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TranscriptomicsAbsolute quantification of miRNAs
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-15835 | biostudies-arrayexpress
TranscriptomicsAbsolute quantification of protein production reveals principles underlying protein synthesis rates
A quantitative view of cellular functions requires precise measures of the rates of biomolecule production, especially proteins-the direct effectors of biological processes. Here we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resul...
ORGANISM(S): Escherichia coli 
2014-04-24 | E-GEOD-53767 | biostudies-arrayexpress
TranscriptomicsAbsolute quantification of CD34(+)/CD133(-) hematopoietic stem cells
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): synthetic construct 
2009-10-26 | E-GEOD-15831 | biostudies-arrayexpress
TranscriptomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
Recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells. In contrast, single cell proteomics analyses have been restricted to targeted analyses, for example using flow cytometry with GFP fusions or mass cytometry. Here, we performed glob...
ORGANISM(S): Xenopus laevis 
2014-08-04 | E-GEOD-56586 | biostudies-arrayexpress
TranscriptomicsRelative quantification of miRNAs across multiple experiments
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): synthetic construct 
2010-06-20 | E-GEOD-15833 | biostudies-arrayexpress
GenomicsAbsolute quantification of mammalian microRNAs
Absolute quantification of mammalian microRNAs
PRJNA1140118 | ENA
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
ProteomicsAriadne’s Thread in the Analytical Labyrinth of Membrane Proteins – Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins
The field of systems biology has been rapidly increasing in the past decade. However, the data produced by “omics” approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the present study, a novel approach for large-scale...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2019-08-26 | PXD014272 | Pride
Shotgun-ms Absolute quantification Targeted-ms Snap-tag Bacillus subtilis Membrane proteins 
ProteomicsAbsolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics ana...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-01 | PXD015214 | Pride
Absolute proteome quantification/ histone modification quantification Prc2 H3k27 methylation Drosophila 
ProteomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of single Xenopus laevis eggs using mass spec...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2014-07-23 | PXD000902 | Pride
Global absolute quantification reveals tight regulation of protein expression in single xenopus eggs 
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