2-n-acetylglucosaminyltransferase 1

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Proteomics (52)
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Instrument Platform

Illumina NovaSeq 6000 (16)
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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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Chromium Controller, 10x Genomics and Chromium X, 10x Genomics (1)
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2014 (3)
2019 (3)
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2013 (2)
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2011 (1)
2008 (1)
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CHEBI ID

CHEBI:16467 (3)
CHEBI:16015 (3)
CHEBI:16857 (3)
CHEBI:17053 (3)
CHEBI:15428 (3)
CHEBI:25682 (2)
CHEBI:15729 (2)
CHEBI:16283 (2)
CHEBI:28229 (2)
CHEBI:17895 (2)
CHEBI:17295 (2)
CHEBI:16643 (2)
CHEBI:18050 (2)
CHEBI:30915 (2)
CHEBI:17196 (2)
CHEBI:6650 (2)
CHEBI:17750 (2)
CHEBI:16414 (2)
CHEBI:17203 (2)
CHEBI:17115 (2)
CHEBI:16977 (2)
CHEBI:15603 (2)
CHEBI:17191 (2)
CHEBI:30769 (2)
CHEBI:15741 (2)
CHEBI:15891 (2)
CHEBI:17929 (2)
CHEBI:16737 (2)
CHEBI:37054 (1)
CHEBI:31011 (1)

Metabolite Name

Malate (2)
Citrulline (2)
Carnitine (2)
Creatine (2)
Citrate (2)
L-Aspartate (2)
Creatinine (2)
Asparagine (2)
Betaine (2)
Sarcosine (2)
Tryptophan (2)
Tyrosine (2)
Lysine (2)
Methionine (2)
Isoleucine (2)
Leucine (2)
Valine (2)
Serine (2)
Aspartic acid (2)
Phenylalanine (2)
Glycine (2)
Arginine (2)
Alanine (2)
Threonine (2)
Taurine (2)
Succinate (2)
Proline (2)
Histidine (2)
Glutamine (2)
N(omega)-methyl-L-arginine (NMMA) (1)

First Public Date

2022 (1)
2021 (1)

Study type

RNA-seq of coding RNA (12)
RNA-seq of coding RNA from single cells (11)
Transcription profiling by array (10)
ChIP-seq (6)
DNA-seq (5)
RNA-seq of total RNA (4)
Unknown experiment type (3)
Animal - One-color microarray (3)
ATAC-seq (3)
Animal - High-throughput sequencing (1)
Transcription profiling by tiling array (1)
ScATAC-seq (1)
Single nucleus RNA sequencing (1)
Methylation profiling by high throughput sequencing (1)

Release Date

2025 (22)
2026 (18)
2012 (8)
2005 (7)
2021 (4)
2017 (4)
2016 (3)
2015 (3)
2013 (3)
2011 (3)
2010 (3)
2024 (3)
2023 (2)
2006 (2)
2014 (2)
2007 (2)
1999 (2)
2008 (1)
2022 (1)
2020 (1)
2019 (1)
2000 (1)

Lab affiliation

Centre for Organismal Studies (COS) Heidelberg Im Neuenheimer Feld 360 69120 Heidelberg Fon +49 6221 54-6286 Fax +49 6221 54-5859 (1)

Tags

xref:PubMed:34864058 (1)

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Inactivation of N-acetylglucosaminyltransferase I and alpha1,3-fucosyltransferase genes in Nicotiana tabacum BY-2 cells results in glycoproteins with highly homogeneous, high-mannose N-glycans - Herman and coworkers Frontiers in Plants Sciences

This work was performed under the collaboration of the LIBST group from the University of Louvain-la-Neuve UClouvain (https://uclouvain.be/en/research-institutes/libst) and the MSLab group from the University of Liege ULiege (http://www.mslab.ulg.ac.be/team/). Identification of the N-glycans librar...

ORGANISM(S): Nicotiana Tabacum Bright Yellow-2

2020-12-28 | MSV000086640 | MassIVE

Crystal structures of β-1,4-N-acetylglucosaminyltransferase 2: structural basis for inherited muscular dystrophies.

Not available

S-EPMC8025878 | biostudies-literature

Golgi phosphoprotein 3 determines cell binding properties under dynamic flow by controlling Golgi localization of core 2 N-acetylglucosaminyltransferase 1.

Not available

S-EPMC3501027 | biostudies-literature

Pancreatic cancer FG cells: Core 3 synthase stable expression vs vector control

Purpose: Core 3 derived glycans, a major type of O-glycan expressed by normal epithelial cells of the gastrointestinal tract, are downregulated during malignancy, because of loss of expression of functional β3-N-acetylglucosaminyltransferase-6 (core 3 synthase). We investigated the expression of cor...

ORGANISM(S): Homo sapiens

2012-11-21 | E-GEOD-42417 | biostudies-arrayexpress

MGAT3 and MGAT5 overexpression alters the protein cargo of extracellular vesicles released by metastatic melanoma cells

Extracellular vesicles (EVs) are potential non-invasive diagnostic, prognostic, and therapeutic tools. Additionally, they are important contributors to tumorigenesis. Glycosylation has been found to modulate the composition of the EV proteome, and increased amounts of beta 1,6-branched N-glycans, sy...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

2024-11-08 | MSV000096340 | MassIVE

Transcriptional profiling of Entamoeba histolytica trophozoites

This SuperSeries is composed of the following subset Series: GSE2978: Entamoeba heat shock experiments GSE2979: Entamoeba plus Caco2 cells Abstract: We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS)...

ORGANISM(S): Entamoeba histolytica

2006-03-07 | E-GEOD-4400 | biostudies-arrayexpress

ScRNA-Sequencing of Postnatal day 12 (P12) tetstes in Ogt conditional knock out and Wild-Type mice

Utilizing single-cell RNA sequencing, analyzed the impact of OGT deficiency on RNA metabolism across distinct germ cell populations and assessed the influence of O-GlcNAcylation loss on developmental trajectories within spermatogenic lineages.

ORGANISM(S): Mus musculus

2026-08-01 | E-MTAB-15514 | biostudies-arrayexpress

The directed migration of gonadal distal tip cells in Caenorhabditis elegans requires NGAT-1, a ß1,4-N-acetylgalactosaminyltransferase enzyme.

Not available

S-EPMC5560668 | biostudies-literature

Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.

Not available

S-EPMC6360353 | biostudies-literature

Core 3 synthase is down-regulated in colon carcinoma and profoundly suppresses the metastatic potential of carcinoma cells.

Not available

S-EPMC555466 | biostudies-literature