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Site-specifically acetylated G6PD expressed in bacteria or cultured mammalian cells by genetically encoding the incorporation of acetylated lysine. Purified G6PD (expressed in E. coli) or immunopurified G6PD (expressed in HEK293T cells) were subjected to LC-MS/MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD044906 | Pride
HEK293T cells, stably transfected with pyrrolysine UAG suppressor tRNA and aminoacyl tRNA synthetase, for the co-translational incorporation of Boc-protected lysine.
ORGANISM(S): Homo sapiens (Human) 
2020-02-24 | PXD016728 | Pride
To identify acetylation-dependent posttranslational modifications (PTMs) of G6PD, site-specifically acetylated and Flag-tagged G6PD was expressed in HEK293T cells by genetically encoding the incorporation of acetylated lysine in response to an in-frame TAG stop codon. K403-acetylated G6PD (sample) a...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD041775 | Pride
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