Proteomics

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Engineering Af1521 macro domain increases ADP-ribose binding affinity and leads to the identification of more ADP-ribosylated proteins


ABSTRACT: Protein ADP-ribosylation is a covalent, reversible post-translational modification that has important functions in several cellular mechanisms. The identification of modified proteins in cells has proven challenging and, due to the low abundance of protein ADP-ribosylation, has mainly been possible via enrichment methodologies using ADP-ribose binding domains. Here, using random mutagenesis and in vitro selection with an ADP-ribosylated histone peptide, we engineered the archaeal Af1521 macro domain to generate an engineered isoform containing nine mutations that displays significantly increased affinity towards ADP-ribose. Comparison of the wild-type and the engineered Af1521 macro domains using our proteomic ADP-ribosylome enrichment workflow demonstrated an increased identification rate of ADP-ribosylated proteins with the engineered Af1521.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacillus thuringiensis   Aspergillus nidulans FGSC A4   Taeniopygia guttata   Allexis   Planococcus halocryophilus Or1   Lactobacillus johnsonii N6.2   Acaryochloris marina MBIC11017   Saccharomyces cerevisiae S288C   Pyricularia oryzae   anaerobic digester metagenome   Bacillus altitudinis   Lachancea waltii NCYC 2644   Coccinia grandis   Coffea canephora   Crithidia fasciculata   Desulfofundulus kuznetsovii DSM 6115   Coffea arabica   Tannerella forsythia 92A2   Dehalobacter restrictus   Phytophthora capsici LT1534   Neisseria meningitidis MC58   Rhizobium sp. Leaf68   Oryctolagus sp. 'Rabbit_OD'   Roseburia   Moneuplotes crassus   Populus tremula   Stegodyphus mimosarum   Rickettsia conorii str. Malish 7   environmental samples   Pseudomonas fluorescens SBW25   Hebomoia glaucippe   Macaca nemestrina   Nicotiana sylvestris   Fusarium fujikuroi IMI 58289   Deinococcus radiodurans   Coprococcus eutactus   Pandanus amaryllifolius   Geobacillus kaustophilus   Bordetella pertussis Tohama I   Vitis vinifera   Fraxinus mandshurica   Yersinia pestis   Buchnera aphidicola (Acyrthosiphon pisum)   Sulfurihydrogenibium   Paulownia fortunei   soil bacterium 10G-05   Homo sapiens   Capsicum annuum var. annuum   Nilaparvata lugens   Thermochaetoides thermophila DSM 1495   Methylocystis sp. SC2   Cervus elaphus elaphus   Clostridium phage c-st   Streptococcus pneumoniae R6   Mollivirus sibericum   Eucalyptus   Serpentes   Salmo salar   Onchocerca lupi   Trichoderma harzianum CBS 226.95   Thermococcus gammatolerans   Pedobacter sp.   Acetivibrio thermocellus   Francisella tularensis subsp. novicida U112   Oryza sativa Indica Group   Mus musculus musculus x Mus musculus castaneus   Saccostrea glomerata   Nelumbo nucifera   Pseudomonas composti   Nicotiana glutinosa   Ceratopteris richardii   Palaemonetes sp. common grass shrimp associated circular virus   Mus musculus wagneri   Corynebacterium diphtheriae NCTC 13129   Tilletia caries   Pseudomonas putida F1   Saccharina japonica   Crotalus durissus terrificus   Coxiella-like endosymbiont of Rhipicephalus sanguineus complex   Saccharopolyspora erythraea   Streptomyces sp. EF-93   Aeropyrum pernix   Ruminiclostridium cellulolyticum H10   Frankliniella sp. BIOUG14947-G11   Roseobacter sp. MED193   Butyrivibrio proteoclasticus B316   Moorella thermoacetica ATCC 39073   Streptomyces avidinii   Plasmodium berghei ANKA   Toxoplasma gondii GT1   Streptococcus pneumoniae SP14-BS69   Rhizobium leguminosarum bv. viciae   Selaginella moellendorffii   Larimichthys crocea   Salmonella enterica subsp. enterica serovar Enteritidis str. P125109   Lacticaseibacillus rhamnosus GG   Pachycladon exile   Dokdonia sp. MED134   Pachycladon enysii   Gammarus roeselii   Planctopirus limnophila DSM 3776   Acanthaster planci   Citrullus lanatus   Methylorubrum extorquens DM4   Oryza australiensis   Momordica cochinchinensis   Synechocystis sp. PCC 6803 substr. GT-I   Populus tremula x Populus tremuloides   Nicotiana x sanderae   Phaeodactylum tricornutum CCAP 1055/1   Mycolicibacterium smegmatis   Rhizorhabdus wittichii   Angiostrongylus vasorum   Vipera berus berus   Rickettsia rickettsii   Mycobacterium tuberculosis SP1   Schistocephalus solidus   Pseudomonas aeruginosa LESB58   Vitis riparia   Asterias rubens   Giardia sp.   Tomato spotted wilt orthotospovirus   Lilium   Klebsiella pneumoniae JH1   Flavobacterium sp. 316   Streptomyces sp. MBT84   Scophthalmus maximus   Mycoplasma pneumoniae   Bison   Taenia solium   Lutra lutra   Ensifer adhaerens   Capra pyrenaica   Burkholderia sp. 2332   Sepioloidea lineolata   Paenibacillus polymyxa ATCC 842   Marchantia polymorpha   Frankia alni ACN14a   Rosaceae   Minona ileanae   Mammuthus primigenius   Dialister   Dreissena polymorpha   Botrytis cinerea T4   Drosophila   Campylobacter concisus 13826   Eremothecium gossypii   Pachysolen tannophilus NRRL Y-2460   Hippopotamus amphibius   Andreprevotia sp. IGB-42   Aspergillus tubingensis   Lachancea kluyveri   Enterococcus faecium   Trametes versicolor   Saccharum hybrid cultivar x Sorghum bicolor   Ursus arctos   Burkholderia cenocepacia K56-2Valvano   [Ruminococcus] gnavus   Trichoderma reesei QM9414   Hadronyche infensa   Frankia coriariae   Leishmania braziliensis MHOM/BR/75/M2904   human gut metagenome   Fagus grandifolia   Phytophthora capsici   Bacillus mycoides DSM 2048   Helicobacter pylori 26695   Solenopsis invicta   Rickettsia massiliae MTU5   Willaertia magna   Toxoplasma gondii   Mylodon darwinii   Musa acuminata subsp. acuminata   Scolopendra morsitans   Shigella dysenteriae   Aspergillus carbonarius ITEM 5010   Nicotiana forgetiana   environmental samples   Neospora caninum   Ganoderma lingzhi   Fungi   Nicotiana langsdorffii   Elaeis guineensis   Micrurus