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GenomicsGenome-wide mapping of 5-hydroxymethylcytosine (5hmC) during differentiation of P19.6 and 3T3-L1 cells
Enhancers are developmentally-controlled transcriptional regulatory regions whose activities are modulated through histone modifications or histone variant deposition. Here, we show by genome-wide mapping that the newly discovered DNA modification 5-hydroxymethylcytosine (5hmC) is dynamically associ...
ORGANISM(S): Mus musculus 
2012-06-04 | E-GEOD-38407 | biostudies-arrayexpress
TranscriptomicsDetection of microRNA expression during 3T3-L1 pre-adipocyte differentiation using a custom made microarray chip.
Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether micro (mi)RNAs play a...
ORGANISM(S): Mus musculus 
2010-05-18 | E-GEOD-16229 | biostudies-arrayexpress
GenomicsPropagation of Adipogenic Signals through an Epigenomic Transition State
The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), p300, mediator subunit 1, and h...
ORGANISM(S): Mus musculus 
2010-05-20 | E-GEOD-21898 | biostudies-arrayexpress
TranscriptomicsRNA-seq of ZFP30 knock-down and knock-out in mouse 3T3-L1 and IBA cells during adipogenic differentiation
We show that a hitherto poorly characterized KRAB domain-containing zinc-finger (ZF) transcription factor, ZFP30, positively regulates adipogenesis. We demonstrate ZFP30’s function in murine in vitro and in vivo models, as well as in human stromal vascular fraction cells. We reveal through mechanist...
ORGANISM(S): Mus musculus 
2019-03-01 | E-MTAB-6995 | biostudies-arrayexpress
TranscriptomicsEffects of supernatants of USP2 knockdown HL-60 cells and mock transfected HL60 cells on differentiated 3T3-L1 cells
Ubiquitin specifc protease (USP) 2 modulates various cellular responces including carcinogenesis, spermatogenesis, and glucose metabolism. So far, we established USP2 knockdown HL-60 cells to explore roles of USP2 in macrophages. In this experiment, we investigated whether USP2 knockdown HL-60 cells...
ORGANISM(S): Mus musculus 
2013-08-27 | E-GEOD-43313 | biostudies-arrayexpress
TranscriptomicsTranslational control in early adipogenesis
In obesity, adipose tissue expands by increasing the volume of existing adipocytes (hypertrophy), increasing the number of small adipocytes (hyperplasia) or both. Much is known about transcriptional control during adipogenesis, including early events. However, translational control plays a pivotal r...
ORGANISM(S): Mus musculus 
2012-03-28 | E-GEOD-29744 | biostudies-arrayexpress
GenomicsEpigenomic profiling of 3T3-L1 adipogenesis
3T3-L1 pre-adipocyte cells were grown to confluence and induced to differentiate in adipogenic media. Examination of 6 histone modifications and CTCF at 4 time points and PPARG at 1 time point using ChIP-Seq
ORGANISM(S): Mus musculus 
2010-09-30 | E-GEOD-21365 | biostudies-arrayexpress
TranscriptomicsExpression profiling of 3T3-L1 adipogenesis
3T3-L1 pre-adipocyte cells were grown to confluence and induced to differentiate in adipogeneic media. Two technical replicates from four time points relative to induction of adipogenesis (day 0)
ORGANISM(S): Mus musculus 
2010-09-30 | E-GEOD-20696 | biostudies-arrayexpress
TranscriptomicsLSD1 knockdown in 3T3-L1 preadipocytes
Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and atherosclerotic complications. This is usually coupled with increased macrophage infiltration in the adipose tissue and a defect in adipocyte differentiation that re...
ORGANISM(S): Mus musculus 
2013-12-31 | E-GEOD-25423 | biostudies-arrayexpress
TranscriptomicsMolecular architecture of transcription factor hotspots in early adipogenesis
Here, we have used digital genomic footprinting to precisely define protein localization for several adipogenic transcription factors at a genome-wide level. In combination with ChIP-seq data, these analyses reveal novel molecular insight into the organization of transcription factors at hotpot regi...
ORGANISM(S): Mus musculus 
2014-05-08 | E-GEOD-57415 | biostudies-arrayexpress
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