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ProteomicsTargeted RNase H-mediated Extraction of crosslinked RBPs (TREX) for deciphering the region-specific interactome of 45S rRNA
We used Targeted RNase H-mediated Extraction of crosslinked RBPs (TREX)to assess the endogenous region-specific binding partners of 45S rRNA in human HCT116 cells. We performed TREX experiments against the full-length 45S, as well as each individual region (5'ETS. 18S, ITS1, 5.8S, ITS2, 28S, and 3'E...
ORGANISM(S): Homo sapiens (Human) 
2023-12-27 | PXD044659 | Pride
45s rrna  pre-rrna  rrna  5'ets  its1  its2  3'ets  18s  28s  5.8s  trex  rbp  rna interactome  lc-ms/ms  quantitative proteomics 
TranscriptomicsExpression of sense and antisense transcripts at the ribosomal locus
Epigenetic methyl-CpG silencing of the ribosomal RNA (rRNA) genes is thought to down-regulate rRNA synthesis in mammals. In contrast, we now show that CpG methylation in fact positively influences rRNA synthesis and processing. Human HCT116 cells, inactivated for DNMT1 and DNMT3b or treated with aza...
ORGANISM(S): Homo sapiens 
2010-05-16 | E-GEOD-17042 | biostudies-arrayexpress
UnknownPre-45s rRNA promotes colon cancer and is associated with poor survival of CRC patients.
Not available
S-EPMC5671943 | biostudies-literature
UnknownSingle Copies of the 5S rRNA Inserted into 45S rDNA Intergenic Spacers in the Genomes of Nototheniidae (Perciformes, Actinopterygii).
Not available
S-EPMC10138776 | biostudies-literature
UnknownSirtuin 7 promotes 45S pre-rRNA cleavage at site 2 and determines the processing pathway.
Not available
S-EPMC6771141 | biostudies-literature
TranscriptomicsGlobal Run-On sequencing (GRO-seq) of HeLa cells under CDK9 inhibitor tratment to study elongation checkpoints at both ends of RNA polymerase II-transcribed genes.
Global Run-On sequencing (GRO-seq) was carried out on nuclei isolated from HeLa cells after 30 minutes of treatment with CDK9 inhibitors KM05382 and DRB and from two untreated controls. Libraries of nascent RNAs were generated and sequenced using Illumina Hi-seq technology. GRO-seq reads were proces...
ORGANISM(S): Homo sapiens 
2015-02-26 | E-MTAB-3360 | biostudies-arrayexpress
UnknownUnstable Inheritance of 45S rRNA Genes in <i>Arabidopsis thaliana</i>.
Not available
S-EPMC5386868 | biostudies-literature
UnknownExpression of rice 45S rRNA promotes cell proliferation, leading to enhancement of growth in transgenic tobacco.
Not available
S-EPMC6543702 | biostudies-literature
Unknown45S rDNA Diversity In Natura as One Step towards Ribosomal Heterogeneity in Arabidopsis thaliana.
Not available
S-EPMC10386311 | biostudies-literature
UnknownOrganization and expression analysis of 5S and 45S ribosomal DNA clusters in autotetraploid Carassius auratus.
Not available
S-EPMC8569995 | biostudies-literature
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