tener   Acipenser transmontanus   Fusarium oxysporum Fo47   Candidatus Endoriftia persephone   Blumeria graminis f. sp. tritici   Sparus aurata   Brenneria nigrifluens   Legionella   Emiliania huxleyi   Xanthomonas campestris   Struthio camelus   Pseudomonas aeruginosa UCBPP-PA14   Human immunodeficiency virus   Beta vulgaris   Aspergillus terreus   Knufia petricola   Thermus thermophilus HB8   Gadus morhua   Physcomitrium patens   Escherichia phage vB_EcoP_SU10   Paracoccidioides lutzii Pb01   Legionella pneumophila subsp. pneumophila   Hendra virus horse/Australia/Hendra/1994   Tenebrio molitor   Mycobacterium tuberculosis KZN 1435   Salmonella enterica subsp. enterica serovar Typhimurium str. D23580   Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044   Avicennia officinalis   Blautia producta ATCC 27340 = DSM 2950   Plasmodium falciparum FcB1/Columbia   Naja ashei   Desulfovibrio sp. G11   Emiliania   Epichloe festucae E2368   Natronomonas pharaonis DSM 2160   Polytomella parva   Carica papaya   Sorangium cellulosum So ce56   Veillonella parvula DSM 2008   Vibrio harveyi   Colpophyllia natans   Labeo rohita   Human alphaherpesvirus 1   Cyclotella cryptica   Nicotiana clevelandii   Acanthoscurria geniculata   Gorilla   Alces alces   Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499   Influenza A virus   Daphnia magna   Tarenaya hassleriana   Dasypogon diadema   Nannochloropsis oceanica strain IMET1   Pongo abelii   Naegleria fowleri   Rheum palmatum   Octopodiformes   Anopheles gambiae str. PEST   Bartonella henselae str. Houston-1   Entamoeba histolytica HM-1:IMSS-A   Burkholderia pseudomallei K96243   Mycoplasma hyopneumoniae 232   Bothrops moojeni   Streptococcus suis SC84   Staphylococcus sp. 1   Rhodopseudomonas palustris CGA009   Pinus pinaster   Latrodectus hesperus   Eryx colubrinus loveridgei   Lobaria pulmonaria   Strawberry leafy fruit phytoplasma   Glycine max   Coprococcus sp. ART55/1   Zymoseptoria tritici IPO323   Arachis hypogaea var. vulgaris   Porphyromonas gingivalis W83   Thermoanaerobacter pseudethanolicus   Bacillus amyloliquefaciens DSM 7 = ATCC 23350   Stenotrophomonas maltophilia   Viola tricolor   Rhizophagus irregularis   Naja naja   Mycobacterium tuberculosis CAS   Thermothelomyces thermophilus ATCC 42464   Salmonella enterica subsp. enterica serovar Typhimurium str. A130   Ursus americanus   Cryptococcus neoformans var. grubii H99W   Rhizoctonia solani AG-8 WAC10335   Strongylocentrotus purpuratus   Nicotiana glauca   Geobacillus kaustophilus HTA426   unidentified chimeric sequence   Filifactor alocis   Bacillus subtilis subsp. subtilis   Levilactobacillus brevis   Trichodesmium   Tetrahymena thermophila CU428   Tetrahymena thermophila   Diaphorina citri   Giardia lamblia ATCC 50803   Trypanosoma brucei brucei TREU927   Clostridioides difficile   Corynebacterium glutamicum   Saccharomyces cerevisiae EC1118   Xenopsylla cheopis   Arion vulgaris   Medicago sativa   Fusobacterium nucleatum subsp. nucleatum ATCC 25586   Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344   Aspergillus fumigatus CEA10   Leishmania donovani Ld 2001   prokaryotic environmental samples   Palaemon carinicauda   Lolium perenne subsp. perenne   Citrobacter rodentium   Pygoscelis adeliae   Candidatus Carsonella ruddii DC   Penaeus vannamei   Staphylococcus aureus subsp. aureus   Anas platyrhynchos   Mycoplasma gallisepticum   Synechocystis sp.   uncultured Desulfobacterium sp.   Cuon alpinus   Gorilla gorilla   Trypanosoma brucei   Bacillus thuringiensis Bt407   Bacillus cytotoxicus NVH 391-98   unclassified Marseilleviridae   Nicotiana alata   Nicotiana repanda   Dirofilaria immitis   Oryza longistaminata   Amblyrhynchus cristatus   Nicotiana longiflora   Bodo saltans   Drosophila pseudoobscura   Acheta domesticus   Heligmosomoides polygyrus bakeri   Morchella importuna   Burkholderia ubonensis MSMB22   Plutella xylostella   Human immunodeficiency virus 1   freshwater sediment metagenome   Micrurus fulvius   Picea likiangensis var. rubescens   Aspergillus fumigatus   uncultured soil microorganism   Nicotiana tabacum   Cenchrus americanus   Platichthys flesus   Cucumber mosaic virus   Chromatiales bacterium OalgGamma1   Brochothrix thermosphacta   Dinosauria   Pisum sativum   Hepatitis B virus   Diplazium maximum   Petunia   Bifidobacterium longum NCC2705   Coffea eugenioides   Vairimorpha necatrix   Vitis riparia x Vitis rupestris   Nicotiana africana   Nostoc sp.   Wolbachia endosymbiont of Onchocerca volvulus str. Cameroon   Notamacropus eugenii   Plasmodium knowlesi strain H   Dolichospermum circinale AWQC310F   'Phaseolus vulgaris' phytoplasma   Nostoc sp. PCC 7120 = FACHB-418   Cytomegalovirus   Scutigera coleoptrata   Streptococcus agalactiae   Laxus oneistus   Leishmania mexicana MHOM/GT/2001/U1103   Mycobacterium tuberculosis variant bovis AN5   Moringa oleifera   Chlorobaculum tepidum   Sophora alopecuroides   Mycobacterium tuberculosis H37RvAE   Citrus maxima   Manihot esculenta   Oncorhynchus mykiss   Anopheles culicifacies A   Scheffersomyces stipitis   Streptomyces coelicolor A3(2)   Bacillus subtilis   Sulfolobus islandicus   Catenibacterium   Peniophora incarnata   Streptomyces venezuelae   Erysipelothrix rhusiopathiae   Mycobacterium tuberculosis TKK-01-0032   Tannerella forsythia   Orbicella annularis   Coxiella burnetii   Chenopodium quinoa   Ornithodoros erraticus   Aliivibrio fischeri   Canis lupus x Canis lupus familiaris   Methanosarcina acetivorans   Clostridium perfringens   Arabidopsis   Influenza A virus (A/California/07/2009(H1N1))   Juglans regia   Camponotus fellah   Amphibalanus amphitrite   Allochromatium vinosum DSM 180   Trypanosoma cruzi Dm28c   Cyclopes didactylus   Olavius algarvensis Delta 1 endosymbiont   Salmonella enterica subsp. enterica serovar Typhimurium str. SDT1291   Brachylophosaurus canadensis   Ursus deningeri   Passer domesticus domesticus   Dictyostelia   Flavobacterium columnare   Helicobacter pylori H13-1   Prevotella bryantii B14   Quercus ilex   Riftia pachyptila   Trypanosoma cruzi   Klebsiella pneumoniae BIDMC 40   Human herpesvirus 2 strain 186   Dichocoenia stokesii   Lingulodinium polyedra   Onchocerca ochengi   Enterococcus mundtii CRL35   Ovis canadensis mexicana   Gulo gulo   Acinetobacter baumannii   Phlebotomus papatasi   Schmidtea mediterranea   Bacillus mycoides NBRC 101238 = DSM 11821   Lagomorpha   Ornithodoros moubata   Streptomyces lividans TK24   Echinococcus granulosus   Ascaris suum   Caprinae   Rhodothermus marinus DSM 4252   Betacoronavirus sp.   