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ProteomicsA high throughput pipeline for antibody validation
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we show that an inverted version of WB allows parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). The pipeline pro...
ORGANISM(S): Homo sapiens (Human) 
2018-07-26 | PXD010510 | Pride
Lc-ms/ms Antibody validation Ip-ms 
ProteomicsAntibody validation in estrogen receptor beta research
Three antibodies targeting estrogen receptor beta were evaluated with respect to their specificity towards the target protein, by immunoprecipitation followed by LC-Orbitrap MS.
ORGANISM(S): Homo sapiens (Human) 
2017-06-16 | PXD005936 | Pride
Orbitrap ms Ip-ms Estrogen receptor beta Antibody validation 
ProteomicsBaseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation IgG 1:50]
Purpose: There is evidence that therapeutic cancer vaccines can lengthen survival for some cancer patients, but responses vary widely from one person to another. Methods to predict clinical outcomes will advance the field and provide new insights into critical determinants of in vivo efficacy. Thi...
ORGANISM(S): Homo sapiens 
2013-04-01 | E-GEOD-42163 | biostudies-arrayexpress
ProteomicsBaseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation IgM 1:50]
Purpose: There is evidence that therapeutic cancer vaccines can lengthen survival for some cancer patients, but responses vary widely from one person to another. Methods to predict clinical outcomes will advance the field and provide new insights into critical determinants of in vivo efficacy. Thi...
ORGANISM(S): Homo sapiens 
2013-04-01 | E-GEOD-42175 | biostudies-arrayexpress
ProteomicsBaseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation Total Ig 1:200]
Purpose: There is evidence that therapeutic cancer vaccines can lengthen survival for some cancer patients, but responses vary widely from one person to another. Methods to predict clinical outcomes will advance the field and provide new insights into critical determinants of in vivo efficacy. Thi...
ORGANISM(S): Homo sapiens 
2013-04-01 | E-GEOD-42179 | biostudies-arrayexpress
ProteomicsBaseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation Total Ig 1:50]
Purpose: There is evidence that therapeutic cancer vaccines can lengthen survival for some cancer patients, but responses vary widely from one person to another. Methods to predict clinical outcomes will advance the field and provide new insights into critical determinants of in vivo efficacy. Thi...
ORGANISM(S): Homo sapiens 
2013-04-01 | E-GEOD-42176 | biostudies-arrayexpress
ProteomicsProspective ICH Q2(R2)-aligned validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics
This dataset contains raw and processed LC–MS/MS data generated for a prospective validation of label-free untargeted proteomics for host cell protein (HCP) quantification in biotherapeutic matrices. A stable isotope-labeled CHO whole-proteome standard was spiked into the NIST monoclonal antibody re...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD075694 | Pride
Host cell proteins Hcp quantification Biotherapeutics Timstof Analytical validation Untargeted proteomics Ddapasef Ich q2(r2) Lc–ms/ms 
GenomicsPeripheral blood bulk RNA-seq validation cohort for chronic antibody-mediated rejection
Peripheral blood bulk RNA-seq validation cohort for chronic antibody-mediated rejection
PRJNA1476931 | ENA
TranscriptomicsMolecular diagnosis of T cell and/or antibody-mediated rejection in human kidney transplant biopsies
Histologic diagnosis of T cell-mediated rejection in kidney transplant biopsies has limited reproducibility because it is based on non-specific lesions using arbitrary rules that are subject to differing interpretations. We used microarray results from 403 indication biopsies previously given histol...
ORGANISM(S): Homo sapiens 
2013-02-13 | E-GEOD-36059 | biostudies-arrayexpress
GenomicsRestoring the potency of neutralizing antibody via guided hypermutation with hyper-antibody editor
Restoring the potency of neutralizing antibody via guided hypermutation with hyper-antibody editor
PRJNA1182716 | ENA
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