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GenomicsAn interactive database for the assessment of histone antibody specificity
Commercially available histone antibodies were analyzed using peptide microarrays to assess specificity. For two H3K27me3 antibodies from different vendors, we performed ChIP-seq in WT and EED-null murine ES cells to assess specificity. Cell lines were generated from e3.5 blastocysts of EED fl/fl an...
ORGANISM(S): Mus musculus 
2015-06-21 | E-GEOD-66902 | biostudies-arrayexpress
ProteomicsIntact Transition Epitope Mapping- Thermodynamic Weak-force Order (ITEM-TWO)
Most important characteristics of antibodies are that they typically strongly bind to specific epitopes, thereby expressing low dissociation constants (KDs) and high Gibbs free binding energies (delta Gs). In this study, we describe an off-line nano-electrospray mass spectrometry method, termed IT...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD016024 | Pride
Antibody affinity determination Antibody specificity determination 
ProteomicsDetermination of specificity of the MAbs to Der p 21
For determination of specificity of the MAbs, rDer p 21, thermally denatured rDer p 21 and rMBP-Der p 21 (0.3 mg/mL) were printed. the slides were incubated for 2 h 5 different MAbs (0.5 µg/mL for specificity analysis). Then the slides were incubated for 30 min goat anti-mouse IgG Fc Alexa Fluor® 6...
ORGANISM(S): Mus musculus 
2023-12-25 | E-MTAB-13625 | biostudies-arrayexpress
GenomicsAsymmetry of STAT action in driving IL-27 and IL-6 transcriptional outputs and cytokine specificity
IL-6 and IL-27 have antagonistic and overlapping functions, signal through a shared receptor subunit and employ the same downstream STAT proteins. To evaluate the degree of specificity and redundancy for these cytokines, we quantified global transcriptomic changes induced by the two cytokines and de...
ORGANISM(S): Mus musculus 
2015-05-27 | E-GEOD-65621 | biostudies-arrayexpress
ProteomicsThe specificity of the anti-bass IgM monoclonal antibody (mAb) was validated by mass spectrometry.
The specificity of the anti-bass IgM monoclonal antibody (mAb) was rigorously validated by immunoprecipitation coupled with mass spectrometry (IP-MS). This analysis confirmed the mAb’s selective binding to IgM while identifying potential off-target interactions, ensuring its suitability for IgM depl...
ORGANISM(S): Micropterus Salmoides 
2025-05-02 | PXD063688 |
TranscriptomicsRNA-seq reveals that Air1 and Air2 control TRAMP substrate specificity for nuclear RNA surveillance
The Saccharomyces cerevisiae TRAMP4 and TRAMP5 complexes, which consist of the poly(A) polymerase Trf4 or Trf5, respectively, the zinc knuckle proteins Air1 or Air2, and the RNA helicase Mtr4, play a critical role in nuclear RNA surveillance. Although it is known to enhance the nuclease activity of ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-11-14 | E-GEOD-42231 | biostudies-arrayexpress
TranscriptomicsThe tinkerbell (tink) mutation identifies the dual-specificity MAPK phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) as a novel regulator of organ size in Arabidopsis.
Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK ph...
ORGANISM(S): Arabidopsis thaliana 
2015-07-30 | E-GEOD-66419 | biostudies-arrayexpress
TranscriptomicsSmall Marine Neurotoxin Elicits Common Antibody Response in Zebrafish and Sea Lions
The neurotoxic amino acid, domoic acid, is naturally produced by marine phytoplankton and presents a significant health threat to marine mammal and human populations. Currently, diagnostic tools to assess exposure are not available, yet concerns regarding health impacts associated with low-level re...
ORGANISM(S): Danio rerio 
2013-01-03 | E-GEOD-34716 | biostudies-arrayexpress
TranscriptomicsAntibody repertoire RNA-seq throughout key stages of mouse B-cell development (pre-B-cells, naive B cells, plasma cells)
We sought to investigate the extent of genetic and antigen-driven predetermination of antibody repertoires throughout B-cell development (pre-B cells, naive B cells, plasma cells). To this end, we used (i) an inbred model organism (mouse, C57BL/6J) to quantify genetic predetermination and (ii) cohor...
ORGANISM(S): Mus musculus 
2017-03-28 | E-MTAB-5349 | biostudies-arrayexpress
ProteomicsMultiplex assay for quantification of acute phase proteins and immunoglobulin A in dried blood spots
Inflammation is the first line defense mechanism against infection, tissue damage or cancer development. However, inappropriate inflammatory response may also trigger diseases. The quantification of inflammatory proteins is essential to distinguish between harmful and beneficial immune response. Cur...
ORGANISM(S): Homo Sapiens 
2018-12-05 | PXD011944 | panorama
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