Crotalus horridus   Neosciurus carolinensis   Oreochromis mossambicus   marine metagenome   Ovis aries   Listeria monocytogenes   Cynodon dactylon   Legionella pneumophila   Plasmodium yoelii   Cladocopium goreaui   Cecropia peltata   Trypanosoma brucei brucei   Brachyspiraceae   Rickettsia slovaca 13-B   Viridiplantae   Anopheles stephensi   Staphylococcus aureus subsp. aureus NN50   Leishmania guyanensis species complex   Plasmodium falciparum   Quercus   Mycobacterium marinum ATCC BAA-535   Acinetobacter calcoaceticus/baumannii complex   Streptomyces sp. MBT42   Mastigamoeba balamuthi ATTC 30984   Hepatitis E virus type 1   Acidithiobacillus ferrooxidans ATCC 53993   Tradescantia spathacea   Francisella orientalis FNO12   Cavia porcellus   Brassica carinata   Tremarctos ornatus   Castor fiber   Escherichia coli O157:H7   Channa striata   Haemonchus contortus   Giardia intestinalis   Brucella abortus S19   Petunia x hybrida   Halobacterium salinarum R1   Marseillevirus   Bothrops neuwiedi   Oscillatoriaceae cyanobacterium   Nicotiana quadrivalvis   Vibrio alginolyticus   Camellia sinensis   Anopheles culicifacies B   Legionella pneumophila str. Paris   uncultured marine microorganism   Tequatrovirus T4   Mycobacterium avium subsp. paratuberculosis K-10   Eucalyptus spp. mixed library   Bicyclus anynana   Crustacea   Bovine leukemia virus   Mammut americanum   Tamias striatus   Mycobacteroides abscessus ATCC 19977   Bothrops jararaca   Agrobacterium tumefaciens   Mustela putorius furo   Dengue virus   environmental samples   Staphylococcus aureus subsp. aureus USA300_FPR3757   Zymomonas mobilis   Cellulophaga baltica NN016038   Aspergillus brasiliensis   Loxodonta africana   Eulimnogammarus cyaneus   Rhipicephalus sanguineus complex sp. ME-2011   Delonix regia   Gossypium robinsonii   Lineus longissimus   Vibrio parahaemolyticus   Staphylococcus haemolyticus   Hydrolagus colliei   Papio hamadryas   Wheat dwarf virus - [Taiyuan]   Phikzvirus phiKZ   Geobacter sulfurreducens   Thermothelomyces heterothallicus   Neospora caninum Liverpool   Hevea brasiliensis   Vulpes lagopus   Clostridium cellulovorans 743B   Sinorhizobium meliloti BL225C   Candida parapsilosis CDC317   Deinococcus deserti VCD115   Streptococcus pyogenes ABC020006030   Drosophila simulans   Sciuridae   Pseudomonas sp. 'Pseudom. M1'   Acesta excavata   Schistosoma haematobium   Paracentrotus lividus   Ciona intestinalis   Yersinia pestis CO92   Leptopilina   Viruses   Telmatactis   Aeropyrum pernix K1   Yersinia enterocolitica   Naja oxiana   Euzebya tangerina   Saiga tatarica   Mycobacterium tuberculosis H37Rv   Heloderma suspectum suspectum   Giardia intestinalis assemblage A   Lynx lynx   Thermosynechococcus vestitus BP-1   Lactococcus lactis subsp. lactis Il1403   Rhodococcus jostii RHA1   Pongo sp.   Amycolatopsis   Calicophoron daubneyi   Chlamydia trachomatis D/UW-3/CX   Caballeronia mineralivorans   Dioscorea alata   Acanthamoeba polyphaga   Latilactobacillus curvatus   Mycoplasma feriruminatoris   Pseudoalteromonas flavipulchra NCIMB 2033 = ATCC BAA-314   Populus tomentosa   Nephotettix cincticeps   Staphylococcus aureus subsp. aureus USA300_TCH1516   mouse gut metagenome   Diplonema papillatum   Pogona vitticeps   Paracoccidioides brasiliensis Pb18   Human alphaherpesvirus 1 strain 17   Solidesulfovibrio magneticus RS-1   mixed culture metagenome   Myxococcus xanthus DK 1622   Microcystis aeruginosa PCC 7806   Paramecium tetraurelia   Pseudodiploria strigosa   Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130   Pectobacterium carotovorum   Gnathostoma spinigerum   Amaranthus tricolor   Agrobacterium fabrum str. C58   Thermococcus kodakarensis KOD1   Dehalococcoides mccartyi CG1   hybrid   Aeromonas sp. enrichment culture clone AM2   Acinetobacter sp.   Vigna mungo   Trichomonas gallinae   Craterostigmus tasmanianus   Gallus   uncultured Phormidium sp.   Cassiopea xamachana   Bacillus cereus ATCC 10987   Fasciola hepatica   Firmicutes   Bithynia siamensis goniomphalos   Bradyrhizobium japonicum   Eimeria tenella   Francisella tularensis subsp. holarctica   Xanthomonas citri pv. glycines str. 8ra   Methanosarcina mazei Go1   Mycobacterium tuberculosis str. Beijing/W BT1   Colinus virginianus   Bactrocera dorsalis   Streptococcus equi subsp. equi   Rhizobium cf. leguminosarum   Thermoclostridium stercorarium   Syntrophus aciditrophicus SB   Beauveria bassiana CGMCC 7.34   Hypsizygus marmoreus   Roseobacter denitrificans OCh 114   Sarcophilus harrisii   Helianthus tuberosus   Acanthamoeba polyphaga mimivirus   Gynandropsis gynandra   glacier metagenome   Aspergillus wentii   Trichodesmium erythraeum IMS101   Faecalibacterium prausnitzii   Helicobacter pylori 26695-1   Olavius algarvensis spirochete endosymbiont   Cupriavidus metallidurans   Thymallus thymallus   Escherichia coli K-12   Candidatus Midichloriaceae   Frankia alni   Staphylococcus pseudintermedius   Bienertia sinuspersici   Chaetoceros sp.   