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ProteomicsAS precipitation and protein A affinity chromatography for abundant plasma protein depletion
Plasma is a highly valuable resource for biomarker research since it is easy obtainable and contains a high amount of information on patient health status. Although advancements in the field of proteomics enabled analysis of the plasma proteome, identification of low abundant proteins remains challe...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-08 | PXD008969 | Pride
Plasma Mouse Abundant protein depletion 
ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-17 | MSV000083252 | MassIVE
ProteomicsDNA-protein affinity precipitation to identify proteins interacting with the atpT promoter in Synechocystis sp. PCC 6803
The small protein AtpΘ (encoded by gene atpT) identified in cyanobacteria becomes maximum expressed during low-energy conditions such as during darkness. In the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803), AtpΘ was demonstrated to inhibit the ATP hydrolysis activity of the F...
ORGANISM(S): Synechocystis sp. PCC 6803 
2022-04-14 | PXD027801 | Pride
Dna-protein affinity precipitation  atpt promoter  synechocystis sp. pcc 6803  cyabrb 
TranscriptomicsChip-chip of Sir3 and of Sir2, and ChAP-chip of AAR from budding yeast cells
O-acetyl-ADP-ribose (AAR) is a small metabolic molecule that is generated during NAD-dependent deacetylation by Sir2. Sir2 regulates gene expression, DNA repair, and genome stability. chromatin affinity-precipitation (ChAP) method was used to detect the chromatin fragments at which small molecules i...
ORGANISM(S): Saccharomyces cerevisiae 
2015-12-31 | E-GEOD-65672 | biostudies-arrayexpress
UnknownAffinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand.
Not available
S-EPMC3903448 | biostudies-literature
TranscriptomicsChromosomal High Affinity Binding Sites for the Drosophila Dosage Compensation Complex.
We identified 131 high affinity sites of the Drosophila DCC combining residual ChIP-chip profiles after differential crosslinking and RNAi-mediated knockdown of spreading factors Keywords: ChIP-chip MSL1 and MSL2 ChIP under various conditions including differential crosslinking and RNAi against MOF...
ORGANISM(S): Drosophila melanogaster 
2008-12-13 | E-GEOD-12292 | biostudies-arrayexpress
UnknownStable and reusable calcium-responsive biopolymer for affinity precipitation of therapeutic antibodies.
Not available
S-EPMC11285868 | biostudies-literature
TranscriptomicsChromatin Affinity Precipitation-chiop of B. subtilis to gain insight into the invivo dynamics of RNA polymerase
To obtain an insight into the in vivo dynamics of RNA polymerase (RNAP) on the B. subtilis genome, we analyzed the distribution of ?A and ? subunits of RNAP and the NusA elongation factor on the genome in exponentially growing cells, using the ChAP (Chromatin Affinity Precipitation)-chip method. In ...
ORGANISM(S): Bacillus subtilis 
2010-06-29 | E-MEXP-2649 | biostudies-arrayexpress
GenomicsEvaluation of affinity-based genome-wide DNA methylation data: effects of CpG density, amplification bias and copy number variation
Abstract: DNA methylation is an essential epigenetic modification that plays a key role associated with the regulation of gene expression during differentiation but in disease states such as cancer, the DNA methylation landscape is often deregulated. There are now numerous technologies available to...
ORGANISM(S): Homo sapiens 
2010-10-08 | E-GEOD-24546 | biostudies-arrayexpress
TranscriptomicsIdentification of high-affinity microRNA targets
We report that microRNAs strongly regulate targets of exceptionally high affinity and that such targets can be identified upon microRNA silencing in Argonaute 2 ribonucleoprotein immunoprecipitation (Ago2-RIP) experiments
ORGANISM(S): Mus musculus 
2017-08-03 | E-MTAB-5386 | biostudies-arrayexpress
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