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TranscriptomicsTranscription profiling by array of human MCF-7 breast cell clones expressing ER-beta tagged with TAP-tag at the C-terminal or N-terminal
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.Cells were cultured in standard growth condit...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1053 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human MCF-7 cells with ER-beta tagged with TAP-tag at the C-term or N-term or expressing ER-alpha tagged
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha). All were grown in Dulbecco's modified Eagle's medium (DMEM). Then c...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1052 | biostudies-arrayexpress
ProteomicsPuf3 Tap Tag and Mass Spectrometry in Not4 Delta and WT Yeast Strains
Investigation of the proteins associated with Puf3, an RNA-binding protein, in Saccharomyces cerevisiae. The goal is to compare the Puf3 interactome in a wild-type strain and in a Not4-deleted strain (Δnot4), in order to assess the potential role of Not4 in modulating Puf3-associated complexes. Puf3...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-09-14 | PXD069853 | Pride
Yeast puf3 tap tag not4 deletion wt ap mass spectrometry yeast 
TranscriptomicsMicroarray analysis of TAP::ALG-1 associated RNAs isolated from synchronized 'wild-type' animals and 'mir-58' mutants
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein (C. elegans strain WS4303). We crossed the TAP::ALG-1 transgene into the mir-58(n4640) mutant background to generate the strain WS5041...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32942 | biostudies-arrayexpress
TranscriptomicsCLIP-seq of S. cerevisiae Drn1-TAP and Dbr1-TAP
Data for replicate Drn1-TAP and Dbr1-TAP CLIP-seq experiments to identify RNA-protein interactions Drn1-TAP and Dbr1-TAP CLIP-seq
ORGANISM(S): Saccharomyces cerevisiae 
2013-06-28 | E-GEOD-44959 | biostudies-arrayexpress
ProteomicsIntegrated Tandem Affinity Protein Purification Using the Polyhistidine plus Extra 4 Amino Acids (HiP4) Tag System
Interactome analysis of HBx using a novel tag system (HiP4-tag system).
ORGANISM(S): Homo Sapiens (human) 
2023-02-28 | PXD036256 | JPOST Repository
ProteomicsIntegrated Tandem Affinity Protein Purification Using the Polyhistidine plus Extra 4 Amino Acids (HiP4) Tag System２
Interactome analysis of TARDBP (TDP-43) using a novel tag system (HiP4-tag system).
ORGANISM(S): Homo Sapiens (human) 
2023-02-28 | PXD038445 | JPOST Repository
TranscriptomicsThree acyltransferases and a nitrogen responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas
Three acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG accumulation based on their patterns of expression. Each gene also shows increased mRNA abundance in other TAG-accumulating conditions (-S, -P, -Zn, -Fe). Insertional mutants, pda...
ORGANISM(S): Chlamydomonas reinhardtii 
2013-01-10 | E-GEOD-34585 | biostudies-arrayexpress
TranscriptomicsGene expression profile in a breast cancer cell line upon hormonal treatments
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-alpha tagged with TAP-tag at the C-term (C-TAP-ER-alpha). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) fo...
ORGANISM(S): Homo sapiens 
2012-06-25 | E-MTAB-1196 | biostudies-arrayexpress
TranscriptomicsAnalysis to evaluate the presence of 5’ cap in intragenic lncRNAs
RNA aliquots were digested with 5’-phosphate-dependent exonuclease (5'-exo) following pre-treatment with tobacco acid pyrophosphatase (TAP) to release the 5’ cap and render the RNA susceptible to 5’ exonuclease digestion (TAP+/5'-exo+). Two color array hybridizations were performed and TAP+/5'-exo+...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-62984 | biostudies-arrayexpress
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