Lactiplantibacillus plantarum JDM1   Streptococcus dysgalactiae subsp. equisimilis SK1249   Sus scrofa   Exiguobacterium sp. SH31   Haemophilus influenzae   Bartonella quintana   Salmonella enterica subsp. enterica serovar Typhimurium str. 5579   Schizosaccharomyces pombe   Norovirus   Geobacter sulfurreducens PCA   Peromyscus leucopus   Bacillus weihenstephanensis   Candidatus Nitrospira inopinata   Boehmeria nivea   Clostridium butyricum E4 str. BoNT E BL5262   Streptococcus uberis   Trypanosoma cruzi strain CL Brener   Capra ibex   Fraxinus nigra   uncultured Clostridium sp.   Acinetobacter baumannii ACICU   Mammalia   Bursaphelenchus xylophilus   Pteropus rodricensis   Laodelphax striatellus   Acinetobacter sp. PM-14   Ceuthophilus sp. Ceu_104_TX_San_Rattlesnake   Rhodopseudomonas palustris   Bradyrhizobium diazoefficiens USDA 110   Ebolavirus   Bacillus thuringiensis serovar berliner ATCC 10792   Oenothera glazioviana   Trypanosoma cruzi cruzi   Burkholderia thailandensis   Trichechus manatus   Piaractus mesopotamicus   Butyrivibrio fibrisolvens   Rosa chinensis   Escherichia coli str. K-12 substr. W3110   Siphoviridae   Leishmania infantum   Aspergillus niger ATCC 13496   Helicobacter pylori A45   Arabidopsis thaliana   Ixodes ricinus   Steinernema carpocapsae   Clostridioides difficile ATCC 43255   Loxosceles intermedia   Daphnia   Pseudomonas sp. Lz4W   Drosophila melanogaster   Odontotaenius disjunctus   Ictalurid herpesvirus 1   Equus caballus   Nitrospirillum amazonense CBAmc   microbial mat metagenome   Chlorocebus aethiops aethiops   Manica rubida   Crunoecia irrorata   Trichinella pseudospiralis   Dendrobium huoshanense   Sedum plumbizincicola   Mycoplasma pneumoniae M129-B7   Camelus dromedarius   Influenza A virus (A/Viet Nam/1203/2004(H5N1))   HIV-1 M:B_HXB2R   Bacillus sp. 142311   Apis mellifera   Haliotis discus hannai   Dichomitus squalens   Brassica oleracea var. gemmifera   Oryzias latipes   Rousettus aegyptiacus   Acanthoscurria gomesiana   Diploria labyrinthiformis   Saccharomyces mikatae IFO 1815   Piscirickettsia salmonis AUSTRAL-005   Eukaryota   Glycine soja   Capreolus capreolus   Acinetobacter baumannii AB5075   Leuconostoc gelidum subsp. gasicomitatum   Chelodina longicollis   Lactobacillus crispatus   Lentinus crinitus   Clostridioides difficile str. A-027   Triticum aestivum   Leucoraja erinacea   Escherichia coli O157:H7 str. TW14359   Pseudomonas sp.   Amphora coffeiformis   Myzus persicae persicae   Syntrophomonas wolfei   Nicotiana maritima   Halomonas sp. HL-48   Pseudodiploria clivosa   Modestobacter marinus BC501   Lactococcus lactis subsp. cremoris MG1363   Podospora anserina S mat+   Cervus nippon hortulorum   Pseudomonas aeruginosa PA14   Oryza sativa Japonica Group   Tilletia controversa   Aspergillus terreus NIH2624   Strigamia maritima   Saimiri boliviensis boliviensis   Lacticaseibacillus rhamnosus   Canis lupus familiaris   Candida tropicalis   Ursus arctos arctos   Broussonetia papyrifera   Bacteroides thetaiotaomicron VPI-5482   Pseudotsuga menziesii var. menziesii   Solanum lycopersicum   Clostridium tetani E88   Lens culinaris   Plasmodium vivax   Prototheca zopfii   Anopheles gambiae   Prochlorococcus marinus subsp. pastoris str. CCMP1986   Pseudomonas syringae   Heterosigma akashiwo   Acromyrmex echinatior   Rhinocerotidae   wastewater metagenome   Cupriavidus basilensis   Lichtheimia corymbifera JMRC:FSU:9682   Bothrops cotiara   Aspergillus   Plasmodium falciparum 58.1   Olavius algarvensis Gamma 3 endosymbiont   Fusarium cf. oxysporum   Pan troglodytes   Agaricus bisporus   Lasius niger   Microbacterium sp.   Noccaea caerulescens   Lentibacter algarum   Yersinia ruckeri   Persicaria minor   Boiga irregularis   Saccharomyces cerevisiae   Tapirus terrestris   Aspergillus nidulans TN02A3   Quercus suber   Acinetobacter baylyi ADP1   Xanthomonas retroflexus   Mus   Sterolibacterium denitrificans   Chlorella sp. SAG 211-18   Streptococcus pneumoniae TIGR4   Bifidobacterium kashiwanohense PV20-2   Caldanaerobacter subterraneus subsp. tengcongensis MB4   gut metagenome   Bothrops jararacussu   Pseudomonas stutzeri   Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S   Chlamydia trachomatis   Rickettsia akari   Dryopteris affinis subsp. affinis   Meyerozyma guilliermondii   Buddenbrockia plumatellae   Candidatus Methylomirabilis oxyfera   Acyrthosiphon pisum   Vulpes vulpes   Bemisia tabaci   Ophrys sphegodes   Aplysia californica   HIV-1 M:G_PT919   Porphyromonas gingivalis   Sitobion avenae   Pleuromamma xiphias   Anaerostipes caccae L1-92   Cutibacterium acnes SK137   Crassostrea hongkongensis   Campylobacter fetus subsp. fetus 82-40   Penicillium sp. B12   Borreliella burgdorferi B31   Bacillus licheniformis   Callinectes sapidus   Avian infectious bronchitis virus (strain Beaudette CK)   Actinomycetia   Riptortus pedestris   Fusarium graminearum   Amblyomma americanum   Listeria monocytogenes EGD   Escherichia coli O157:NM   Beauveria bassiana ARSEF 2860   Streptococcus equi subsp. equi 4047   Pachycladon cheesemanii   Pseudomonas fluorescens   Pyricularia oryzae B157   Moraxella catarrhalis   Olavius algarvensis   Prochlorococcus marinus str. MIT 9313   Vitis   Influenza A virus (H1N1)   Liberibacter endosymbiont of Diaphorina citri   Pseudomonas aeruginosa LESlike1   Mycoplasma pneumoniae 19294   Rhodospirillum rubrum ATCC 11170   Candidatus Kuenenia stuttgartiensis   Dimocarpus longan   Magnaporthe sp. MG05   Enhydra lutris nereis   Pristhesancus sp. 00004635   Tepidanaerobacter acetatoxydans   Solanum lycopersicum x Solanum tuberosum   Rathayibacter toxicus   Haemaphysalis longicornis   Polaribacter marinivivus   Strix varia   Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150   Agkistrodon piscivorus   Pogonomyrmex rugosus   Eubacterium limosum   Toxoplasma gondii ME49   Staphylococcus aureus subsp. aureus str. Newman   Plum pox virus   Rhizopus delemar   Helianthus annuus   Synechococcus sp. PCC 7002   Vaccinia virus   Marchantia polymorpha subsp. ruderalis   Aggregatibacter actinomycetemcomitans D7S-1   Xanthomonas oryzae