain elusive. Here we d...
2026-01-06 | MTBLS13444 | MetaboLights
ModelsNAD labelling dynamics
Model constructed to resample stable isotope labelling experiments using 13C-Glucose
2024-12-23 | MODEL2409150001 | BioModels
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