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ProteomicsChemoproteomic Profiling by Cysteine Fluoroalkylation Reveals the DNA Repair Protein XRCC5 as a Functional Target of Myrocin G
Chemoproteomic profiling of cysteines has emerged as a powerful method for screening the proteome-wide targets of cysteine-reactive frag-ments, drugs and natural products. Herein, we report the development and an in-depth evaluation of a tetrafluoroalkyl benziodoxole as a cyste-ine-selective chemopr...
ORGANISM(S): Homo sapiens (Human) 
2021-12-13 | PXD029255 | Pride
Human Q exactive hf-x Natural product Hypervalent iodine Target id Myrocin 
ProteomicsNatural product target identification of wheldone as a KIF11 inhibitor in ovarian cancer using the DiffPOP (Differential Protein Precipitation) method
Wheldone, a fungal metabolite, was identified as a cytotoxic compound in high-grade serous ovarian cancer (HGSOC). Wheldone triggered caspase 3/7–dependent apoptosis and reduced migration, invasion, and spheroid growth. Wheldone was also capable of triggering apoptosis in chemoresistant HGSOC models...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070302 | Pride
Ovarian cancer Cancer biology Proteomics Target identification Natural products 
ProteomicsEthynylation of Cysteines from Peptides to Proteins in Living Cells
Efficient methods to introduce bioorthogonal groups, such as terminal alkynes, into biomolecules are important tools for chemical biology. State-of-the-art approaches are based on the introduction of a linker between the targeted amino acid and the alkyne, and still present limitations of either rea...
ORGANISM(S): Homo sapiens (Human) 
2020-04-07 | PXD018165 | Pride
Human Qexactive hf-x Cysteine ethynylation Hela 
ProteomicsMixed Alkyl/Aryl Phosphonates Identify Metabolic Serine Hydrolases as Antimalarial Targets
Malaria, caused by Plasmodium falciparum, remains a significant health burden. The barrier for developing anti-malarial drugs is the ability of the parasite to rapidly generate resistance. We previously demonstrated that Salinipostin A (SalA), a natural product, potently kills parasites by inhibitin...
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum (isolate 3D7) 
2024-07-15 | PXD051499 | Pride
Plasmodium Lipid metabolism Serine hydrolases Activity-based probes 
Proteomics1-deoxydihydroceramide causes anoxic death by impairing chaperonin-mediated protein folding
Ischemic heart disease and stroke are the most common causes of death world-wide. In both pathologies anoxia, the lack of oxygen, triggers profound metabolic and cellular changes. Sphingolipids have been implicated in anoxia injury, but the pathomechanism is unknown. Here we show that injury is caus...
ORGANISM(S): Caenorhabditis elegans 
2019-08-20 | PXD014573 | Pride
C. elegans Qexactive plus Lc-ms/ms 
ProteomicsUnveiling Cellular Communications through Rapid Pan-Membrane-Protein Labeling
The dynamic protein distribution within and across the plasma membrane is pivotal in regulating cell communications. Yet, methods facilitating rapid labeling, applicable across diverse cell types, and amenable to multiplexed live imaging are scarce. We discovered that live mammalian cell surface pro...
ORGANISM(S): Mus musculus (Mouse) 
2025-02-28 | PXD061317 | Pride
Mouse Nhs-biotin Plasma membrane 
ProteomicsSolid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows
Macrocyclic peptides are attractive for chemoproteomic applications due to their modular synthesis and potential for high target selectivity. We describe a solid phase synthesis method for the efficient generation of libraries of small macrocycles that contain an electrophile and alkyne handle. The ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-02 | PXD039931 | Pride
Sufex Chemoproteomics Electrophile Macrocycles Fluorosulfate 
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