pv. oryzae PXO99A   Thermococcus eurythermalis   Mycobacterium avium 100   Dionaea muscipula   Mesocricetus auratus   Leishmania braziliensis   Conus betulinus   Sinorhizobium meliloti   Salmonella enterica subsp. enterica serovar Typhimurium   Bacteroides caccae   Paradendryphiella salina   Mycoplasma suis KI3806   Helicoverpa armigera   Sulfuritalea   Mus musculus   Hypsiglena jani   Pseudomonas sp. UK4   Stephanorhinus   Bacillus cereus AH187   Clostridium butyricum DSM 10702   Datura stramonium   Faustovirus   Malaclemys terrapin   Tubastraea coccinea   Austrolebias   Staphylococcus aureus subsp. aureus NCTC 8325   Cajanus cajan   Toxoplasma gondii type III   Lactococcus lactis   Listeria monocytogenes EGD-e   Klebsiella variicola At-22   Parasynechococcus marenigrum WH 8102   Rhodobacter capsulatus SB 1003   Entamoeba histolytica HM-1:IMSS   Thelohanellus kitauei   Chlorella vulgaris   Russula atropurpurea   Simulium damnosum s.l. Me1   Chitinophaga pinensis   rhizosphere soil bacteria ensemble   Dehalococcoides mccartyi   Human alphaherpesvirus 1 strain KOS   Pseudomonas phage EL   Anacardium occidentale   Brugia malayi   Schizosaccharomyces pombe OY26   Olavius algarvensis sulfate-reducing endosymbiont   Caryophyllales   Pseudomonas aeruginosa   Cervus elaphus   Spiroplasma melliferum KC3   Melioribacter roseus P3M-2   Shigella flexneri   Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841   Komagataella pastoris DSMZ 70382   Carnobacterium divergens   Phascolarctos cinereus   Heliocidaris erythrogramma   Actinoplanes sp. SE50/110   Thermodesulfobium acidiphilum   Gorilla gorilla gorilla   Coprothermobacter proteolyticus   Rupicapra pyrenaica   Tetranychus urticae   Quercus petraea   biofilm metagenome   Ctenopharyngodon idella   Gossypium   Lactobacillus   Synechococcus sp. BL107   Bos grunniens   Schizosaccharomyces pombe 927   Apostichopus japonicus   Blastococcus saxobsidens   Papaver somniferum   Sphingobium chlorophenolicum   Nostoc sp. FB71   Halorubrum lacusprofundi ATCC 49239   Lingula anatina   Gallus gallus   Clavibacter michiganensis subsp. michiganensis NCPPB 382   Musa AAB Group   Colon   Leishmania mexicana   Listeria monocytogenes serotype 4b str. F2365   Sinorhizobium americanum CCGM7   Rangifer tarandus   Cyprinus carpio   Helicobacter pylori J99   Spirochaeta sp. ELBA   Cancer   Cercopithecidae   Pyrus x bretschneideri   Pituophis melanoleucus   Capsaspora owczarzaki   Euglena gracilis   Vibrio cholerae O1 biovar El Tor str. N16961   Merluccius bilinearis   Mariprofundus ferrooxydans   Thermoplasma acidophilum DSM 1728   Flavivirus   Dunaliella salina   Human coronavirus HKU1   Biomphalaria glabrata   Granulicatella adiacens ATCC 49175   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819   Bungarus   Aspergillus niger   Methylorubrum extorquens PA1   Drosophila mauritiana   Parasteatoda tepidariorum   Saccharum sp.   Aspergillus flavus   Aeromonas salmonicida subsp. salmonicida   Nicotiana benavidesii   Sepia officinalis   unidentified marine bacterioplankton   Brucella abortus 104M   Bubalus bubalis   Aspergillus nidulans   Bacillus amyloliquefaciens   Morganella   Paramoeba perurans   Mycobacterium tuberculosis C StG4006   Bradyrhizobium   Carnobacterium maltaromaticum   Musa ABB Group   Nerodia clarkii   Volvariella volvacea V23   Candidatus Accumulibacter   Toxoplasma gondii CtCo5   Ovis aries musimon   Sistrurus catenatus   Aspergillus sydowii   Mycobacterium tuberculosis M24   Methanospirillum hungatei JF-1   Klebsiella pneumoniae LCT-KP289   Tuber indicum   Picea abies   Ostreococcus tauri   Tribolium castaneum   Nicotiana benthamiana   Rapana venosa   Hepatitis C virus genotype 1   Acanthamoeba castellanii   Streptococcus pneumoniae   Bacillus cereus   Muvirus mu   Streptococcus thermophilus LMD-9   Pseudomonas taiwanensis   Saccharomyces cerevisiae YJM789   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10   Leishmania tarentolae   Tetrahymena thermophila SB210   Rhodotorula toruloides   Burkholderia cenocepacia H111   phytoplankton environmental sample   Ferroplasma   Orthomyxoviridae   Dactylorhiza   Oryctolagus cuniculus cuniculus   Bradyrhizobium diazoefficiens   Crotalus adamanteus   Severe acute respiratory syndrome coronavirus 2   Aspergillus niger NRRL3   Mytilus edulis   Equus sp.   Human papillomavirus type 16   Alicycliphilus denitrificans BC   Oryctolagus cuniculus   Lactococcus lactis subsp. cremoris UC509.9   Aspergillus flavus NRRL3357   Ophrys garganica   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759   Synechococcus elongatus PCC 7942 = FACHB-805   Methanothermobacter thermautotrophicus   Synechocystis sp. PCC 6803   Corynebacterium diphtheriae   Cucumis sativus   Escherichia coli str. K-12 substr. MG1655   Ectocarpus siliculosus   Cryptococcus neoformans var. grubii H99   Coelodonta antiquitatis   Burkholderia pseudomallei 1026b   Syntrophobacter fumaroxidans MPOB   Cyprinodon variegatus   Leuconostoc gelidum subsp. gelidum   Blumeria hordei DH14   Klebsiella pneumoniae ATCC BAA-2146   Neisseria gonorrhoeae FA 1090   [Candida] glabrata   Cicer arietinum   Sturnus vulgaris   Human herpesvirus 4 type 1   Staphylococcus aureus subsp. aureus COL   Mucilaginibacter   Tursiops truncatus   Francisella tularensis subsp. holarctica LVS   Histomonas meleagridis   Pseudomonas aeruginosa PAO1   Rhipicephalus microplus   Toxoplasma gondii RH   Parhyale hawaiensis   Mayaro virus (strain Brazil)   Avena sativa   Geodermatophilus obscurus DSM 43160   Musa acuminata AAA Group   Leishmania tropica   rat gut metagenome   Ophrys x arachnitiformis subsp. archipelagi   Culex pipiens pipiens   Alteromonadales   Tilletia laevis   Botrytis cinerea B05.10   Synechocystis sp. PCC 6803 substr. Kazusa   Pseudomonas aeruginosa PAO1H2O   Mus musculus x Rattus norvegicus   Nicotiana paniculata   Trichomonas vaginalis   Cleome angustifolia   Roseburia inulinivorans DSM 16841   Cryptosporidium parvum   Amycolatopsis mediterranei S699   Olavius algarvensis Delta 4 endosymbiont   Anser cygnoides   Francisella tularensis   Microcystis aeruginosa   Malassezia sympodialis ATCC 42132   Aspergillus fumigatus Af293   Tyrannosaurus rex   metagenomes   Equisetum arvense   Pinus nigra   Armoracia rusticana   Dictyostelium discoideum AX4   Pogostemon cablin   Paracoccus denitrificans PD1222   Rhynchocyon petersi   Tuber aestivum   Salmonella enterica subsp. enterica serovar Typhimurium str. 14028s Wu   Homo sapiens subsp. 