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CD28 region-capture HiC of resting and anti-CD3/CD28-stimuated Jurkat cells
ChIP-seq of resting and anti-CD3/CD28-stimuated Jurkat cells
4C-seq of resting and anti-CD3/CD28-stimuated Jurkat cells
Peripheral blood lymphocytes were separated in the Ficoll gradient and subjected for stimulation with anti-CD3 and anti-CD28 antiobodies upon time (6h, 12h and 18h). Next, total RNA was isolated and trenscriptional analysis of stimulated cells was performed. 11 total samples were analysed. Analysis ...
ORGANISM(S): Homo sapiens 
ATAC-seq of resting and anti-CD3/CD28-stimuated Jurkat cells
RNA-seq of resting and anti-CD3/CD28-stimuated Jurkat cells
To compare the transcriptomes of T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated using panT-cell kit in an AutoMACS device (Miltenyi). Cells were cultured with anti-CD3 plus anti-CD28 antibodies (2 µg antibody/ml) for th...
ORGANISM(S): Mus musculus 
RNA-Seq of T cells stimulated with anti-CD3/CD28 for 4 h
PBMCs were isolated from whole blood taken from one patient and one healthy control. Cells were cultured in media (RPMI) alone, with 1µg/ml anti-CD3, with 1µg/ml anti-CD3 & 1µg/ml anti-CD28 or with 0.5ng/ml LPS. Cells were harvested after 24 or 48 hours, RNA isolated using Trizol, purified using R...
ORGANISM(S): Homo sapiens 
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