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ProteomicsMembrane proteins of Planctopirus limnophila
Bacteria of the phylum Planctomycetes have been postulated to be the ‘missing link’ between prokaryotes and eukaryotes. Although phylogenetically the Planctomycetes were grouped into Gram-negative bacteria, it has been reported that they have no outer membrane. Here we present 1D-gel-LC/MS/MS based...
ORGANISM(S): Planctopirus limnophila DSM 3776 
2017-04-18 | PXD005738 | Pride
Planctomycetes 1d-gel-lc-ms/ms Membrane proteins 
ProteomicsComprehensive mapping of the bull sperm surface proteome
Nitrogen cavitation of bovine sperm to isolate plasma membrane. FASP digestion using trypsin. Data acquired using 5 workflows: (1) Unfractionated on a 5600 TripleTOF; (2) SCX-ESI-LC-MS/MS on a QSTAR Elite; (3) 1D Gel-LC-ESI-LC-MS/MS on a QSTAR Elite; (4) SCX-LC-MALDI-MS/MS on a UltrafleXtreme; (5) ...
ORGANISM(S): Bos taurus (Bovine) 
2020-01-27 | PXD000007 | Pride
Sperm Ultraflextreme Bovine 5600 tripletof Qstar elite Plasma membrane 
UnknownProteomic analyses of native brain K(V)4.2 channel complexes.
Not available
S-EPMC2826169 | biostudies-literature
UnknownMolecular weight assessment of proteins in total proteome profiles using 1D-PAGE and LC/MS/MS.
Not available
S-EPMC1182394 | biostudies-literature
ProteomicsHuman exosome proteome - Human urinary exosomes as innate immune effectors
Proteomics study of human urine exosomes. This data describes the proteomic complement of the human exosomal urine fraction from 10 healthy volunteers (5 male, 5 female) aged 23 to 36 years. 50 µg protein from each sample were fragmented on a 4%/12% SDS-polyacrylamide gel. Following staining, each g...
ORGANISM(S): Homo sapiens (Human) 
2016-08-25 | PXD000117 | Pride
Exosome Urine Human Proteome 
UnknownMultiple-approaches to the identification and quantification of cytochromes P450 in human liver tissue by mass spectrometry.
Not available
S-EPMC2939981 | biostudies-literature
ProteomicsIdentification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD001004 | Pride
Silac Lmtk3 Breast cancer Integrin b1 Grb2 
ProteomicsProteaphagy contributes to bortezomib resistance in mantle cell lymphoma
TUBE and GST enrichment followed by 1D-gel-LC-MS analysis on cell exract from bortezomib (BTZ)-resistant or sensitive mantle cell lymphoma cells.
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD011867 | Pride
Ubiquitin proteasome system Lymphoma Proteaphagy Bortezomib resistance Tubes 
OtherRapid Analyses of Proteomes and Interactomes Using an Integrated Solid-Phase Extraction-Liquid Chromatography-MS/MS System.
Data from ProteomeXchange, PXD ID: PXD001111. Experiment: NYLON_Hela, file: OR7_20130627_NB_Nylon_Hela_1ug_top20_1.mzml. Published as part of J Proteome Res. 2014 Dec 8 . From the Abstract: {{i}} Here, we explore applications of a LC system using disposable solid-phase extraction (SPE) cartridges a...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310005326 | GPMDB
Resource Reanalysis 
UnknownComparison of in-gel protein separation techniques commonly used for fractionation in mass spectrometry-based proteomic profiling.
Not available
S-EPMC4234072 | biostudies-literature
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