Transcription profiling of mouse T-cells from wildtype and Gfi1 knockout mice activated by anti-CD3 plus anti-CD28 for 0 to 24h

ABSTRACT: To compare the transcriptomes of T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated using panT-cell kit in an AutoMACS device (Miltenyi). Cells were cultured with anti-CD3 plus anti-CD28 antibodies (2 µg antibody/ml) for the indicated time period. Total RNAs was isolated and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays. Experiment Overall Design: 8 samples, 1 replicate per group

ORGANISM(S): Mus musculus

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-4940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress