Sort   by:  
 Page size 
MetabolomicsThe Poly (ADP-Ribose) Polymerase inhibitor Veliparib and Radiation Cause Significant Cell Line Dependent Metabolic Changes in Breast Cancer Cells
Breast tumors are characterized into different subtypes based on their surface marker expression, which affects their prognosis and treatment. For example, triple negative breast cancer cells (ER-/PR-/Her2-) show reduced susceptibility towards radiotherapy and chemotherapeutic agents. Poly (ADP-ribo...
2016-11-07 | MTBLS337 | MetaboLights
MetabolomicsMetabolic responses induced by DNA damage and poly (ADP-ribose) polymerase (PARP) inhibition in MCF-7 cells
Genomic instability is one of the hallmarks of cancer. Several chemotherapeutic drugs and radiotherapy induce DNA damage to prevent cancer cell replication. Cells in turn activate different DNA damage response (DDR) pathways to either repair the damage or induce cell death. These DDR pathways also e...
2015-08-01 | MTBLS177 | MetaboLights
ProteomicsCellular ADP-ribosylome characterization with HCD and EThcD
Protein ADP-ribosylation is a post-translational modification involved in important physiological and pathological processes. Here, we present an optimized method using the Orbitrap Fusion Tribrid mass spectrometer, which drastically improves the overall ADP-ribosylation site localization score and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-28 | MSV000080008 | MassIVE
ProteomicsEngineering Af1521 macro domain increases ADP-ribose binding affinity and leads to the identification of more ADP-ribosylated proteins
Protein ADP-ribosylation is a covalent, reversible post-translational modification that has important functions in several cellular mechanisms. The identification of modified proteins in cells has proven challenging and, due to the low abundance of protein ADP-ribosylation, has mainly been possible ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-20 | PXD016686 | Pride
Ap-ms Af1521 macro domain Adp-ribosylation Lc-ms/ms 
TranscriptomicsRNAi of human MCF7 cells knocked down for PARP-1 or PARGs
Poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG) are enzymes that modify target proteins in the nucleus by the addition and removal, respectively, of ADP-ribose polymers. Although a role for PARP-1 in gene regulation has been well established, the role of PARG is le...
ORGANISM(S): Homo sapiens 
2009-10-30 | E-GEOD-12952 | biostudies-arrayexpress
ProteomicsComprehensive ADP-ribosylome analysis identifies tyrosine as an ADP-ribose acceptor site
Despite recent mass spectrometry (MS)-based breakthroughs, comprehensive ADP-ribose (ADPr)-site identification and localization remain challenging. Here, we report the establishment of an unbiased, multistep ADP-ribosylome data analysis workflow that led to the identification of tyrosine as a novel ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-28 | PXD009948 | Pride
Tyrosine adp-ribosylation Parp1 Artd1 Hpf1 Rsp3a Genotoxic stress Adp-ribosylation 
ProteomicsPARG is Essential for Pol-theta-Mediated DNA End-Joining by Removing Repressive Poly-ADP Ribose Marks
DNA polymerase theta (Pol-theta)-mediated end-joining (TMEJ) repairs DNA double-strand breaks and confers resistance to genotoxic agents. How Pol-theta is regulated at the molecular level to exert TMEJ remains unknown. We find that Pol-theta interacts with and is PARylated by PARP1 in vitro and in c...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000092839 | MassIVE
TranscriptomicsPaternal poly(ADP-ribose) metabolism modulates retention of inheritable sperm histones and early embryonic gene expression [PJ34_sperm_MNaseb]
To achieve the extreme nuclear condensation necessary for sperm function, most histones are replaced with protamines during spermiogenesis in mammals. Mature sperm retain only a small fraction of nucleosomes, which are, in part, enriched on gene regulatory sequences, and recent findings suggest tha...
ORGANISM(S): Mus musculus 
2014-08-30 | E-GEOD-56282 | biostudies-arrayexpress
ProteomicsGas-phase Fragmentation of ADP-Ribosylated Peptides: Arginine-Specific Side-Chain Losses and their Implication in Database Searches
ADP-ribosylation is a reversible post-translational modification of proteins that has been linked to many biological processes. The identification of ADP-ribosylated proteins and of their acceptor amino acids remains a significant challenge. The attachment sites of the modification are difficult to ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-05-19 | PXD017013 | Pride
Post-translational modification Arginine Mass spectrometry Marker ion Neutral loss Adp-ribosylation Orbitrap fusion 
TranscriptomicsMechanistic Rationale for Inhibition of Poly(ADP-Ribose) Polymerase in ETS Gene Fusion Positive Prostate Cancer
To identify commonly altered genes between ERG, PARP1, and DNA-PKcs. Two-condition experiment: Each siRNA, targeting either DNA-PKcs or PARP1, was compared to a control RNA.
ORGANISM(S): Homo sapiens 
2011-05-18 | E-GEOD-27723 | biostudies-arrayexpress
Sort   by:  
 Page size