Cultured epidermal keratinocyte controls used for IFNg, TNFa and IL1 treatment. Interferon (IFN)-gamma, is a multifunctional, immunomodulatory cytokine with cell type-specific antiviral activities, particularly important in skin, where it is implicated in many diseases ranging from warts to psoriasi...
Myc-tagged PrimPol expressed in HEK-293T cells was immunoprecipitated and separated by SDS-PAGE. Protein bands were excised and subjected to in-gel tryptic digestion and phosphopeptide enrichment by Fe-IMAC.