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CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.
A non-toxic, abscisic acid-based anchor-away system for efficient nuclear depletion of budding yeast proteins
We obtained RNA polymerase II occupancy profile across the genome of S.cerevisiae in several strains, Wild type at 25 and 37 degrees C, RNA15-1 at 25 and 37 degrees C, anchor-away parental strain plus rapamycin, Reb1 anchor-away plus rapamycin, Rap1 anchor-away plus rapamycin, Nrd1-AID plus auxin, a...
ORGANISM(S): Saccharomyces cerevisiae 
2018-01-10 | GSE97913 | GEO
Genome-wide measurement of H3K4me3 and H3K36me3 levels following depletion of RNAPII from the nucleus via an anchor-away system.
We obtained RNA polymerase II occupancy profiles across the genome of S.cerevisiae strains: Abf1 anchor-away cells after addition of rapamycin for different time points (including no addition of rapamycin) and Rap1-AID auxin-degron cells after addition of auxin for different time points (including n...
ORGANISM(S): Saccharomyces cerevisiae 
2018-12-20 | GSE114589 | GEO
With the aim of overcoming some of the problems associated with yeast genetic depletion systems, we developed a modified anchor-away (AA) system that relies on a chemically induced dimerization system involving the plant phytohormone abscisic acid (ABA). An advantage of employing this CID in an anch...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2025-11-22 | GSE149746 | GEO
Nuclear depletion of Mot1 and NC2beta by anchor-away reveals cooperation in vivo to regulate TBP function and gene expression
ORGANISM(S): Saccharomyces cerevisiae 
CRAC of yeast RNA polymerase II in various thermosensitive strains at permissive and non-permissive temperature and anchor-away strains with the addition of rapamycin.
We measured the transcriptome of yeast cells during the depletion of chromatin regulators using anchor away
ORGANISM(S): Saccharomyces cerevisiae 
2023-06-29 | GSE234023 | GEO
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