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ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
UnknownIsolation and identification of Enterococcus faecalis membrane proteins using membrane shaving, 1D SDS/PAGE, and mass spectrometry.
Not available
S-EPMC4887974 | biostudies-literature
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-29 | MSV000081772 | MassIVE
Proteomics1DE-MS profiling of rat cerebral cortex proteins of focal cerebral ischemia-reperfusion injury by combining SDS-PAGE, gel slicing, quantitative LC-MS/MS and profile reconstruction
A rat model of focal cerebral ischemia-reperfusion (I/R) injury was established by 2 hour-middle cerebral artery occlusion (MCAO) followed with 1, 7 and 14 days of reperfusion. Validation of the model was done by the measurement on cerebral blood flow, infarct volume, histological damage and neurol...
ORGANISM(S): Rattus Norvegicus 
2022-05-11 | PXD033793 |
UnknownSDS-PAGE fractionation to increase metaproteomic insight into the taxonomic and functional composition of microbial communities for biogas plant samples.
Not available
S-EPMC6999349 | biostudies-literature
ProteomicsProteomics analysis of B. bigemina
Protein from B. bigemina PR cell lysates was analysed by 1D SDS PAGE and in solution digestion. Digested peptides were analysed by on-line nanoflow liquid chromatography using the nanoACQUITY-nLC system (Waters MS technologies, Manchester, UK) coupled to an LTQ-Orbitrap Velos (ThermoFisher Scientifi...
ORGANISM(S): Babesia bigemina Babesia sp. 
2014-06-16 | PXD000629 | Pride
Label free 1d sds page B. bigemina 
UnknownSDS-PAGE-Based Quantitative Assay of Hemolymph Proteins in Honeybees: Progress and Prospects for Field Application.
Not available
S-EPMC10299212 | biostudies-literature
ProteomicsD.melanogaster - Whole organism, SC (Xing,proteomics,2014)
abundance based on Spectral counting, Interaction consistency score: 25.8, Coverage: 60
ORGANISM(S): 7227 
2014-00-00 | 1621505028 | PAXDB
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2014-07-16 | PXD001025 | Pride
Proteomics Collagens Mass spectrometry Nidogens Perlecan Laminin Collagen iv Basement membrane 
ProteomicsDog tear film proteome in-depth analysis
In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fract...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2015-11-23 | PXD003124 | Pride
Sds-page Maldi Dog Tear fluid Proteome 
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