Dataset Information
PrimPol-phosphoproteomics by IP-GeLC-MS/MS
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Bunsyo Shiotani
PROVIDER: PXD043419 | JPOST Repository | Tue Aug 22 00:00:00 BST 2023
REPOSITORIES: jPOST
Publications
An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress.
Nature communications 20230817 1
Activation of the KRAS oncogene is a source of replication stress, but how this stress is generated and how it is tolerated by cancer cells remain poorly understood. Here we show that induction of KRAS<sup>G12V</sup> expression in untransformed cells triggers H3K27me3 and HP1-associated chromatin compaction in an RNA transcription dependent manner, resulting in replication fork slowing and cell death. Furthermore, elevated ATR expression is necessary and sufficient for tolerance of KRAS<sup>G12V ...[more]