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Purpose: The plant hormone abscisic acid (ABA) coordinates responses leading to desiccation tolerance in all land plants. RNA-seq was employed to obtain differential expression data in response to ABA, dehydration and mannitol treatments in wild-type tissue. This was compared against the targeted ge...
ORGANISM(S): Physcomitrella patens 
We found that TOR kinase regulates the plant environmental stress responses through phosphorylation at a conserved serine residue of Abscisic acid receptor PYR1/PYLs/RCARs. The phosphorylation inhibits the function of PYLs and prevents the binding of PYLs to abscisic acid and its downstream substrat...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-12-01 | PXD003746 | Pride
We used RNA-seq platform to determine role of a splicing factor RBM25 in regulation of gene expression and pre-mRNA splicing. We found that a loss-of-function allele of RBM25, rbm25-1, causes up- and down-regulation of a large number of genes. We further found that the rbm25-1 mutation results in d...
ORGANISM(S): Arabidopsis thaliana 
Wheat seed germination and seminal root growth can be inhibited by treatment with exogenous ABA We used Affymetrix GeneChip Wheat Genome Array to detail transcriptional programs affected by ABA during imbibition After-ripened seeds imbibed in ABA for 24 h were used for RNA extraction and hybridizati...
ORGANISM(S): Triticum aestivum 
We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome under drought, cold, high-salinity and ABA treatment conditions and idenfied many stress- or ABA- responsive putative functional RNAs and fully-overlapping sense-antisense transcripts in Arabidopsis genome. Keywords: st...
ORGANISM(S): Arabidopsis thaliana 
Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with or without 50 µM ABA for 6 h.
ORGANISM(S): Arabidopsis thaliana 
To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. total samples...
ORGANISM(S): Arabidopsis thaliana 
Plant hormones involved in environmental stresses, namely abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), have been shown to interact with each other in a complex manner. To address the network of the hormone interactions, we have investigated the changes in expression under multip...
ORGANISM(S): Arabidopsis thaliana 
4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatm...
ORGANISM(S): Populus tremula x Populus alba 
Early rapid changes in response to the phytohormone abscisic acid (ABA) have been observed at the transcript level, but little is known how these transcript changes translate to changes in protein abundance under the same conditions. Here we have performed a global quantitative analysis of transcrip...
ORGANISM(S): Arabidopsis thaliana 
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