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MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsExamining the effects of cigarette smoke on mouse lens through a multi OMIC approach
Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for...
2024-03-24 | MTBLS207 | MetaboLights
MetabolomicsA targeted MRM-based lipidomic dataset of Tenebrio molitor larvae fed diets with different protein levels
Tenebrio molitor is increasingly used as a sustainable source of nutrients for food and feed production, yet their lipid metabolic responses to dietary protein remain poorly characterized at the molecular species level. Here, we report a targeted UPLC–MS/MS lipidomics dataset of Tenebrio molitor lar...
2026-05-20 | MTBLS14531 | MetaboLights
MetabolomicsIntegration of magnetic resonance imaging and protein and metabolite CSF measurements to enable early diagnosis of secondary progressive multiple sclerosis

Molecular networks in neurological diseases are complex. Despite this fact, contemporary biomarkers are in most cases interpreted in isolation, leading to a significant loss of information and power. We present an analytical approach to scrutinize and combine information from biomarkers originati...

2018-08-13 | MTBLS558 | MetaboLights
MetabolomicsCharacterizing COPD phenotypes with a targeted signaling lipids metabolomics approach

A targeted metabolomics approach reveals distinct metabolic patterns associated with different GOLD stages, KNGF profiles and SYS subtypes in COPD patients, highlighting unique mechanisms and pathways underlying various COPD classification systems.

2025-07-21 | MTBLS11748 | MetaboLights
MetabolomicsCharacterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation

Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged ...

2026-08-24 | MTBLS12801 | MetaboLights
MetabolomicsWalnut protein hydrolysate alleviates neuroinflammation and memory impairment in naturally aged mice via a gut microbiota-metabolite-neuroinflammation axis
This study investigated age-associated alterations in the serum metabolome and the metabolic effects of walnut protein hydrolysate (WPH) supplementation in naturally aged mice. Male C57BL/6J mice at different ages (2-month, 10-month and 18-month) were used to characterize metabolic changes associate...
2026-09-04 | MTBLS15363 | MetaboLights
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