'Denisova'   Mycobacterium avium subsp. paratuberculosis   Naja mandalayensis   unclassified Candidatus Omnitrophica   Myotis ricketti   Schizosaccharomyces pombe 972h-   Pseudo-nitzschia delicatissima   Panthera leo   Komagataella phaffii   Bombyx mori   Apteronotus leptorhynchus   Antidorcas marsupialis   Pisolithus tinctorius Marx 270   Penicillium verrucosum   Acetomicrobium mobile   Sphingomonas sp. Leaf257   Lentimonas   Passer hispaniolensis   Macaca fascicularis fascicularis   Olea europaea   Aggregatibacter actinomycetemcomitans   Toxoplasma gondii GAB3-2007-GAL-DOM9   Strigomonas culicis   Paenibacillus pabuli   Cryptochiton stelleri   Clostridium saccharoperbutylacetonicum   Aspergillus versicolor   Macaca fascicularis   Rattus rattus   Streptococcus thermophilus   Murine leukemia virus   Capsicum annuum   Cyanidioschyzon merolae   Pseudomonas putida   Orycteropus afer   Leptospira interrogans   Mycobacterium tuberculosis variant bovis str. Ravenel   Brachionus plicatilis   Rickettsia conorii subsp. raoultii   Treponema   Formosa sp. Hel3_A1_48   Catalpa fargesii   Apis mellifera pomonella   Candida albicans   Synechococcus cyanophage syn   Acanthochromis polyacanthus   Naja sagittifera   Gammarus fossarum   Brassica rapa   Exidia glandulosa HHB12029   Eucalyptus globulus   Klebsiella pneumoniae MGH 78578   Daphnia pulex   Cancer irroratus   Lactobacillus johnsonii   Desulforamulus reducens MI-1   Salmonella enterica subsp. enterica serovar Typhimurium str. LT2   Glia   Cowpox virus (Brighton Red)   Entamoeba histolytica   Streptococcus mutans 19   Piper nigrum   Enchytraeus crypticus   Litoditis marina   Phytophthora sojae   Ficus carica   Pichia fermentans   Mytilus edulis/galloprovincialis MCLGV-2007   Cryptococcus neoformans var. grubii H99S   Andrias davidianus   Nicotiana bonariensis   Yersinia pseudotuberculosis   Apis mellifera ligustica   Bacteria   Aspergillus aculeatus ATCC 16872   Conus geographus   Pseudomonas aeruginosa PAK   Caenorhabditis elegans   Mycobacterium tuberculosis variant bovis BCG   Clostridium autoethanogenum   Teleogryllus oceanicus   Nicotiana gossei   Taenia hydatigena   sediment metagenome   Colobanthus quitensis   Sulfolobus islandicus REY15A   Tuber albidum   Fusarium oxysporum f. sp. lycopersici 4287   Streptococcus pyogenes M1 GAS   Trypanosoma   Neptuniibacter caesariensis   Sinorhizobium americanum   Tuber melanosporum Mel28   Schizosaccharomyces pombe DM3650   Aves   Populus trichocarpa   Stylosanthes guianensis   Candidatus Profftella armatura   Clavibacter michiganensis subsp. nebraskensis NCPPB 2581   Leishmania chagasi   Plasmodium yoelii yoelii   Puccinellia tenuiflora   Salmonella enterica subsp. enterica serovar Cerro   Prevotella intermedia ATCC 25611 = DSM 20706   Nostoc flagelliforme   Schistosoma japonicum   Castor   Mycoplasma hominis ATCC 23114   Fusarium graminearum PH-1   Leishmania donovani   Neisseria meningitidis FAM18   Trichoderma reesei QM6a   Bacillus subtilis subsp. subtilis str. 168   Oryctolagus cuniculus algirus   Tuber magnatum   Craterostigma plantagineum   Tetrastichus   Mrakia psychrophila   Artemisia annua   Bos indicus   Trypanosoma vivax Y486   Ustilago maydis virus P4   Tetraponera aethiops   Japanese encephalitis virus   Aepyceros melampus   Ustilago maydis   Mycoplasmopsis bovis HB0801   Sorghum bicolor x Saccharum hybrid cultivar   Thermochaetoides thermophila   Argulus foliaceus   Chlorocebus aethiops   Paenibacillus amylolyticus   Nitrososphaera viennensis   Plasmodium falciparum Dd2   Plasmodium berghei   Listeria phage A118   Pseudovibrio sp. FO-BEG1   Pteropus alecto   Fusarium sp.   Ailuropoda melanoleuca   Physalis angulata   Streptococcus pyogenes   Fusarium graminearum GZ3639   Equus hydruntinus   Human herpesvirus 5 strain Merlin   Corynebacterium glutamicum ATCC 13032   Wolbachia endosymbiont of Diaphorina citri   Halobacterium   Staphylococcus aureus subsp. aureus MRSA252   Roseburia hominis A2-183   Kluyveromyces lactis NRRL Y-1140   Candidatus Margulisbacteria   Campylobacter jejuni   Phaeodactylum tricornutum   Serpentes sp. WLS21042014   Brucella   Plasmodium vivax Sal-1   Brucella abortus   Brucella abortus 2308   H1N1 subtype   Dinoroseobacter shibae DFL 12 = DSM 16493   Caretta caretta x Chelonia mydas   Corucia zebrata   Zalophus californianus   Avena strigosa   Pinus taeda   Danio rerio   Dehalococcoides mccartyi CBDB1   Ricinus communis   Salmonella bongori   Octopus vulgaris   Madurella mycetomatis   Meandrina meandrites   Cricetulus griseus   Penicilliopsis zonata   Coronavirus PREDICT CoV-2   Mycoplasmopsis agalactiae   Phytophthora infestans   Ipomoea batatas   Bartonella quintana JK 31   Saccharomyces cerevisiae BY4741   Bothrops insularis   Bothrops brazili   Archaea   Bacillus anthracis str. Sterne   Lithobius forficatus   Vitis labrusca x Vitis riparia   Ruminiclostridium papyrosolvens DSM 2782   Nothobranchius furzeri   Gasterosteus aculeatus   Populus yunnanensis   Dentex dentex   Bacillus cereus ATCC 14579   West Nile virus   Equus asinus africanus   Brucella anthropi ATCC 49188   Mycoplasma gallisepticum S6   Calvadosia cruxmelitensis   Microcystis aeruginosa NIES-843   Dioscorea oppositifolia   Bordetella pertussis   Megoura viciae   Oryza sativa   Schizophyllum commune H4-8   Brachypodium distachyon   Deltaproteobacteria bacterium RIFCSPLOWO2_12_FULL_50_11   Listeria monocytogenes HCC23   Rhizobium favelukesii   Trichoderma sp. 1 YW-2011   Agkistrodon contortrix   Lactococcus phage   Streptococcus pyogenes STAB901   Vespula vulgaris   Hominidae   Mycobacterium marinum   Acinetobacter baumannii D1279779   Paulownia   Bacillus toyonensis BCT-7112   Mammuthus sp.   