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ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
ProteomicsMore is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics
Metaproteomics is a powerful tool to characterize the structure of microbial communities and the physiology, metabolism and interactions of the species in these communities. Metaproteomics seeks to identify and quantify proteins from microbial communities on a large scale using gel electrophoresis o...
ORGANISM(S): Eukaryota (eucaryotes) Bacteria Archaea Viruses 
2019-02-12 | PXD008017 | Pride
Microbial community  microbiota  microbiome  mock community  method evaluation  microbial ecology  q exactive  liquid chromatography  gelc 
MetabolomicsA Cross-Platform Metabolomics Comparison Identifies Serum Metabolite Signatures of Liver Fibrosis Progression in Chronic Hepatitis C Patients
Metabolomics offers new insights into disease mechanisms that is enhanced when adopting orthogonal instrumental platforms to expand metabolome coverage, while also reducing false discoveries by independent replication. Herein, we report the first inter-method comparison when using multisegment injec...
2023-08-22 | MTBLS3111 | MetaboLights
UnknownComparative 1D Blue-Native electrophoresis analysis of Plasmodium falciparum and human proteins associated with cytoadherence.
Not available
S-EPMC6090645 | biostudies-literature
UnknownAutomatic DNA Diagnosis for 1D Gel Electrophoresis Images using Bio-image Processing Technique.
Not available
S-EPMC4682448 | biostudies-literature
ProteomicsDifferential BN analysis of cytoadherence complexes in P. falciparum
The pathogenesis of severe malaria has been associated with the ability of P. falciparum infected erythrocyte binding to host endothelium (a process known as cytoadherence) but the mechanisms underlying this have not been clearly described. To understand more about the molecular components that allo...
ORGANISM(S): Homo sapiens (Human) 
2018-08-09 | PXD009154 | Pride
Malaria Endothelium Human Plasmodium 
ProteomicsComparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD009860 | Pride
Mass spectrometry Breast cancer Breast milk 2d-page Proteomics Biomarkers 
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-29 | MSV000081772 | MassIVE
UnknownMore Is Not Always Better: Evaluation of 1D and 2D-LC-MS/MS Methods for Metaproteomics.
Not available
S-EPMC6383543 | biostudies-literature
UnknownInsight of Saffron Proteome by Gel-Electrophoresis.
Not available
S-EPMC6273178 | biostudies-literature
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