Sort   by:  
 Page size 
ProteomicsSMARCD1 Affinity Purification-mass Spectrometry (AP-MS) in HEK-293 Cells
This study reports the identification of the FLAG-tagged SMARCD1 protein interactome in HEK-293 cells using Affinity Purification-Mass Spectrometry (AP-MS). The data provides a comprehensive list of proteins that physically associate with SMARCD1, thereby contributing to the understanding of its mol...
ORGANISM(S): Homo sapiens (Human) 
2026-07-09 | PXD068854 | Pride
Ap-ms Smarcd1 
ProteomicsAPPLE-MS: A modular affinity purification-mass spectrometry method assisted by PafA- mediated proximity labeling
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-10-27 | PXD063145 | Pride
Ap-ms Proximity labeling Protein-protein interaction Interactome 
ProteomicsIn-vivo crosslinking based affinity purification-mass spectrometry for targeting intracellular protein-protein interactions
Comprehensive interactome analysis of targeted proteins is of great significance to understand how pro-teins work together for functional regulation. Until now, affinity purification strategy with mass spec-trometry (AP-MS) has been recognized as the most widely used technique for studying protein-p...
ORGANISM(S): Homo Sapiens 
2022-12-01 | PXD038473 |
ProteomicsHighly accurate protein complex retrieval by affinity enrichment MS rather than affinity purification MS
Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identifi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-03-26 | PXD000955 | Pride
Ap-ms Ae-ms Yeast Interaction proteomics Label-free quantification 
UnknownAccurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).
Not available
S-EPMC4288248 | biostudies-literature
ProteomicsIsolation of aphid-Potato leafroll virus (PLRV) proteins complexes from viruliferous insects using affinity purification-mass spectrometry
The control of insect borne disease is recognized as one of the major agricultural, animal and human health challenges of today. Viruses in the family Luteoviridae are phloem-limited, plant viruses that are vectored by aphids in a circulative manner. They are responsible for a wide-range of economic...
ORGANISM(S): Potato leafroll virus Myzus persicae 
2021-05-27 | PXD022167 | Pride
Aphid Phloem-limited plant virus Ap-ms. Potato pathogen Potato leafroll virus Plrv Luteoviridae Polerovirus Circulative transmission Myzus persicae 
ProteomicsSEPT6 affinity purification-mass spectrometry (AP-MS)
Septin proteins interact intermolecularly to form hetero-oligomeric complexes that further assemble into higher-order filaments. To investigate whether the interactions among septin proteins are affected by RID, we conducted affinity purification-mass spectrometry (AP-MS) experiments. In this regard...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD046497 | Pride
Sept6 Rid Fatty-acylation 
ProteomicsIdentify the interacting proteins of FBXL4 by AP-MS
Affinity purification coupled with mass spectrometry (AP-MS) is powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex.Mass spectrometry was performed to identify the interacting proteins of FBXL4 fro...
ORGANISM(S): Homo sapiens (Human) 
2024-05-12 | PXD040601 | Pride
Human Fbxl4 Ap-ms 
ProteomicsBait correlation improves interactor identification by TMT-AP-MS
The quantitative multiplexing capacity of isobaric Tandem Mass Tags (TMT) has increased the throughput of affinity purification mass spectrometry (AP-MS) to characterize protein interaction networks of immunoprecipitated baits. However, variable bait levels between replicates can convolute interacto...
ORGANISM(S): Homo sapiens (Human) 
2020-06-22 | PXD016613 | Pride
Tmt Protein-protein interactions Ap-ms 
ProteomicsiMixPro: intelligent Mixing of Proteomes for elimination of false positives in affinity purification-mass spectrometry
Protein complexes are essential in most organizational and functional aspects of the cell. Different strategies currently exist to analyze such protein complexes by mass spectrometry, including affinity purification (AP-MS) and proximal labeling based strategies. However, the high sensitivity of cur...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD004246 | Pride
Human overexpression cell lines ap-ms silac 
Sort   by:  
 Page size