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TranscriptomicsHigh-throughput SuperSAGE for digital gene expression analysis of multiple samples using Next Generation Sequencing
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index...
ORGANISM(S): Danio rerio 
2010-09-30 | E-GEOD-20682 | biostudies-arrayexpress
GenomicsLEM-2 ChIP-seq to study chromatin anchoring
Chromatin Immunoprecipitation against the nuclear envelope protein LEM-2 to detect association to the nuclear periphery. LEM-2 ChIP was performed on early embryo fixed extracts for wild-type and two mutants that have altered position of a perinuclear chromatin reporter. LEM-2 ChIP on early embryo ch...
ORGANISM(S): Caenorhabditis elegans 
2015-12-11 | E-GEOD-74133 | biostudies-arrayexpress
TranscriptomicsRNA-seq for monitoring expression changes in absence of chromatin anchoring
RNA-seq for monitoring expression levels in mutants that do not anchor chromatin at the nuclear periphery. RNA-seq of depleted rRNA samples of early embryo extracts for three different genotypes: wild-type, cec-4_delta and met-2 set-25_delta_delta, in two independent biological replicas
ORGANISM(S): Caenorhabditis elegans 
2015-12-11 | E-GEOD-74067 | biostudies-arrayexpress
GenomicsPerinuclear anchoring of H3K9-methylated chromatin stabilizes induced cell fate
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Caenorhabditis elegans 
2015-12-11 | E-GEOD-74134 | biostudies-arrayexpress
GenomicsGAS41 modulates ferroptosis by anchoring NRF2 on chromatin
GAS41 modulates ferroptosis by anchoring NRF2 on chromatin
PRJNA1079732 | ENA
GenomicsPhosphorylation of Barrier-to-Autointegration Factor (BAF) regulates anchoring of centromeric heterochromatin to the nuclear envelope (NE)
Phosphorylation of Barrier-to-Autointegration Factor (BAF) regulates anchoring of centromeric heterochromatin to the nuclear envelope (NE)
PRJNA1268542 | ENA
TranscriptomicsAAV2-GFP-Calcineurin anchoring disruptor microarray
We have analyzed how interaction of calcineurin with perinuclear signalosome AKAP6 affects the expression profile of transcription factors involved in cAMP/calcium signaling. We have found that calcineurin interacts with AKAP6 in calcium-dependent manner and by using anchoring disruptors, we demonst...
ORGANISM(S): Rattus norvegicus 
2024-12-12 | E-MTAB-14614 | biostudies-arrayexpress
GenomicsThe ER Anchoring and Abundance of the EEF1B Complex is Affected by Tissue Specific Alternative EEF1D Spicing
The ER Anchoring and Abundance of the EEF1B Complex is Affected by Tissue Specific Alternative EEF1D Spicing
PRJNA1438049 | ENA
GenomicsMammalian PERIOD complex regulates repair of DSB in active chromatin through anchoring to the nuclear envelope
Mammalian PERIOD complex regulates repair of DSB in active chromatin through anchoring to the nuclear envelope
PRJEB101600 | ENA
UnknownSingle nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins.
Not available
S-EPMC6298107 | biostudies-literature
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