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ProteomicsMore is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics
Metaproteomics is a powerful tool to characterize the structure of microbial communities and the physiology, metabolism and interactions of the species in these communities. Metaproteomics seeks to identify and quantify proteins from microbial communities on a large scale using gel electrophoresis o...
ORGANISM(S): Eukaryota (eucaryotes) Bacteria Archaea Viruses 
2019-02-12 | PXD008017 | Pride
Microbial community  microbiota  microbiome  mock community  method evaluation  microbial ecology  q exactive  liquid chromatography  gelc 
ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
UnknownMore Is Not Always Better: Evaluation of 1D and 2D-LC-MS/MS Methods for Metaproteomics.
Not available
S-EPMC6383543 | biostudies-literature
UnknownMolecular weight assessment of proteins in total proteome profiles using 1D-PAGE and LC/MS/MS.
Not available
S-EPMC1182394 | biostudies-literature
ProteomicsProfiling of fasting-feeding dynamics in mouse liver
Daily biological rhythms are orchestrated by a combination of the intrinsic circadian clock and external food/feeding-related signals which influence metabolism in health and disease. Understanding how and to which extent both circadian and fasting/feeding rhythms contribute to regulating daily phys...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-22 | PXD008944 | Pride
Metabolism Mouse Fasting Liver 1d-lc-msms Online 2d-lc-msms Feeding 
ProteomicsProteomics of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls
E. faecalis OG1RF cultures were infected with lytic phage VPE25 and proteomic profile of infected cells were compared to uninfected E. faecalis cells using 1D-LC-MS/MS analyses.
ORGANISM(S): Enterococcus phage VPE25 Enterococcus faecalis OG1RF 
2024-07-27 | PXD026873 | Pride
Enteroccocus faecalis Proteomics Phage vpe25 1d-lc-ms/ms 
ProteomicsPsychroflexus torquis 1D LC/MS
Proteorhodopsin has been an ongoing hot topic for the past decade. However the complete physiological role of this extremely widely distributed protein remains mysterious. In this study we aim to give an insight to the physiology of a proteorhodopsin-containing sea ice bacteria – Psychroflexus torqu...
ORGANISM(S): Psychroflexus torquis (strain ATCC 700755 / ACAM 623) 
2015-07-24 | PXD000673 | Pride
Salinity Proteorhodopsin Sea ice 1d lc/ms Psychroflexus torquis 
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-29 | MSV000081772 | MassIVE
ProteomicsIschemia/reperfusion kidney injury: 1D and 2D LC-MS differential protein expression analysis of urine from patients undergoing CBP surgery
Urine was collected from 10 patients undergoing cardiac bypass (CBP) surgery following anesthesia induction. The study population divided into 2 post-operative outcomes: 5 experiencing acute kidney injury (AKI), and 5 not. A 1D LC-MS of individual patients was performed, as well as 2D LC-MS of pools...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-05-31 | MSV000081129 | MassIVE
ProteomicsMembrane proteins of Planctopirus limnophila
Bacteria of the phylum Planctomycetes have been postulated to be the ‘missing link’ between prokaryotes and eukaryotes. Although phylogenetically the Planctomycetes were grouped into Gram-negative bacteria, it has been reported that they have no outer membrane. Here we present 1D-gel-LC/MS/MS based...
ORGANISM(S): Planctopirus limnophila DSM 3776 
2017-04-18 | PXD005738 | Pride
Planctomycetes 1d-gel-lc-ms/ms Membrane proteins 
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