Pongo pygmaeus   Salmonella enterica subsp. enterica serovar Typhi str. CT18   Venturia inaequalis   Xenopus laevis   marine plankton environmental sample   Ictalurus punctatus   Mycoplasma pneumoniae M29   Desmodesmus subspicatus   Sistrurus miliarius barbouri   Tritrichomonas foetus   Myxobolus honghuensis   Breviolum minutum   Mycobacterium tuberculosis   Nitrospinae   uncultured bacterium   Mahonia   Opsanus beta   Myxobolus wulii   Phytophthora pisi   Epstein-barr virus strain p3hr-1   Yarrowia lipolytica   Pseudomonas aeruginosa str. E2UoS   Meles meles   Jatropha curcas   Fomitopsis pinicola   Panopea generosa   Fragaria x ananassa   Nematostella vectensis   cf. Chlorella sp.   Candidatus Thiosymbion oneisti   Influenza A virus (A/Wilson-Smith/1933(H1N1))   Thermococcus gammatolerans EJ3   Gardnerella vaginalis   Bos taurus   Stappia stellulata DSM 5886   Mentha spicata   Polypodium hydriforme   Medicago truncatula   Liquorilactobacillus nagelii   Sanghuangporus   Dictyostelium discoideum   Chlamydia trachomatis 434/Bu   Coxsackievirus B3   Escherichia coli O157:H7 str. EDL933   Serratia inhibens PRI-2C   Granulicella sp.   Conus striatus   Herpes simplex virus unknown type   Lactobacillus iners   Mytilus sp. CHGF4   Myodes glareolus   Triticum turgidum subsp. durum   Komagataella pastoris   Glycine max x Glycine soja   Mycobacterium tuberculosis SP7   Haloferax volcanii DS2   Thalassiosira cf. oceanica   Bombus terrestris   Methermicoccus shengliensis DSM 18856   Leishmania donovani BPK282A1   Colobus guereza   environmental samples   Nicotiana rustica   Aedes albopictus   Eimeria acervulina   Solanum tuberosum   Pachycladon novaezelandiae   Ixodes scapularis   Chaetodon lunulatus   Passiflora edulis   Pyricularia oryzae P131   Campylobacter concisus UNSWCD   Capra hircus   Dolichospermum circinale AWQC131C   Aerococcus urinae NBRC 15544 = CCUG 36881   Python molurus   Dendroaspis angusticeps   Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821   Cloning vector pEGFP-C1   Thalassiosira pseudonana CCMP1335   Paucilactobacillus suebicus   Dendroaspis polylepis polylepis   Staphylococcus cohnii   Olavius algarvensis Gamma 1 endosymbiont   Vibrio anguillarum   Ictidomys tridecemlineatus   Pseudoalteromonas sp. D41   Pseudomonas putida BIRD-1   Staphylococcus aureus   Gossypium hirsutum   Acinetobacter baumannii AB307-0294   Chara australis   Staphylococcus xylosus   Candidatus Thioglobus autotrophicus   Brassica napus   Tetronarce californica   Triaenophorus crassus   Anguilla anguilla   Oreochromis niloticus niloticus   Secale cereale   Hordeum vulgare   Novosphingobium nitrogenifigens   Bacillus pseudomycoides DSM 12442   Acholeplasma laidlawii PG-8A   Oleidesulfovibrio alaskensis G20   Coxiella burnetii RSA 493   Populus cathayana   Ophiophagus hannah   Vibrio cholerae O1 str. VC91   Bombus terrestris audax   Panine betaherpesvirus 2   Trypanosoma cruzi strain Esmeraldo   Trifolium pratense subsp. pratense   Aspergillus giganteus   Methanohalophilus   Bifidobacterium pseudolongum   Oomycota   HIV-1 vector pNL4-3   Nostoc punctiforme PCC 73102   Potentilla saundersiana   Vitis hybrid cultivar   Mycobacterium tuberculosis SP10   Actinidia chinensis var. chinensis   Crassostrea gigas   Leptospirillum   Bothrops leucurus   Zygaena filipendulae   Hippophae rhamnoides   Modiolus kurilensis   Durusdinium trenchii   Priestia megaterium DSM 319   Rhodnius prolixus   Medicago truncatula var. truncatula   Pomacea maculata   Oenococcus oeni   Streptococcus mutans UA159   Pseudomonas ogarae   Perkinsus marinus   Physcomitrella   artificial sequences   Theobroma cacao   Nephila clavipes   Harmonia axyridis   Euterpe oleracea   Rattus norvegicus   Rhizobium leguminosarum bv. phaseoli CCGM1   Enterobacter cloacae   Salmo sp.   Heterorhabditis megidis   Helicobacter pylori E48   (Vitis vinifera x Vitis berlandieri) x Vitis berlandieri   Leishmania infantum JPCM5   Aspergillus flavus partitivirus 1   Leishmania major strain Friedlin   Synechococcus sp. YX04-3   Staphylococcus aureus subsp. aureus USA300   Burkholderia cenocepacia   Panicum virgatum   Musa   Caulobacter vibrioides NA1000   Rhizobium sp. CIAT652   Vicia faba   Eriocheir sinensis   Dugesia japonica   Bivalvia   Akkermansia muciniphila ATCC BAA-835   Mycobacterium tuberculosis SP22   Cupiennius salei   Pseudomonas putida KT2440   Bordetella parapertussis   Escherichia coli Nissle 1917   Cairina moschata   Strongyloides venezuelensis   Aedes aegypti densovirus   Trichoplax sp. H2   Escherichia coli BL21(DE3)   Meleagris gallopavo   albimanus section   Photobacterium profundum SS9   Prochlorococcus marinus subsp. marinus str. CCMP1375   Quercus robur   Lotus japonicus   Cellvibrio japonicus Ueda107   bioreactor sludge metagenome   Aedes aegypti aegypti   Mycobacterium tuberculosis H37Rv_2009   Limosilactobacillus mucosae LM1   Gammarus pulex   Fibrobacter succinogenes subsp. succinogenes S85   Fusarium oxysporum f. sp. cucumerinum   Hydra vulgaris   Brassica oleracea var. botrytis   Sordaria macrospora   Haptophyta   Aspergillus aff. niger A2   Shigella sonnei 53G   Sinopodophyllum hexandrum   environmental samples   Spondylus gaederopus   Brucella melitensis   Equus asinus   Neurospora crassa   Marthasterias glacialis   Xanthomonas translucens   Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14   Megaloceros giganteus   Acinetobacter baumannii ATCC 17978   Enterococcus faecalis   Euprymna scolopes   Orbicella faveolata   Corynebacterium diphtheriae bv. mitis str. ISS 3319   Desulfovibrio ferrophilus   Murine leukemia virus isolates   Erysipelatoclostridium ramosum DSM 1402   Clostridioides difficile R20291   Staphylococcus lugdunensis   Phaseolus vulgaris   human metagenome   Trichoderma reesei RUT C-30   Desulforapulum autotrophicum HRM2   Botryococcus braunii   Haemophilus haemolyticus   soil bacterium TS1114R   Ralstonia solanacearum GMI1000   Streptococcus pyogenes MGAS5005   Felis catus   Oncorhynchus tshawytscha   Escherichia coli   Candidatus Accumulibacter phosphatis   Paenibacillus sp.   Halobacterium salinarum   Cervus nippon nippon   Lymnaea stagnalis   Montastraea cavernosa   Chlorella sp. FC2 IITG   Galleria mellonella   Aspergillus niger CBS 513.88   Agrostis stolonifera   Martes martes   Legionella pneumophila subsp. pneumophila str. Philadelphia 1   Dickeya dadantii subsp. dadantii   Corynebacterium glutamicum K051   Syntrophus aciditrophicus   Secale cereale x Triticum turgidum subsp. durum   Aiptasia   Streptococcus pneumoniae D39   Mucidula mucida   Plasmodium falciparum 3D7   Sus scrofa domesticus   Ruegeria pomeroyi   Lactiplantibacillus plantarum subsp. plantarum   Tropilaelaps mercedesae   Staphylococcus epidermidis   Liquorilactobacillus hordei   Aedes aegypti   Mycoplasma pneumoniae M129   Viola odorata   Globicatella sanguinis   Brassica oleracea   Streptococcus agalactiae FSL S3-026   Crocosphaera subtropica ATCC 51142   Influenza B virus (B/Memphis/13/2003)   Candidatus Paraburkholderia kirkii   Streptomyces sp. MC1   Sorghum bicolor   Phaeobacter sp. MED193   Triatoma sordida   Arbacia punctulata   Spirometra erinaceieuropaei   Morganella   Haloferax volcanii   Camelidae   Venturia pyrina   Alligator mississippiensis   Camponotus floridanus   Methanoculleus sp. 10   Mycobacteroides abscessus   Lysiphlebus fabarum   Anoplocephala perfoliata   Pyrococcus furiosus   Shigella flexneri 2a str. 2457T   Ustilaginoidea virens   Streptomyces coelicolor   Porphyromonas gingivalis ATCC 33277   Microbiota   Orthopoxvirus   Pseudomonas pseudoalcaligenes CECT 5344   Cricetulus barabensis   Mytilus galloprovincialis   Synechococcus phage S-SSM7   Shewanella oneidensis MR-1   Saccharum hybrid cultivar   Pyrococcus abyssi   Cryptosporidium andersoni   Luteibacter   Schistosoma mansoni   Arachis hypogaea   Francisella tularensis subsp. holarctica FSC200   Rupicapra rupicapra   Fusarium oxysporum f. sp. lycopersici   Plasmodium sp. pBT9   Amblyomma cajennense   Shewanella baltica   Chlamydomonas reinhardtii   Clostridioides difficile 050-P50-2011   [Candida] glabrata CBS 138   Sarcoptes scabiei   Angiostrongylus cantonensis   uncultured microorganism   Onchocerca volvulus   Ianthella basta   Bacillus pumilus   Zea mays   Daktulosphaira vitifoliae   Chlorocebus sabaeus   Ovis   Alternaria tomato   Ictidomys tridecemlineatus arenicola   Viola tricolor subsp. tricolor   Sulfolobus islandicus LAL14/1   Leishmania major   Streptococcus suis   Thermus thermophilus   Pachycladon fastigiatum   Brassica oleracea var. italica   Tepidimicrobium xylanilyticum   Tilletia indica   Clostridioides difficile 630   Trichinella britovi   environmental samples   Cyanophora paradoxa   Pseudoliparis swirei   Afipia carboxidovorans   Axis axis   Mycolicibacterium smegmatis MC2 155   Nelumbo nucifera subsp. nucifera   Myxobilatus gasterostei   Synechococcus elongatus   Azospirillum brasilense FP2   Pinus radiata   Nanger dama mhorr   Synechococcus phage syn9   Neisseria gonorrhoeae   Sulfolobus acidocaldarius   Malus domestica   synthetic construct   Candidatus Protochlamydia amoebophila UWE25   Acidipropionibacterium acidipropionici   Toxoplasma gondii PRU   Nicotiana plumbaginifolia   Panthera tigris   Klebsiella pneumoniae   [Candida] auris   Pasteurella multocida subsp. multocida str. Pm70   Homo   Osphranter rufus   Toxoplasma   Deinococcus radiodurans R1   Thalassiosira pseudonana   Botrytis cinerea   Mustela sp.   Homo sapiens/Mus musculus xenograft   Crocuta crocuta   Pseudotsuga menziesii   Macadamia   Herbaspirillum seropedicae   unidentified   Myzus persicae   Wolbachia sp. wMelPop   Mycobacterium tuberculosis LAM7   Aspergillus oryzae var. oryzae   Hyalella azteca   Saccharomyces cerevisiae W303   Prochlorococcus marinus str. MIT 9312   Candidatus Pelagibacter ubique   Ursus maritimus   Mycobacterium tuberculosis SP13   Spiroplasma melliferum   Mengo virus   Clostridium cellulovorans   Human herpesvirus 5 strain AD169   Macaca mulatta   Metopolophium dirhodum   Afrohybanthus enneaspermus   Beta vulgaris subsp. vulgaris   Kluyveromyces marxianus   Arvicola   environmental samples   Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940   Ambystoma mexicanum   Populus tremula x Populus alba   Renibacterium salmoninarum ATCC 33209   Citrus sinensis   Pomacea canaliculata   Roseburia intestinalis L1-82   Metarhizium robertsii   Nicotiana noctiflora   Candida albicans SC5314  

TISSUE(S): Epithelial Cell

SUBMITTER: Kathrin Nowak  

LAB HEAD: Michael O. Hottiger

PROVIDER: PXD016686 | Pride | 2020-10-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
10mg_eAf_1_EThcD.csv Csv
10mg_eAf_1_HCD.csv Csv
10mg_eAf_2_EThcD.csv Csv
10mg_eAf_2_HCD.csv Csv
10mg_eAf_3_EThcD.csv Csv
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Publications


Protein ADP-ribosylation is a reversible post-translational modification that regulates important cellular functions. The identification of modified proteins has proven challenging and has mainly been achieved via enrichment methodologies. Random mutagenesis was used here to develop an engineered Af1521 ADP-ribose binding macro domain protein with 1000-fold increased affinity towards ADP-ribose. The crystal structure reveals that two point mutations K35E and Y145R form a salt bridge within the A  ...[more]

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